Effects of long-term noise exposure during sleep on liver circadian clock and lipid metabolism
BackgroundLong-term exposure to noise during sleep may has adverse effects on metabolic system, and liver lipid metabolism is closely related to circadian clock genes. ObjectiveTo investigate the effects of long-term noise exposure during sleep on liver circadian clock and lipid metabolism in mice a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Editorial Committee of Journal of Environmental and Occupational Medicine
2024-01-01
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| Series: | 环境与职业医学 |
| Subjects: | |
| Online Access: | http://www.jeom.org/article/cn/10.11836/JEOM23189 |
| _version_ | 1797337235796787200 |
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| author | Xinyao ZHANG Xiaojun SHE Yiming FU Bo FU Shuo WANG Mengzhu CHENG Rui WANG Bo CUI |
| author_facet | Xinyao ZHANG Xiaojun SHE Yiming FU Bo FU Shuo WANG Mengzhu CHENG Rui WANG Bo CUI |
| author_sort | Xinyao ZHANG |
| collection | DOAJ |
| description | BackgroundLong-term exposure to noise during sleep may has adverse effects on metabolic system, and liver lipid metabolism is closely related to circadian clock genes. ObjectiveTo investigate the effects of long-term noise exposure during sleep on liver circadian clock and lipid metabolism in mice and its related mechanism. MethodsTwenty C57BL/6J male mice were randomly divided into two groups: a noise exposure group and a control group with 10 mice in each group. The mice in the noise exposure group were exposed to white noise at 90 dB sound pressure level (SPL) for 30 consecutive days, 8 h a day, from 9:00 to 17:00. The mice in the control group were exposed to background noise ≤40 dB SPL. After noise exposure, the animals were neutralized at 14:00 (ZT6) and 2:00 (ZT18), 5 animals at each time spot, and the liver tissues were collected. Total cholesterol and triglyceride in liver were determined by cholesterol oxidase method and glycerol phosphate oxidase method respectively. The expressions of circadian clock genes (Clock, Bmal1, Rev-erbα, and Rev-erbβ) and lipid metabolism genes (Srebp1c, Hmgcr, Fasn, Lxrα, Acc1, and Chrebp) in liver were detected by quantitative real-time PCR. ResultsCompared with the control group, the content of total cholesterol in liver in the noise exposure group increased by 48% (P<0.05) and the content of liver triglyceride increased by 61% (P<0.05) at ZT18. The mRNA expression levels of circadian clock genes Clock and Bmal1 in the noise exposure group was significantly increased at ZT18 and decreased at ZT6 (P<0.05). The mRNA expression level of Rev-erbα decreased at both ZT6 and ZT18 (P<0.05). The mRNA expression level of Rev-erbβ had no significant change at ZT6 and ZT18. The mRNA expression levels of liver lipid metabolism related genes Srebp1c, Hmgcr, Chrebp, and Lxrα in the noise exposure group were higher than those in the control group at ZT18 (P<0.05). The mRNA expression levels of Acc1 and Fasn showed no significant change at ZT6, then an upward trend at ZT18, but no significant difference between the two time spots (P>0.05). ConclusionLong-term noise exposure during sleep can cause circadian clock and lipid metabolism disorders in mice. Among them, suppression of key circadian clock genes may be associated with Rev-erbα-mediated upregulation of the nuclear receptors Srebp1c and Chrebp for lipid synthesis and deposition in the liver, resulting in lipid metabolism disorder. |
| first_indexed | 2024-03-08T09:06:37Z |
| format | Article |
| id | doaj.art-9b21227187674c17aeb463c252d4293e |
| institution | Directory Open Access Journal |
| issn | 2095-9982 |
| language | English |
| last_indexed | 2024-03-08T09:06:37Z |
| publishDate | 2024-01-01 |
| publisher | Editorial Committee of Journal of Environmental and Occupational Medicine |
| record_format | Article |
| series | 环境与职业医学 |
| spelling | doaj.art-9b21227187674c17aeb463c252d4293e2024-02-01T05:11:47ZengEditorial Committee of Journal of Environmental and Occupational Medicine环境与职业医学2095-99822024-01-01411414610.11836/JEOM2318923189Effects of long-term noise exposure during sleep on liver circadian clock and lipid metabolismXinyao ZHANG0Xiaojun SHE1Yiming FU2Bo FU3Shuo WANG4Mengzhu CHENG5Rui WANG6Bo CUI7Shandong Academy of Occupational Health and Occupational Medicine, Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan, Shandong 250062, ChinaInstitute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin 300050, ChinaInstitute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin 300050, ChinaInstitute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin 300050, ChinaShandong Academy of Occupational Health and Occupational Medicine, Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan, Shandong 250062, ChinaInstitute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin 300050, ChinaShandong Academy of Occupational Health and Occupational Medicine, Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan, Shandong 250062, ChinaInstitute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin 300050, ChinaBackgroundLong-term exposure to noise during sleep may has adverse effects on metabolic system, and liver lipid metabolism is closely related to circadian clock genes. ObjectiveTo investigate the effects of long-term noise exposure during sleep on liver circadian clock and lipid metabolism in mice and its related mechanism. MethodsTwenty C57BL/6J male mice were randomly divided into two groups: a noise exposure group and a control group with 10 mice in each group. The mice in the noise exposure group were exposed to white noise at 90 dB sound pressure level (SPL) for 30 consecutive days, 8 h a day, from 9:00 to 17:00. The mice in the control group were exposed to background noise ≤40 dB SPL. After noise exposure, the animals were neutralized at 14:00 (ZT6) and 2:00 (ZT18), 5 animals at each time spot, and the liver tissues were collected. Total cholesterol and triglyceride in liver were determined by cholesterol oxidase method and glycerol phosphate oxidase method respectively. The expressions of circadian clock genes (Clock, Bmal1, Rev-erbα, and Rev-erbβ) and lipid metabolism genes (Srebp1c, Hmgcr, Fasn, Lxrα, Acc1, and Chrebp) in liver were detected by quantitative real-time PCR. ResultsCompared with the control group, the content of total cholesterol in liver in the noise exposure group increased by 48% (P<0.05) and the content of liver triglyceride increased by 61% (P<0.05) at ZT18. The mRNA expression levels of circadian clock genes Clock and Bmal1 in the noise exposure group was significantly increased at ZT18 and decreased at ZT6 (P<0.05). The mRNA expression level of Rev-erbα decreased at both ZT6 and ZT18 (P<0.05). The mRNA expression level of Rev-erbβ had no significant change at ZT6 and ZT18. The mRNA expression levels of liver lipid metabolism related genes Srebp1c, Hmgcr, Chrebp, and Lxrα in the noise exposure group were higher than those in the control group at ZT18 (P<0.05). The mRNA expression levels of Acc1 and Fasn showed no significant change at ZT6, then an upward trend at ZT18, but no significant difference between the two time spots (P>0.05). ConclusionLong-term noise exposure during sleep can cause circadian clock and lipid metabolism disorders in mice. Among them, suppression of key circadian clock genes may be associated with Rev-erbα-mediated upregulation of the nuclear receptors Srebp1c and Chrebp for lipid synthesis and deposition in the liver, resulting in lipid metabolism disorder.http://www.jeom.org/article/cn/10.11836/JEOM23189sleep noisecircadian clocklipid metabolismliver |
| spellingShingle | Xinyao ZHANG Xiaojun SHE Yiming FU Bo FU Shuo WANG Mengzhu CHENG Rui WANG Bo CUI Effects of long-term noise exposure during sleep on liver circadian clock and lipid metabolism 环境与职业医学 sleep noise circadian clock lipid metabolism liver |
| title | Effects of long-term noise exposure during sleep on liver circadian clock and lipid metabolism |
| title_full | Effects of long-term noise exposure during sleep on liver circadian clock and lipid metabolism |
| title_fullStr | Effects of long-term noise exposure during sleep on liver circadian clock and lipid metabolism |
| title_full_unstemmed | Effects of long-term noise exposure during sleep on liver circadian clock and lipid metabolism |
| title_short | Effects of long-term noise exposure during sleep on liver circadian clock and lipid metabolism |
| title_sort | effects of long term noise exposure during sleep on liver circadian clock and lipid metabolism |
| topic | sleep noise circadian clock lipid metabolism liver |
| url | http://www.jeom.org/article/cn/10.11836/JEOM23189 |
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