Testing the Induction of Metritis in Healthy Postpartum Primiparous Cows Challenged with a Cocktail of Bacteria
Metritis is a postpartum uterine disease with greater incidence in primiparous than in multiparous cows. In primiparous cows, the impact on production and health is lessened, presumably due to a superior immune response. Here, we tested whether an in vivo model of clinical metritis induction develop...
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MDPI AG
2023-09-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/18/2852 |
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| author | Josiane C. C. Silva Leonardo Bringhenti Lucas C. Siqueira Marjory X. Rodrigues Martin Zinicola Brianna Pomeroy Rodrigo C. Bicalho |
| author_facet | Josiane C. C. Silva Leonardo Bringhenti Lucas C. Siqueira Marjory X. Rodrigues Martin Zinicola Brianna Pomeroy Rodrigo C. Bicalho |
| author_sort | Josiane C. C. Silva |
| collection | DOAJ |
| description | Metritis is a postpartum uterine disease with greater incidence in primiparous than in multiparous cows. In primiparous cows, the impact on production and health is lessened, presumably due to a superior immune response. Here, we tested whether an in vivo model of clinical metritis induction developed for postpartum multiparous Holstein cows would produce similar results in primiparous cows. Thirty-six cows were randomly assigned to one of three groups and received intrauterine infusion within 24 h of parturition. The controls were infused with sterile saline; the low-dose group received a bacterial cocktail containing 10<sup>3</sup> cfu of <i>Escherichia coli</i>, <i>Trueperella pyogenes</i>, and <i>Fusobacterium necrophorum</i>; and the high-dose group were infused with 10<sup>6</sup> cfu of the same cocktail. Production, health traits, and the vaginal discharge culture were assessed daily, from enrollment until 14 d in milk. Clinical metritis occurred in 64% of high-dose cows, 33% of the controls, and 42% of low-dose cows, with no significant difference of incidence between groups. However, when accounting by time, high-dose cows had a 2.7 times greater hazard of metritis compared with the controls. The bacterial challenge affected milk production and dry matter intake tended to decrease. In the high-dose group, a greater growth <i>of F. necrophorum</i> in the selective medium was also observed, suggesting an association with metritis. Therefore, this study suggests intrauterine inoculation with 10<sup>6</sup> cfu of this bacterial cocktail elicits physical and clinical outcomes consistent with clinical metritis. |
| first_indexed | 2024-03-10T23:07:41Z |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T23:07:41Z |
| publishDate | 2023-09-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-9b2299898ae340329b07f7612522b6ba2023-11-19T09:14:31ZengMDPI AGAnimals2076-26152023-09-011318285210.3390/ani13182852Testing the Induction of Metritis in Healthy Postpartum Primiparous Cows Challenged with a Cocktail of BacteriaJosiane C. C. Silva0Leonardo Bringhenti1Lucas C. Siqueira2Marjory X. Rodrigues3Martin Zinicola4Brianna Pomeroy5Rodrigo C. Bicalho6Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14853, USADepartment of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14853, USADepartment of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14853, USADepartment of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14853, USAZoetis Animal Health, Kalamazoo, MI 49007, USAZoetis Animal Health, Kalamazoo, MI 49007, USADepartment of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14853, USAMetritis is a postpartum uterine disease with greater incidence in primiparous than in multiparous cows. In primiparous cows, the impact on production and health is lessened, presumably due to a superior immune response. Here, we tested whether an in vivo model of clinical metritis induction developed for postpartum multiparous Holstein cows would produce similar results in primiparous cows. Thirty-six cows were randomly assigned to one of three groups and received intrauterine infusion within 24 h of parturition. The controls were infused with sterile saline; the low-dose group received a bacterial cocktail containing 10<sup>3</sup> cfu of <i>Escherichia coli</i>, <i>Trueperella pyogenes</i>, and <i>Fusobacterium necrophorum</i>; and the high-dose group were infused with 10<sup>6</sup> cfu of the same cocktail. Production, health traits, and the vaginal discharge culture were assessed daily, from enrollment until 14 d in milk. Clinical metritis occurred in 64% of high-dose cows, 33% of the controls, and 42% of low-dose cows, with no significant difference of incidence between groups. However, when accounting by time, high-dose cows had a 2.7 times greater hazard of metritis compared with the controls. The bacterial challenge affected milk production and dry matter intake tended to decrease. In the high-dose group, a greater growth <i>of F. necrophorum</i> in the selective medium was also observed, suggesting an association with metritis. Therefore, this study suggests intrauterine inoculation with 10<sup>6</sup> cfu of this bacterial cocktail elicits physical and clinical outcomes consistent with clinical metritis.https://www.mdpi.com/2076-2615/13/18/2852metritischallengemicrobiotaprimiparousdairy cow |
| spellingShingle | Josiane C. C. Silva Leonardo Bringhenti Lucas C. Siqueira Marjory X. Rodrigues Martin Zinicola Brianna Pomeroy Rodrigo C. Bicalho Testing the Induction of Metritis in Healthy Postpartum Primiparous Cows Challenged with a Cocktail of Bacteria Animals metritis challenge microbiota primiparous dairy cow |
| title | Testing the Induction of Metritis in Healthy Postpartum Primiparous Cows Challenged with a Cocktail of Bacteria |
| title_full | Testing the Induction of Metritis in Healthy Postpartum Primiparous Cows Challenged with a Cocktail of Bacteria |
| title_fullStr | Testing the Induction of Metritis in Healthy Postpartum Primiparous Cows Challenged with a Cocktail of Bacteria |
| title_full_unstemmed | Testing the Induction of Metritis in Healthy Postpartum Primiparous Cows Challenged with a Cocktail of Bacteria |
| title_short | Testing the Induction of Metritis in Healthy Postpartum Primiparous Cows Challenged with a Cocktail of Bacteria |
| title_sort | testing the induction of metritis in healthy postpartum primiparous cows challenged with a cocktail of bacteria |
| topic | metritis challenge microbiota primiparous dairy cow |
| url | https://www.mdpi.com/2076-2615/13/18/2852 |
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