Lantibiotics Produced by Oral Inhabitants as a Trigger for Dysbiosis of Human Intestinal Microbiota
Lantibiotics are a type of bacteriocin produced by Gram-positive bacteria and have a wide spectrum of Gram-positive antimicrobial activity. In this study, we determined that Mutacin I/III and Smb (a dipeptide lantibiotic), which are mainly produced by the widespread cariogenic bacterium <i>Str...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/7/3343 |
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| author | Hideo Yonezawa Mizuho Motegi Atsushi Oishi Fuhito Hojo Seiya Higashi Eriko Nozaki Kentaro Oka Motomichi Takahashi Takako Osaki Shigeru Kamiya |
| author_facet | Hideo Yonezawa Mizuho Motegi Atsushi Oishi Fuhito Hojo Seiya Higashi Eriko Nozaki Kentaro Oka Motomichi Takahashi Takako Osaki Shigeru Kamiya |
| author_sort | Hideo Yonezawa |
| collection | DOAJ |
| description | Lantibiotics are a type of bacteriocin produced by Gram-positive bacteria and have a wide spectrum of Gram-positive antimicrobial activity. In this study, we determined that Mutacin I/III and Smb (a dipeptide lantibiotic), which are mainly produced by the widespread cariogenic bacterium <i>Streptococcus mutans</i>, have strong antimicrobial activities against many of the Gram-positive bacteria which constitute the intestinal microbiota. These lantibiotics also demonstrate resistance to acid and temperature. Based on these features, we predicted that lantibiotics may be able to persist into the intestinal tract maintaining a strong antimicrobial activity, affecting the intestinal microbiota. Saliva and fecal samples from 69 subjects were collected to test this hypothesis and the presence of lantibiotics and the composition of the intestinal microbiota were examined. We demonstrate that subjects possessing lantibiotic-producing bacteria in their oral cavity exhibited a tendency of decreased species richness and have significantly reduced abundance of the phylum Firmicutes in their intestinal microbiota. Similar results were obtained in the fecal microbiota of mice fed with <i>S. mutans</i> culture supernatant containing the lantibiotic bacteriocin Mutacin I. These results showed that lantibiotic bacteriocins produced in the oral cavity perturb the intestinal microbiota and suggest that oral bacteria may be one of the causative factors of intestinal microbiota dysbiosis. |
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| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:56:20Z |
| publishDate | 2021-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9b23fa1886074329953a43e6991ca46b2023-11-21T11:55:15ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01227334310.3390/ijms22073343Lantibiotics Produced by Oral Inhabitants as a Trigger for Dysbiosis of Human Intestinal MicrobiotaHideo Yonezawa0Mizuho Motegi1Atsushi Oishi2Fuhito Hojo3Seiya Higashi4Eriko Nozaki5Kentaro Oka6Motomichi Takahashi7Takako Osaki8Shigeru Kamiya9Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo 181-8611, JapanDivision of Oral Restitution, Department of Pediatric Dentistry, Graduate School, Tokyo Medical and Dental University, Tokyo 113-8510, JapanDivision of Oral Restitution, Department of Pediatric Dentistry, Graduate School, Tokyo Medical and Dental University, Tokyo 113-8510, JapanInstitute of Laboratory Animals, Graduate School of Medicine, Kyorin University School of Medicine, Tokyo 181-8611, JapanCentral Research Institute, Miyarisan Pharmaceutical Co. Ltd., Tokyo 114-0016, JapanCore Laboratory for Proteomics and Genomics, Kyorin University School of Medicine, Tokyo 181-8611, JapanCentral Research Institute, Miyarisan Pharmaceutical Co. Ltd., Tokyo 114-0016, JapanDepartment of Infectious Diseases, Kyorin University School of Medicine, Tokyo 181-8611, JapanDepartment of Infectious Diseases, Kyorin University School of Medicine, Tokyo 181-8611, JapanDepartment of Infectious Diseases, Kyorin University School of Medicine, Tokyo 181-8611, JapanLantibiotics are a type of bacteriocin produced by Gram-positive bacteria and have a wide spectrum of Gram-positive antimicrobial activity. In this study, we determined that Mutacin I/III and Smb (a dipeptide lantibiotic), which are mainly produced by the widespread cariogenic bacterium <i>Streptococcus mutans</i>, have strong antimicrobial activities against many of the Gram-positive bacteria which constitute the intestinal microbiota. These lantibiotics also demonstrate resistance to acid and temperature. Based on these features, we predicted that lantibiotics may be able to persist into the intestinal tract maintaining a strong antimicrobial activity, affecting the intestinal microbiota. Saliva and fecal samples from 69 subjects were collected to test this hypothesis and the presence of lantibiotics and the composition of the intestinal microbiota were examined. We demonstrate that subjects possessing lantibiotic-producing bacteria in their oral cavity exhibited a tendency of decreased species richness and have significantly reduced abundance of the phylum Firmicutes in their intestinal microbiota. Similar results were obtained in the fecal microbiota of mice fed with <i>S. mutans</i> culture supernatant containing the lantibiotic bacteriocin Mutacin I. These results showed that lantibiotic bacteriocins produced in the oral cavity perturb the intestinal microbiota and suggest that oral bacteria may be one of the causative factors of intestinal microbiota dysbiosis.https://www.mdpi.com/1422-0067/22/7/3343lantibioticsMutacinSmboral bacteriaintestinal microbiotadysbiosis |
| spellingShingle | Hideo Yonezawa Mizuho Motegi Atsushi Oishi Fuhito Hojo Seiya Higashi Eriko Nozaki Kentaro Oka Motomichi Takahashi Takako Osaki Shigeru Kamiya Lantibiotics Produced by Oral Inhabitants as a Trigger for Dysbiosis of Human Intestinal Microbiota International Journal of Molecular Sciences lantibiotics Mutacin Smb oral bacteria intestinal microbiota dysbiosis |
| title | Lantibiotics Produced by Oral Inhabitants as a Trigger for Dysbiosis of Human Intestinal Microbiota |
| title_full | Lantibiotics Produced by Oral Inhabitants as a Trigger for Dysbiosis of Human Intestinal Microbiota |
| title_fullStr | Lantibiotics Produced by Oral Inhabitants as a Trigger for Dysbiosis of Human Intestinal Microbiota |
| title_full_unstemmed | Lantibiotics Produced by Oral Inhabitants as a Trigger for Dysbiosis of Human Intestinal Microbiota |
| title_short | Lantibiotics Produced by Oral Inhabitants as a Trigger for Dysbiosis of Human Intestinal Microbiota |
| title_sort | lantibiotics produced by oral inhabitants as a trigger for dysbiosis of human intestinal microbiota |
| topic | lantibiotics Mutacin Smb oral bacteria intestinal microbiota dysbiosis |
| url | https://www.mdpi.com/1422-0067/22/7/3343 |
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