Infrared spectroscopy of serum fails to identify early biomarker changes in an equine model of traumatic osteoarthritis
Objective: to determine the accuracy of infrared (IR)-based serum biomarker profiling to differentiate horses with early inflammatory changes associated with a traumatically induced model of equine carpal osteoarthritis (OA) from controls.Method: unilateral carpal OA was induced in 9 of 17 healthy T...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Osteoarthritis and Cartilage Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665913122000656 |
| _version_ | 1797991900378038272 |
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| author | L. Panizzi M. Vignes K.E. Dittmer M.R. Waterland C.W. Rogers H. Sano C.W. McIlwraith S. Pemberton M. Owen C.B. Riley |
| author_facet | L. Panizzi M. Vignes K.E. Dittmer M.R. Waterland C.W. Rogers H. Sano C.W. McIlwraith S. Pemberton M. Owen C.B. Riley |
| author_sort | L. Panizzi |
| collection | DOAJ |
| description | Objective: to determine the accuracy of infrared (IR)-based serum biomarker profiling to differentiate horses with early inflammatory changes associated with a traumatically induced model of equine carpal osteoarthritis (OA) from controls.Method: unilateral carpal OA was induced in 9 of 17 healthy Thoroughbred fillies, while the remainder served as sham operated controls. Serum samples were obtained before induction of OA (Day 0) and weekly thereafter until Day 63 from both groups. Films of dried serum were created, and IR absorbance spectra acquired. Following pre-processing, partial least squares discriminant analysis (PLSDA) and principal component analysis (PCA) were used to assess group and time differences and generate predictive models for wavenumber ranges 1300-1800 cm−1 and 2600-3700 cm−1.Results: the overall correct classification rate when classifying samples by group (OA or Sham) was 52.7% (s.d. = 12.8%), while it was 94.0% (s.d. = 1.4%) by sampling Day. The correct classification results by group-sampling Day combinations with pre-intervention serum (Day 0) was 50.5% (s.d. = 21.7%).Conclusion: with the current approach IR spectroscopic analysis could not differentiate serum of horses with induced carpal OA from that of controls. The high classification rate obtained by Day of sampling may reflect the effect of exercise on the biomarker profile. A longer study period (advanced disease) or naturally occurring disease may provide further information on the suitability of this technique in horses. |
| first_indexed | 2024-04-11T08:58:34Z |
| format | Article |
| id | doaj.art-9b2b32e9915840908b2a79502336521a |
| institution | Directory Open Access Journal |
| issn | 2665-9131 |
| language | English |
| last_indexed | 2024-04-11T08:58:34Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Osteoarthritis and Cartilage Open |
| spelling | doaj.art-9b2b32e9915840908b2a79502336521a2022-12-22T04:32:56ZengElsevierOsteoarthritis and Cartilage Open2665-91312022-12-0144100297Infrared spectroscopy of serum fails to identify early biomarker changes in an equine model of traumatic osteoarthritisL. Panizzi0M. Vignes1K.E. Dittmer2M.R. Waterland3C.W. Rogers4H. Sano5C.W. McIlwraith6S. Pemberton7M. Owen8C.B. Riley9School of Veterinary Science, Massey University, Palmerston North, New Zealand; Corresponding author.School of Mathematical and Computational Sciences, Massey University, Palmerston North, New ZealandSchool of Veterinary Science, Massey University, Palmerston North, New ZealandSchool of Natural Sciences, Massey University, Palmerston North, New ZealandSchool of Veterinary Science, Massey University, Palmerston North, New Zealand; School of Agriculture and Environment, Massey University, Palmerston North, New ZealandSchool of Veterinary Science, Massey University, Palmerston North, New ZealandOrthopaedic Research Center, C. Wayne McIlwraith Translational Medicine Institute, Colorado State University, School of Veterinary Medicine, Colorado, USAVet 20/20, Palmerston North, New ZealandNZRadVet Ltd, Feilding, New ZealandSchool of Veterinary Science, Massey University, Palmerston North, New ZealandObjective: to determine the accuracy of infrared (IR)-based serum biomarker profiling to differentiate horses with early inflammatory changes associated with a traumatically induced model of equine carpal osteoarthritis (OA) from controls.Method: unilateral carpal OA was induced in 9 of 17 healthy Thoroughbred fillies, while the remainder served as sham operated controls. Serum samples were obtained before induction of OA (Day 0) and weekly thereafter until Day 63 from both groups. Films of dried serum were created, and IR absorbance spectra acquired. Following pre-processing, partial least squares discriminant analysis (PLSDA) and principal component analysis (PCA) were used to assess group and time differences and generate predictive models for wavenumber ranges 1300-1800 cm−1 and 2600-3700 cm−1.Results: the overall correct classification rate when classifying samples by group (OA or Sham) was 52.7% (s.d. = 12.8%), while it was 94.0% (s.d. = 1.4%) by sampling Day. The correct classification results by group-sampling Day combinations with pre-intervention serum (Day 0) was 50.5% (s.d. = 21.7%).Conclusion: with the current approach IR spectroscopic analysis could not differentiate serum of horses with induced carpal OA from that of controls. The high classification rate obtained by Day of sampling may reflect the effect of exercise on the biomarker profile. A longer study period (advanced disease) or naturally occurring disease may provide further information on the suitability of this technique in horses.http://www.sciencedirect.com/science/article/pii/S2665913122000656SpectroscopyEquineSerumBiomarkerOsteoarthritis |
| spellingShingle | L. Panizzi M. Vignes K.E. Dittmer M.R. Waterland C.W. Rogers H. Sano C.W. McIlwraith S. Pemberton M. Owen C.B. Riley Infrared spectroscopy of serum fails to identify early biomarker changes in an equine model of traumatic osteoarthritis Osteoarthritis and Cartilage Open Spectroscopy Equine Serum Biomarker Osteoarthritis |
| title | Infrared spectroscopy of serum fails to identify early biomarker changes in an equine model of traumatic osteoarthritis |
| title_full | Infrared spectroscopy of serum fails to identify early biomarker changes in an equine model of traumatic osteoarthritis |
| title_fullStr | Infrared spectroscopy of serum fails to identify early biomarker changes in an equine model of traumatic osteoarthritis |
| title_full_unstemmed | Infrared spectroscopy of serum fails to identify early biomarker changes in an equine model of traumatic osteoarthritis |
| title_short | Infrared spectroscopy of serum fails to identify early biomarker changes in an equine model of traumatic osteoarthritis |
| title_sort | infrared spectroscopy of serum fails to identify early biomarker changes in an equine model of traumatic osteoarthritis |
| topic | Spectroscopy Equine Serum Biomarker Osteoarthritis |
| url | http://www.sciencedirect.com/science/article/pii/S2665913122000656 |
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