Mechanical assessment and odontogenic behavior of a 3D-printed mesoporous calcium silicate/calcium sulfate/poly-ε-caprolactone composite scaffold
Background/purpose: Tissue engineering in dentistry has fundamentally changed the way endodontists assess treatment options. Our previous study found that quercetin-contained mesoporous calcium silicate/calcium sulfate (MSCSQ) could induce hard tissue defect region regeneration. This study focused o...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-02-01
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| Series: | Journal of the Formosan Medical Association |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664621003053 |
| _version_ | 1818874256881090560 |
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| author | Chun-Liang Yeh Rupali Bhorade Tuan-Ti Hsu Cheng-Yu Chen Chun-Pin Lin |
| author_facet | Chun-Liang Yeh Rupali Bhorade Tuan-Ti Hsu Cheng-Yu Chen Chun-Pin Lin |
| author_sort | Chun-Liang Yeh |
| collection | DOAJ |
| description | Background/purpose: Tissue engineering in dentistry has fundamentally changed the way endodontists assess treatment options. Our previous study found that quercetin-contained mesoporous calcium silicate/calcium sulfate (MSCSQ) could induce hard tissue defect region regeneration. This study focused on whether the MSCSQ scaffold could also be effective in regulating odontogenesis and dentin regeneration. Methods: In this study, we fabricated MSCSQ composite scaffolds using the 3D printing technique. The characteristics of the MSCSQ scaffold were examined by scanning electron microscope (SEM), and mechanical properties were also assessed. In addition, we evaluated the cell viability, cell proliferation, odontogenic-related protein expression, and mineralization behavior of human dental pulp stem cells (hDPSCs) cultured on different scaffolds. Results: We found the precipitation of spherical-apatite on the scaffold surface rapidly in short periods. The in-vitro results for cell behavior revealed that hDPSCs with an MSCSQ scaffold were significantly higher in cell viability as followed time points. In addition, the specific makers of odontogenesis, such as DSPP and DMP-1 proteins, were induced obviously after culturing the hDPSCs on the MSCSQ scaffold. Conclusion: Our results demonstrated that MSCSQ scaffolds could enhance physicochemical and biological behaviors compared to mesoporous calcium silicate/calcium sulfate (MSCS) scaffolds. In addition, MSCSQ scaffolds also enhanced odontogenic and immuno-suppressive properties compared to MSCS scaffolds. These results indicated that MSCSQ scaffolds could be considered a potential bioscaffold for clinical applications and dentin regeneration. |
| first_indexed | 2024-12-19T13:07:43Z |
| format | Article |
| id | doaj.art-9b32ca052c7d423e99134e8e0e78ca50 |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-19T13:07:43Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-9b32ca052c7d423e99134e8e0e78ca502022-12-21T20:20:00ZengElsevierJournal of the Formosan Medical Association0929-66462022-02-011212510518Mechanical assessment and odontogenic behavior of a 3D-printed mesoporous calcium silicate/calcium sulfate/poly-ε-caprolactone composite scaffoldChun-Liang Yeh0Rupali Bhorade1Tuan-Ti Hsu2Cheng-Yu Chen3Chun-Pin Lin4Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, TaiwanGraduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, TaiwanX-Dimension Center for Medical Research and Translation, China Medical University Hospital, Taichung, TaiwanGraduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, TaiwanGraduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; Corresponding author. Department of Dentistry, National Taiwan University Hospital, No. 1 Chang-De Street, Taipei, 10048, Taiwan. Fax: +886 2 23831346.Background/purpose: Tissue engineering in dentistry has fundamentally changed the way endodontists assess treatment options. Our previous study found that quercetin-contained mesoporous calcium silicate/calcium sulfate (MSCSQ) could induce hard tissue defect region regeneration. This study focused on whether the MSCSQ scaffold could also be effective in regulating odontogenesis and dentin regeneration. Methods: In this study, we fabricated MSCSQ composite scaffolds using the 3D printing technique. The characteristics of the MSCSQ scaffold were examined by scanning electron microscope (SEM), and mechanical properties were also assessed. In addition, we evaluated the cell viability, cell proliferation, odontogenic-related protein expression, and mineralization behavior of human dental pulp stem cells (hDPSCs) cultured on different scaffolds. Results: We found the precipitation of spherical-apatite on the scaffold surface rapidly in short periods. The in-vitro results for cell behavior revealed that hDPSCs with an MSCSQ scaffold were significantly higher in cell viability as followed time points. In addition, the specific makers of odontogenesis, such as DSPP and DMP-1 proteins, were induced obviously after culturing the hDPSCs on the MSCSQ scaffold. Conclusion: Our results demonstrated that MSCSQ scaffolds could enhance physicochemical and biological behaviors compared to mesoporous calcium silicate/calcium sulfate (MSCS) scaffolds. In addition, MSCSQ scaffolds also enhanced odontogenic and immuno-suppressive properties compared to MSCS scaffolds. These results indicated that MSCSQ scaffolds could be considered a potential bioscaffold for clinical applications and dentin regeneration.http://www.sciencedirect.com/science/article/pii/S0929664621003053Quercetin3D printingDentin regenerationOdontogenesisMesoporous calcium silicate |
| spellingShingle | Chun-Liang Yeh Rupali Bhorade Tuan-Ti Hsu Cheng-Yu Chen Chun-Pin Lin Mechanical assessment and odontogenic behavior of a 3D-printed mesoporous calcium silicate/calcium sulfate/poly-ε-caprolactone composite scaffold Journal of the Formosan Medical Association Quercetin 3D printing Dentin regeneration Odontogenesis Mesoporous calcium silicate |
| title | Mechanical assessment and odontogenic behavior of a 3D-printed mesoporous calcium silicate/calcium sulfate/poly-ε-caprolactone composite scaffold |
| title_full | Mechanical assessment and odontogenic behavior of a 3D-printed mesoporous calcium silicate/calcium sulfate/poly-ε-caprolactone composite scaffold |
| title_fullStr | Mechanical assessment and odontogenic behavior of a 3D-printed mesoporous calcium silicate/calcium sulfate/poly-ε-caprolactone composite scaffold |
| title_full_unstemmed | Mechanical assessment and odontogenic behavior of a 3D-printed mesoporous calcium silicate/calcium sulfate/poly-ε-caprolactone composite scaffold |
| title_short | Mechanical assessment and odontogenic behavior of a 3D-printed mesoporous calcium silicate/calcium sulfate/poly-ε-caprolactone composite scaffold |
| title_sort | mechanical assessment and odontogenic behavior of a 3d printed mesoporous calcium silicate calcium sulfate poly ε caprolactone composite scaffold |
| topic | Quercetin 3D printing Dentin regeneration Odontogenesis Mesoporous calcium silicate |
| url | http://www.sciencedirect.com/science/article/pii/S0929664621003053 |
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