Effects of cyclophosphamide on proliferation and viability of malignant HN5 cell line and comparing with embryonic fibroblast cells
Background: One of the major causes of death in the world is cancer and therefore any study in the field of cancer biology is of great importance. Head and neck cancers represent approximately 2-5% of neoplasms which is higher in some countries. The most appropriate therapy for various cancers is id...
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| Format: | Article |
| Language: | fas |
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Tehran University of Medical Sciences
2017-02-01
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| Series: | Tehran University Medical Journal |
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| Online Access: | http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5531&slc_lang=en&sid=1 |
| _version_ | 1819180131323740160 |
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| author | Elham Hoveizi Tayebeh Mohammadi |
| author_facet | Elham Hoveizi Tayebeh Mohammadi |
| author_sort | Elham Hoveizi |
| collection | DOAJ |
| description | Background: One of the major causes of death in the world is cancer and therefore any study in the field of cancer biology is of great importance. Head and neck cancers represent approximately 2-5% of neoplasms which is higher in some countries. The most appropriate therapy for various cancers is identifying effective and efficient ways that contribute to initiation of apoptosis. Cyclophosphamide is an alkylating agent that stops the replication of DNA and then, it stops the cell proliferation and viability. Therefore, cyclophosphamide is used to treat various types of cancer. In this study we evaluate the cytotoxic effects of cyclophosphamide on viability of (head and neck cancer cells) HN5 cell line and compare it with fibroblast cells as noncancerous cells.
Methods: This experimental study was done in cell and developmental laboratory in faculty of science, Shahid Chamran University of Ahvaz in Spring of 2016. HN5 cell line and embryonic fibroblast cells were grown in DMEM supplemented with 10% fetal bovine serum (FBS), penicillin/streptomycin (100 U/ml, 100 µg/ml) at 37 °C, then the effects of different concentrations of cyclophosphamide on cell viability was evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. 4',6-diamidino-2-phenylindole (DAPI) staining was performed to determine the proportion of apoptotic cells by manually counting pyknotic nuclei. According to standard procedures from day 13 embryos of outbred strains naval medical research institute (NMRI), fibroblast cells were isolated. In this study HN5 cell line and fibroblasts were exposed to cytostatics for 72 hours.
Results: Various concentrations of cyclophosphamide were effective in cytotoxicity of HN5 cancer and fibroblast cells. A significant cytotoxicity was observed with the examined concentration of 1 µg/ml of cyclophosphamide with 50% in 3th day and P< 0.001. Interestingly, at low concentrations, cyclophosphamide was more toxic than at higher concentrations.
Conclusion: Totally cyclophosphamide had low toxicity effects on both of the cell lines but the toxicity effects of cyclophosphamide on HN5 were significantly greater than fibroblast cells. These results indicate that cyclophosphamide can be a potential anticancer agent. |
| first_indexed | 2024-12-22T22:09:28Z |
| format | Article |
| id | doaj.art-9b34e96a2c264f52a4f93262bf337963 |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-12-22T22:09:28Z |
| publishDate | 2017-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-9b34e96a2c264f52a4f93262bf3379632022-12-21T18:10:54ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222017-02-017411763770Effects of cyclophosphamide on proliferation and viability of malignant HN5 cell line and comparing with embryonic fibroblast cellsElham Hoveizi0Tayebeh Mohammadi1 Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran. Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran. Background: One of the major causes of death in the world is cancer and therefore any study in the field of cancer biology is of great importance. Head and neck cancers represent approximately 2-5% of neoplasms which is higher in some countries. The most appropriate therapy for various cancers is identifying effective and efficient ways that contribute to initiation of apoptosis. Cyclophosphamide is an alkylating agent that stops the replication of DNA and then, it stops the cell proliferation and viability. Therefore, cyclophosphamide is used to treat various types of cancer. In this study we evaluate the cytotoxic effects of cyclophosphamide on viability of (head and neck cancer cells) HN5 cell line and compare it with fibroblast cells as noncancerous cells. Methods: This experimental study was done in cell and developmental laboratory in faculty of science, Shahid Chamran University of Ahvaz in Spring of 2016. HN5 cell line and embryonic fibroblast cells were grown in DMEM supplemented with 10% fetal bovine serum (FBS), penicillin/streptomycin (100 U/ml, 100 µg/ml) at 37 °C, then the effects of different concentrations of cyclophosphamide on cell viability was evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. 4',6-diamidino-2-phenylindole (DAPI) staining was performed to determine the proportion of apoptotic cells by manually counting pyknotic nuclei. According to standard procedures from day 13 embryos of outbred strains naval medical research institute (NMRI), fibroblast cells were isolated. In this study HN5 cell line and fibroblasts were exposed to cytostatics for 72 hours. Results: Various concentrations of cyclophosphamide were effective in cytotoxicity of HN5 cancer and fibroblast cells. A significant cytotoxicity was observed with the examined concentration of 1 µg/ml of cyclophosphamide with 50% in 3th day and P< 0.001. Interestingly, at low concentrations, cyclophosphamide was more toxic than at higher concentrations. Conclusion: Totally cyclophosphamide had low toxicity effects on both of the cell lines but the toxicity effects of cyclophosphamide on HN5 were significantly greater than fibroblast cells. These results indicate that cyclophosphamide can be a potential anticancer agent.http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5531&slc_lang=en&sid=1cancer cell viability cyclophosphamide |
| spellingShingle | Elham Hoveizi Tayebeh Mohammadi Effects of cyclophosphamide on proliferation and viability of malignant HN5 cell line and comparing with embryonic fibroblast cells Tehran University Medical Journal cancer cell viability cyclophosphamide |
| title | Effects of cyclophosphamide on proliferation and viability of malignant HN5 cell line and comparing with embryonic fibroblast cells |
| title_full | Effects of cyclophosphamide on proliferation and viability of malignant HN5 cell line and comparing with embryonic fibroblast cells |
| title_fullStr | Effects of cyclophosphamide on proliferation and viability of malignant HN5 cell line and comparing with embryonic fibroblast cells |
| title_full_unstemmed | Effects of cyclophosphamide on proliferation and viability of malignant HN5 cell line and comparing with embryonic fibroblast cells |
| title_short | Effects of cyclophosphamide on proliferation and viability of malignant HN5 cell line and comparing with embryonic fibroblast cells |
| title_sort | effects of cyclophosphamide on proliferation and viability of malignant hn5 cell line and comparing with embryonic fibroblast cells |
| topic | cancer cell viability cyclophosphamide |
| url | http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5531&slc_lang=en&sid=1 |
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