Dissemination Of t437-SCCmecIV And Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus aureus Isolated From Skin Infections And Diabetic Foot Ulcers
Maria Stańkowska,1 Katarzyna Garbacz,1 Lidia Piechowicz,2 Marek Bronk3 1Department of Oral Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland; 2Department of Medical Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland; 3Laboratory of Clinical...
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Dove Medical Press
2019-10-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/dissemination-of-t437-sccmeciv-and-coagulase-negative-t037-sccmeciii-t-peer-reviewed-article-IDR |
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| author | Stańkowska M Garbacz K Piechowicz L Bronk M |
| author_facet | Stańkowska M Garbacz K Piechowicz L Bronk M |
| author_sort | Stańkowska M |
| collection | DOAJ |
| description | Maria Stańkowska,1 Katarzyna Garbacz,1 Lidia Piechowicz,2 Marek Bronk3 1Department of Oral Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland; 2Department of Medical Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland; 3Laboratory of Clinical Microbiology, University Clinical Center, Gdansk, PolandCorrespondence: Katarzyna GarbaczDepartment of Oral Microbiology, Medical University of Gdansk, 25 Dębowa St, Gdansk 80-204, PolandTel +48 58 349 1900Fax +48 58 349 1668Email katarzyna.garbacz@gumed.edu.plBackground: In a recent decade, the occurrence of S. aureus isolates with low-level oxacillin resistance, have been reported increasingly. The aim of this study was to estimate the prevalence of S. aureus with low-level of oxacillin resistance and to determine their molecular characteristics, including spa types, SCCmec types and presence of toxin genes.Methods: A total of 249 S. aureus strains were analyzed. Antimicrobial susceptibility was preliminarily tested by the disk diffusion method, and further was verified with the E-test and agar dilution methods. All borderline oxacillin-resistant strains (BORSA) were screened for the mecA gene and virulence factors, including Panton-Valentine leukocidin (PVL). Staphylococcal cassette chromosome mec (SCCmec) typing and spa typing were also carried out.Results: Twelve (4.8%) borderline oxacillin-resistant strains with MIC ≤4 μg/mL were identified. Almost all strains (11/12) were oxacillin-susceptible methicillin resistant S. aureus carrying mecA gene (OS-MRSA). Among the 12 bordeline strains, five spa types (t437, t037, t015, t216, t267) and two SCCmec types (III, IV) were identified, with the most prevalent being t437-SCCmecIV pvl-positive. The second most frequent spa type, t037-SCCmecIII, was sea-positive and did not produce coagulase. The majority of borderline strains originated from skin infections and diabetic foot ulcers and were multidrug-resistant (macrolides, lincosamides and chloramphenicol).Conclusion: This study demonstrated that S. aureus with borderline resistance to oxacillin represented primarily SCCmecIV spa type t437 and coagulase-negative SCCmecIII spa type t037 and were isolated from skin infections and diabetic foot ulcers.Keywords: Staphylococcus aureus, MRSA, OS-MRSA, borderline oxacillin-resistant S. aureus, low-level oxacillin resistance |
| first_indexed | 2024-12-23T20:54:08Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-23T20:54:08Z |
| publishDate | 2019-10-01 |
| publisher | Dove Medical Press |
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| series | Infection and Drug Resistance |
| spelling | doaj.art-9b370f842d7942f186b48a0635a92b9c2022-12-21T17:31:35ZengDove Medical PressInfection and Drug Resistance1178-69732019-10-01Volume 123197320349059Dissemination Of t437-SCCmecIV And Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus aureus Isolated From Skin Infections And Diabetic Foot UlcersStańkowska MGarbacz KPiechowicz LBronk MMaria Stańkowska,1 Katarzyna Garbacz,1 Lidia Piechowicz,2 Marek Bronk3 1Department of Oral Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland; 2Department of Medical Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland; 3Laboratory of Clinical Microbiology, University Clinical Center, Gdansk, PolandCorrespondence: Katarzyna GarbaczDepartment of Oral Microbiology, Medical University of Gdansk, 25 Dębowa St, Gdansk 80-204, PolandTel +48 58 349 1900Fax +48 58 349 1668Email katarzyna.garbacz@gumed.edu.plBackground: In a recent decade, the occurrence of S. aureus isolates with low-level oxacillin resistance, have been reported increasingly. The aim of this study was to estimate the prevalence of S. aureus with low-level of oxacillin resistance and to determine their molecular characteristics, including spa types, SCCmec types and presence of toxin genes.Methods: A total of 249 S. aureus strains were analyzed. Antimicrobial susceptibility was preliminarily tested by the disk diffusion method, and further was verified with the E-test and agar dilution methods. All borderline oxacillin-resistant strains (BORSA) were screened for the mecA gene and virulence factors, including Panton-Valentine leukocidin (PVL). Staphylococcal cassette chromosome mec (SCCmec) typing and spa typing were also carried out.Results: Twelve (4.8%) borderline oxacillin-resistant strains with MIC ≤4 μg/mL were identified. Almost all strains (11/12) were oxacillin-susceptible methicillin resistant S. aureus carrying mecA gene (OS-MRSA). Among the 12 bordeline strains, five spa types (t437, t037, t015, t216, t267) and two SCCmec types (III, IV) were identified, with the most prevalent being t437-SCCmecIV pvl-positive. The second most frequent spa type, t037-SCCmecIII, was sea-positive and did not produce coagulase. The majority of borderline strains originated from skin infections and diabetic foot ulcers and were multidrug-resistant (macrolides, lincosamides and chloramphenicol).Conclusion: This study demonstrated that S. aureus with borderline resistance to oxacillin represented primarily SCCmecIV spa type t437 and coagulase-negative SCCmecIII spa type t037 and were isolated from skin infections and diabetic foot ulcers.Keywords: Staphylococcus aureus, MRSA, OS-MRSA, borderline oxacillin-resistant S. aureus, low-level oxacillin resistance https://www.dovepress.com/dissemination-of-t437-sccmeciv-and-coagulase-negative-t037-sccmeciii-t-peer-reviewed-article-IDRStaphylococcus aureusMRSAOS-MRSAborderline oxacillin-resistant S. aureuslow-level oxacillin resistance |
| spellingShingle | Stańkowska M Garbacz K Piechowicz L Bronk M Dissemination Of t437-SCCmecIV And Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus aureus Isolated From Skin Infections And Diabetic Foot Ulcers Infection and Drug Resistance Staphylococcus aureus MRSA OS-MRSA borderline oxacillin-resistant S. aureus low-level oxacillin resistance |
| title | Dissemination Of t437-SCCmecIV And Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus aureus Isolated From Skin Infections And Diabetic Foot Ulcers |
| title_full | Dissemination Of t437-SCCmecIV And Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus aureus Isolated From Skin Infections And Diabetic Foot Ulcers |
| title_fullStr | Dissemination Of t437-SCCmecIV And Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus aureus Isolated From Skin Infections And Diabetic Foot Ulcers |
| title_full_unstemmed | Dissemination Of t437-SCCmecIV And Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus aureus Isolated From Skin Infections And Diabetic Foot Ulcers |
| title_short | Dissemination Of t437-SCCmecIV And Coagulase-Negative t037-SCCmecIII Types Among Borderline Oxacillin-Resistant Staphylococcus aureus Isolated From Skin Infections And Diabetic Foot Ulcers |
| title_sort | dissemination of t437 sccmeciv and coagulase negative t037 sccmeciii types among borderline oxacillin resistant staphylococcus aureus isolated from skin infections and diabetic foot ulcers |
| topic | Staphylococcus aureus MRSA OS-MRSA borderline oxacillin-resistant S. aureus low-level oxacillin resistance |
| url | https://www.dovepress.com/dissemination-of-t437-sccmeciv-and-coagulase-negative-t037-sccmeciii-t-peer-reviewed-article-IDR |
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