HPLC-DAD Fingerprints Combined With Multivariate Analysis of Epimedii Folium From Major Producing Areas in Eastern Asia: Effect of Geographical Origin and Species
The growth location and plant variety may influence the active components and biological activities of plants used in phytomedicine. In this study, nine sets of different Epimedii Folium, from different representative cultivation locations and Epimedium species, were collected for comparison, using...
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.761551/full |
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| author | Ben Li Marta R. M. Lima Yuhao Nie Long Xu Long Xu Xiang Liu Hongchao Yuan Chen Chen Alberto CP Dias Alberto CP Dias Alberto CP Dias Xiaoying Zhang Xiaoying Zhang Xiaoying Zhang |
| author_facet | Ben Li Marta R. M. Lima Yuhao Nie Long Xu Long Xu Xiang Liu Hongchao Yuan Chen Chen Alberto CP Dias Alberto CP Dias Alberto CP Dias Xiaoying Zhang Xiaoying Zhang Xiaoying Zhang |
| author_sort | Ben Li |
| collection | DOAJ |
| description | The growth location and plant variety may influence the active components and biological activities of plants used in phytomedicine. In this study, nine sets of different Epimedii Folium, from different representative cultivation locations and Epimedium species, were collected for comparison, using HPLC-DAD combined with multivariate analysis. The objective was to investigate the influence of geographical origin and Epimedium species on the quality of Epimedii Folium, and provide applicable guidance for cultivation and quality control of Epimedii Folium. Several Epimedium spp. sets were used to establish the HPLC-DAD fingerprints and 91 peaks (compounds) were selected for the multivariate analysis. Major compounds were analyzed by HPLC-DAD combined with principal component analysis (PCA). HPLC quantitative analysis of known bioactive compounds was performed. Application of PCA to HPLC data showed that Epimedium samples sharing the same geographical origin or species clustered together, indicating that both species and geographical origin have impacts on the quality of Epimedii Folium. The major bioactive flavonoid compounds, epimedin C, icariin and baohuoside I, were identified and quantified. The concentration of bioactive compounds was significantly influenced both by species and geographical origin. E. sagittatum from Sichuan showed the highest content of bioactive compounds. The results showed that both Epimedium species and geographical origin have strong impact into quality of Epimedii Folium. HPLC data combined with multivariate analysis is a suitable approach to inform the selection of cultivation areas and choose Epimedium spp. most suitable for different geographical areas, resulting in improved quality of Epimedii Folium. |
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| language | English |
| last_indexed | 2024-12-14T08:00:26Z |
| publishDate | 2021-11-01 |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-9b374c1c3daa432e8e87c72a9024d9852022-12-21T23:10:26ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-11-011210.3389/fphar.2021.761551761551HPLC-DAD Fingerprints Combined With Multivariate Analysis of Epimedii Folium From Major Producing Areas in Eastern Asia: Effect of Geographical Origin and SpeciesBen Li0Marta R. M. Lima1Yuhao Nie2Long Xu3Long Xu4Xiang Liu5Hongchao Yuan6Chen Chen7Alberto CP Dias8Alberto CP Dias9Alberto CP Dias10Xiaoying Zhang11Xiaoying Zhang12Xiaoying Zhang13Chinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, ChinaDepartment of Agriculture Nutrition and Food Systems, University of New Hampshire, Durham, NH, United StatesChinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, ChinaChinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, ChinaCentre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, PortugalChinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, ChinaJinhuifang Traditional Chinese Medicine Technology Co., Ltd, Hanzhong, ChinaChinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, ChinaChinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, ChinaCentre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, PortugalCentre of Biological Engineering (CEB), University of Minho, Campus de Gualtar, Braga, PortugalChinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, ChinaCentre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, PortugalDepartment of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, CanadaThe growth location and plant variety may influence the active components and biological activities of plants used in phytomedicine. In this study, nine sets of different Epimedii Folium, from different representative cultivation locations and Epimedium species, were collected for comparison, using HPLC-DAD combined with multivariate analysis. The objective was to investigate the influence of geographical origin and Epimedium species on the quality of Epimedii Folium, and provide applicable guidance for cultivation and quality control of Epimedii Folium. Several Epimedium spp. sets were used to establish the HPLC-DAD fingerprints and 91 peaks (compounds) were selected for the multivariate analysis. Major compounds were analyzed by HPLC-DAD combined with principal component analysis (PCA). HPLC quantitative analysis of known bioactive compounds was performed. Application of PCA to HPLC data showed that Epimedium samples sharing the same geographical origin or species clustered together, indicating that both species and geographical origin have impacts on the quality of Epimedii Folium. The major bioactive flavonoid compounds, epimedin C, icariin and baohuoside I, were identified and quantified. The concentration of bioactive compounds was significantly influenced both by species and geographical origin. E. sagittatum from Sichuan showed the highest content of bioactive compounds. The results showed that both Epimedium species and geographical origin have strong impact into quality of Epimedii Folium. HPLC data combined with multivariate analysis is a suitable approach to inform the selection of cultivation areas and choose Epimedium spp. most suitable for different geographical areas, resulting in improved quality of Epimedii Folium.https://www.frontiersin.org/articles/10.3389/fphar.2021.761551/fullEpimedium sphigh performance liquid chromatography (HPLC)principal component analysis (PCA)epimedin Cicariinbaohuoside I |
| spellingShingle | Ben Li Marta R. M. Lima Yuhao Nie Long Xu Long Xu Xiang Liu Hongchao Yuan Chen Chen Alberto CP Dias Alberto CP Dias Alberto CP Dias Xiaoying Zhang Xiaoying Zhang Xiaoying Zhang HPLC-DAD Fingerprints Combined With Multivariate Analysis of Epimedii Folium From Major Producing Areas in Eastern Asia: Effect of Geographical Origin and Species Frontiers in Pharmacology Epimedium sp high performance liquid chromatography (HPLC) principal component analysis (PCA) epimedin C icariin baohuoside I |
| title | HPLC-DAD Fingerprints Combined With Multivariate Analysis of Epimedii Folium From Major Producing Areas in Eastern Asia: Effect of Geographical Origin and Species |
| title_full | HPLC-DAD Fingerprints Combined With Multivariate Analysis of Epimedii Folium From Major Producing Areas in Eastern Asia: Effect of Geographical Origin and Species |
| title_fullStr | HPLC-DAD Fingerprints Combined With Multivariate Analysis of Epimedii Folium From Major Producing Areas in Eastern Asia: Effect of Geographical Origin and Species |
| title_full_unstemmed | HPLC-DAD Fingerprints Combined With Multivariate Analysis of Epimedii Folium From Major Producing Areas in Eastern Asia: Effect of Geographical Origin and Species |
| title_short | HPLC-DAD Fingerprints Combined With Multivariate Analysis of Epimedii Folium From Major Producing Areas in Eastern Asia: Effect of Geographical Origin and Species |
| title_sort | hplc dad fingerprints combined with multivariate analysis of epimedii folium from major producing areas in eastern asia effect of geographical origin and species |
| topic | Epimedium sp high performance liquid chromatography (HPLC) principal component analysis (PCA) epimedin C icariin baohuoside I |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2021.761551/full |
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