Association of IL1 beta gene polymorphism and allograft functions in renal transplant recipients :a case control study from Kashmir Valley
Abstract Background Cytokines have been found to be the important mediators during renal graft outcome. Therefore, we designed this study to investigate the role of recipients’ IL-1 β promoter (−511) and IL-1 β exon-5 (+3954) polymorphisms with the risk of graft outcome. Methodology We enrolled one...
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| Format: | Article |
| Language: | English |
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BMC
2017-03-01
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| Series: | BMC Nephrology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12882-017-0526-5 |
| _version_ | 1819178122929504256 |
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| author | Mohammad Ashraf Bhat Manzoor Ahmad Parry Saniya Nissar Aga Syed Sameer Imtiyaz A. Bhat Zafar A. Shah Roohi Rasool |
| author_facet | Mohammad Ashraf Bhat Manzoor Ahmad Parry Saniya Nissar Aga Syed Sameer Imtiyaz A. Bhat Zafar A. Shah Roohi Rasool |
| author_sort | Mohammad Ashraf Bhat |
| collection | DOAJ |
| description | Abstract Background Cytokines have been found to be the important mediators during renal graft outcome. Therefore, we designed this study to investigate the role of recipients’ IL-1 β promoter (−511) and IL-1 β exon-5 (+3954) polymorphisms with the risk of graft outcome. Methodology We enrolled one hundred recipients of living-related renal transplants together with the age and sex matched controls from the healthy population not having any renal abnormality for this study. Genotype frequencies of the IL-1 β promoter (−511) and IL-1 β exon-5 (+3954) were analyzed using PCR-RFLP technique. Results Our results revealed significant differences in the healthy control group and patient group in IL 1β +3954 (p < 0.001). The frequency of variant type TT genotype was higher in RE group as compared to SGF and showed 4 fold risk of rejection (OR = 4.54, p < 0.069) although p value was not significant. The frequency of wild type CC genotype and CT was not significant (p value 0.89 and 0.74 respectively). Conclusion Our findings suggest that there is a prevalence of mutated allele of IL-1 gene cluster in our population, which may be responsible for renal dysfunction. |
| first_indexed | 2024-12-22T21:37:32Z |
| format | Article |
| id | doaj.art-9b3cf230dbff4047b3902778a60f5cd4 |
| institution | Directory Open Access Journal |
| issn | 1471-2369 |
| language | English |
| last_indexed | 2024-12-22T21:37:32Z |
| publishDate | 2017-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Nephrology |
| spelling | doaj.art-9b3cf230dbff4047b3902778a60f5cd42022-12-21T18:11:41ZengBMCBMC Nephrology1471-23692017-03-011811610.1186/s12882-017-0526-5Association of IL1 beta gene polymorphism and allograft functions in renal transplant recipients :a case control study from Kashmir ValleyMohammad Ashraf Bhat0Manzoor Ahmad Parry1Saniya Nissar2Aga Syed Sameer3Imtiyaz A. Bhat4Zafar A. Shah5Roohi Rasool6Department of Nephrology, Sher-I-Kashmir Institute of Medical SciencesDepartment of Nephrology, Sher-I-Kashmir Institute of Medical SciencesDepartment of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical SciencesDepartment of Basic Medical Sciences, College of Medicine, King Saud bin Abdulaziz University for Health SciencesDepartment of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical SciencesDepartment of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical SciencesDepartment of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical SciencesAbstract Background Cytokines have been found to be the important mediators during renal graft outcome. Therefore, we designed this study to investigate the role of recipients’ IL-1 β promoter (−511) and IL-1 β exon-5 (+3954) polymorphisms with the risk of graft outcome. Methodology We enrolled one hundred recipients of living-related renal transplants together with the age and sex matched controls from the healthy population not having any renal abnormality for this study. Genotype frequencies of the IL-1 β promoter (−511) and IL-1 β exon-5 (+3954) were analyzed using PCR-RFLP technique. Results Our results revealed significant differences in the healthy control group and patient group in IL 1β +3954 (p < 0.001). The frequency of variant type TT genotype was higher in RE group as compared to SGF and showed 4 fold risk of rejection (OR = 4.54, p < 0.069) although p value was not significant. The frequency of wild type CC genotype and CT was not significant (p value 0.89 and 0.74 respectively). Conclusion Our findings suggest that there is a prevalence of mutated allele of IL-1 gene cluster in our population, which may be responsible for renal dysfunction.http://link.springer.com/article/10.1186/s12882-017-0526-5InterleukinsRenal transplantationAllograftEnd stage renal diseaseKashmir |
| spellingShingle | Mohammad Ashraf Bhat Manzoor Ahmad Parry Saniya Nissar Aga Syed Sameer Imtiyaz A. Bhat Zafar A. Shah Roohi Rasool Association of IL1 beta gene polymorphism and allograft functions in renal transplant recipients :a case control study from Kashmir Valley BMC Nephrology Interleukins Renal transplantation Allograft End stage renal disease Kashmir |
| title | Association of IL1 beta gene polymorphism and allograft functions in renal transplant recipients :a case control study from Kashmir Valley |
| title_full | Association of IL1 beta gene polymorphism and allograft functions in renal transplant recipients :a case control study from Kashmir Valley |
| title_fullStr | Association of IL1 beta gene polymorphism and allograft functions in renal transplant recipients :a case control study from Kashmir Valley |
| title_full_unstemmed | Association of IL1 beta gene polymorphism and allograft functions in renal transplant recipients :a case control study from Kashmir Valley |
| title_short | Association of IL1 beta gene polymorphism and allograft functions in renal transplant recipients :a case control study from Kashmir Valley |
| title_sort | association of il1 beta gene polymorphism and allograft functions in renal transplant recipients a case control study from kashmir valley |
| topic | Interleukins Renal transplantation Allograft End stage renal disease Kashmir |
| url | http://link.springer.com/article/10.1186/s12882-017-0526-5 |
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