Investigation of the Therapeutic Effects of Chloroquine in Adriamycin-Induced Hepatotoxicity
The aim of this study is to investigate the therapeutic effects of Chloroquine (CLQ) against Adriamycin (ADR) induced hepatotoxicity. ADR is a chemotherapeutic agent used in the treatment of many cancer types, but it causes hepatotoxicity. CLQ is used as an anti-inflammatory drug in the treatment of...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2021-01-01
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| Series: | The EuroBiotech Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/ebtj-2021-0003 |
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| author | Akin Ali Tuğrul Kaymak Emin Öztürk Emel Karabulut Derya Kuloğlu Nurhan Ceylan Tayfun Toluk Ayşe |
| author_facet | Akin Ali Tuğrul Kaymak Emin Öztürk Emel Karabulut Derya Kuloğlu Nurhan Ceylan Tayfun Toluk Ayşe |
| author_sort | Akin Ali Tuğrul |
| collection | DOAJ |
| description | The aim of this study is to investigate the therapeutic effects of Chloroquine (CLQ) against Adriamycin (ADR) induced hepatotoxicity. ADR is a chemotherapeutic agent used in the treatment of many cancer types, but it causes hepatotoxicity. CLQ is used as an anti-inflammatory drug in the treatment of malaria, rheumatoid arthritis, and pneumonia caused by Covid-19. Rats were divided into four groups: Control group, ADR group (2 mg/kg Adriamycin, one in three days for 30 days, i.p.), CLQ group (50 mg/kg Chloroquine, per day for 30 days, i.p.), ADR+CLQ (2 mg/kg Adriamycin, one in three days for 30 days, i.p. and 50 mg/ kg Chloroquine, per day for 30 days, i.p.). Animals were sacrificed, and liver tissues were extracted for further examinations. Histopathological changes in liver tissues were scored and IL-17 immunostaining was performed to determine the expression levels among experimental groups. Bodyweights in the ADR group decreased significantly compared to the Control group and CLQ group. Furthermore, bodyweight in ADR+CLQ group was significantly higher compared to ADR group. The histopathological score was significantly higher in ADR group when compared to Control and CLQ group while CLQ administrations reduced the damage induced by ADR in the ADR+CLQ group. IL-17 immunoreactivity was considerably increased in the ADR group. On the other hand, IL-17 expressions of ADR+CLQ were substantially less compared to ADR group. We suggest that CLQ can be used as a therapeutic agent to reduce the detrimental effects of ADR, thanks to its anti-inflammatory properties. |
| first_indexed | 2024-12-17T22:48:48Z |
| format | Article |
| id | doaj.art-9b3d8df2496c415ea77a08cc7f3675d3 |
| institution | Directory Open Access Journal |
| issn | 2564-615X |
| language | English |
| last_indexed | 2024-12-17T22:48:48Z |
| publishDate | 2021-01-01 |
| publisher | Sciendo |
| record_format | Article |
| series | The EuroBiotech Journal |
| spelling | doaj.art-9b3d8df2496c415ea77a08cc7f3675d32022-12-21T21:29:43ZengSciendoThe EuroBiotech Journal2564-615X2021-01-015181410.2478/ebtj-2021-0003ebtj-2021-0003Investigation of the Therapeutic Effects of Chloroquine in Adriamycin-Induced HepatotoxicityAkin Ali Tuğrul0Kaymak Emin1Öztürk Emel2Karabulut Derya3Kuloğlu Nurhan4Ceylan Tayfun5Toluk Ayşe6Erciyes University, Science Faculty, Biology Department, Kayseri, TurkeyYozgat Bozok University, Faculty of Medicine, Histology-Embryology Department, Yozgat, TurkeyHarran University, Faculty of Medicine, Histology-Embryology Department, Sanliurfa, TurkeyErciyes University, Faculty of Medicine, Histology-Embryology Department, Kayseri, TurkeyÖmer Halisdemir University, Niğde Zübeyde Hanım Vocational School of Health Services, Aged Care Service, Nigde, TurkeyCappadocia University, Cappadocia Vocational School, Department of Medical Services and Technique, Pathology Laboratory Techniques Pr., Nevsehir, TurkeyErciyes University, Science Faculty, Biology Department, Kayseri, TurkeyThe aim of this study is to investigate the therapeutic effects of Chloroquine (CLQ) against Adriamycin (ADR) induced hepatotoxicity. ADR is a chemotherapeutic agent used in the treatment of many cancer types, but it causes hepatotoxicity. CLQ is used as an anti-inflammatory drug in the treatment of malaria, rheumatoid arthritis, and pneumonia caused by Covid-19. Rats were divided into four groups: Control group, ADR group (2 mg/kg Adriamycin, one in three days for 30 days, i.p.), CLQ group (50 mg/kg Chloroquine, per day for 30 days, i.p.), ADR+CLQ (2 mg/kg Adriamycin, one in three days for 30 days, i.p. and 50 mg/ kg Chloroquine, per day for 30 days, i.p.). Animals were sacrificed, and liver tissues were extracted for further examinations. Histopathological changes in liver tissues were scored and IL-17 immunostaining was performed to determine the expression levels among experimental groups. Bodyweights in the ADR group decreased significantly compared to the Control group and CLQ group. Furthermore, bodyweight in ADR+CLQ group was significantly higher compared to ADR group. The histopathological score was significantly higher in ADR group when compared to Control and CLQ group while CLQ administrations reduced the damage induced by ADR in the ADR+CLQ group. IL-17 immunoreactivity was considerably increased in the ADR group. On the other hand, IL-17 expressions of ADR+CLQ were substantially less compared to ADR group. We suggest that CLQ can be used as a therapeutic agent to reduce the detrimental effects of ADR, thanks to its anti-inflammatory properties.https://doi.org/10.2478/ebtj-2021-0003adriamycinchloroquineil-17liver damage |
| spellingShingle | Akin Ali Tuğrul Kaymak Emin Öztürk Emel Karabulut Derya Kuloğlu Nurhan Ceylan Tayfun Toluk Ayşe Investigation of the Therapeutic Effects of Chloroquine in Adriamycin-Induced Hepatotoxicity The EuroBiotech Journal adriamycin chloroquine il-17 liver damage |
| title | Investigation of the Therapeutic Effects of Chloroquine in Adriamycin-Induced Hepatotoxicity |
| title_full | Investigation of the Therapeutic Effects of Chloroquine in Adriamycin-Induced Hepatotoxicity |
| title_fullStr | Investigation of the Therapeutic Effects of Chloroquine in Adriamycin-Induced Hepatotoxicity |
| title_full_unstemmed | Investigation of the Therapeutic Effects of Chloroquine in Adriamycin-Induced Hepatotoxicity |
| title_short | Investigation of the Therapeutic Effects of Chloroquine in Adriamycin-Induced Hepatotoxicity |
| title_sort | investigation of the therapeutic effects of chloroquine in adriamycin induced hepatotoxicity |
| topic | adriamycin chloroquine il-17 liver damage |
| url | https://doi.org/10.2478/ebtj-2021-0003 |
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