Genome Mining Associated with Analysis of Structure, Antioxidant Activity Reveals the Potential Production of Levan-Rich Exopolysaccharides by Food-Derived <i>Bacillus velezensis</i> VTX20
Exopolysaccharides (EPSs) produced by <i>Bacillus</i> species have recently emerged as promising commercial antioxidants in various industries, such as pharmaceutics and biomedicine. However, little is known about EPS production and function from <i>Bacillus velezensis</i> so...
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MDPI AG
2021-07-01
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| Series: | Applied Sciences |
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| author | Thi Hanh Nguyen Vu Ngoc Tung Quach Ngoc Anh Nguyen Huyen Trang Nguyen Cao Cuong Ngo Tien Dat Nguyen Phu-Ha Ho Ha Hoang Hoang Ha Chu Quyet-Tien Phi |
| author_facet | Thi Hanh Nguyen Vu Ngoc Tung Quach Ngoc Anh Nguyen Huyen Trang Nguyen Cao Cuong Ngo Tien Dat Nguyen Phu-Ha Ho Ha Hoang Hoang Ha Chu Quyet-Tien Phi |
| author_sort | Thi Hanh Nguyen Vu |
| collection | DOAJ |
| description | Exopolysaccharides (EPSs) produced by <i>Bacillus</i> species have recently emerged as promising commercial antioxidants in various industries, such as pharmaceutics and biomedicine. However, little is known about EPS production and function from <i>Bacillus velezensis</i> so far. In the present study, the effect of sugar sources on EPS production by <i>B. velezensis</i> VTX20 and the genetic biosynthesis, characteristics, and antioxidant activity of the resulting EPS were evaluated. The strain VTX20 produced the maximum EPS yield of 75.5 ± 4.8 g/L from an initial 200 g/L of sucrose after a 48-h cultivation. Through genomic analysis, <i>ls</i>-<i>levB</i> operon was found, for the first time, to be responsible for the levan-type EPS production in <i>B. velezensis</i>. Biochemical and structural characterization further confirmed the majority of levan, followed by an extremely low level of dextran biopolymer. The water solubility index and water holding capacity of the EPSs were 81.9 ± 3.4% and 100.2 ± 3.4%, respectively. In vitro antioxidant activity analyses showed strong scavenging activity for 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radical values of 40.1–64.0% and 16.0–40%, respectively. These findings shed light on the EPS biosynthesis of <i>B. velezensis</i> at both structural and genetic levels and the potential application of EPS as a natural antioxidant for pharmaceutical and biomedical industries. |
| first_indexed | 2024-03-10T09:19:02Z |
| format | Article |
| id | doaj.art-9b3f3250d6584935b22589c4490a5108 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T09:19:02Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-9b3f3250d6584935b22589c4490a51082023-11-22T05:23:42ZengMDPI AGApplied Sciences2076-34172021-07-011115705510.3390/app11157055Genome Mining Associated with Analysis of Structure, Antioxidant Activity Reveals the Potential Production of Levan-Rich Exopolysaccharides by Food-Derived <i>Bacillus velezensis</i> VTX20Thi Hanh Nguyen Vu0Ngoc Tung Quach1Ngoc Anh Nguyen2Huyen Trang Nguyen3Cao Cuong Ngo4Tien Dat Nguyen5Phu-Ha Ho6Ha Hoang7Hoang Ha Chu8Quyet-Tien Phi9Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, VietnamVietnam-Russia Tropical Centre, Hanoi 100000, VietnamCenter for Research and Technology Transfer, Vietnam Academy of Science and Technology, Hanoi 100000, VietnamSchool of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, VietnamExopolysaccharides (EPSs) produced by <i>Bacillus</i> species have recently emerged as promising commercial antioxidants in various industries, such as pharmaceutics and biomedicine. However, little is known about EPS production and function from <i>Bacillus velezensis</i> so far. In the present study, the effect of sugar sources on EPS production by <i>B. velezensis</i> VTX20 and the genetic biosynthesis, characteristics, and antioxidant activity of the resulting EPS were evaluated. The strain VTX20 produced the maximum EPS yield of 75.5 ± 4.8 g/L from an initial 200 g/L of sucrose after a 48-h cultivation. Through genomic analysis, <i>ls</i>-<i>levB</i> operon was found, for the first time, to be responsible for the levan-type EPS production in <i>B. velezensis</i>. Biochemical and structural characterization further confirmed the majority of levan, followed by an extremely low level of dextran biopolymer. The water solubility index and water holding capacity of the EPSs were 81.9 ± 3.4% and 100.2 ± 3.4%, respectively. In vitro antioxidant activity analyses showed strong scavenging activity for 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radical values of 40.1–64.0% and 16.0–40%, respectively. These findings shed light on the EPS biosynthesis of <i>B. velezensis</i> at both structural and genetic levels and the potential application of EPS as a natural antioxidant for pharmaceutical and biomedical industries.https://www.mdpi.com/2076-3417/11/15/7055antioxidant activity<i>Bacillus velezensis</i>exopolysaccharides (EPSs)levan |
| spellingShingle | Thi Hanh Nguyen Vu Ngoc Tung Quach Ngoc Anh Nguyen Huyen Trang Nguyen Cao Cuong Ngo Tien Dat Nguyen Phu-Ha Ho Ha Hoang Hoang Ha Chu Quyet-Tien Phi Genome Mining Associated with Analysis of Structure, Antioxidant Activity Reveals the Potential Production of Levan-Rich Exopolysaccharides by Food-Derived <i>Bacillus velezensis</i> VTX20 Applied Sciences antioxidant activity <i>Bacillus velezensis</i> exopolysaccharides (EPSs) levan |
| title | Genome Mining Associated with Analysis of Structure, Antioxidant Activity Reveals the Potential Production of Levan-Rich Exopolysaccharides by Food-Derived <i>Bacillus velezensis</i> VTX20 |
| title_full | Genome Mining Associated with Analysis of Structure, Antioxidant Activity Reveals the Potential Production of Levan-Rich Exopolysaccharides by Food-Derived <i>Bacillus velezensis</i> VTX20 |
| title_fullStr | Genome Mining Associated with Analysis of Structure, Antioxidant Activity Reveals the Potential Production of Levan-Rich Exopolysaccharides by Food-Derived <i>Bacillus velezensis</i> VTX20 |
| title_full_unstemmed | Genome Mining Associated with Analysis of Structure, Antioxidant Activity Reveals the Potential Production of Levan-Rich Exopolysaccharides by Food-Derived <i>Bacillus velezensis</i> VTX20 |
| title_short | Genome Mining Associated with Analysis of Structure, Antioxidant Activity Reveals the Potential Production of Levan-Rich Exopolysaccharides by Food-Derived <i>Bacillus velezensis</i> VTX20 |
| title_sort | genome mining associated with analysis of structure antioxidant activity reveals the potential production of levan rich exopolysaccharides by food derived i bacillus velezensis i vtx20 |
| topic | antioxidant activity <i>Bacillus velezensis</i> exopolysaccharides (EPSs) levan |
| url | https://www.mdpi.com/2076-3417/11/15/7055 |
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