INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL PALM HYBRID MANICORÉ
ABSTRACT Large-scale oil palm propagation (Elaeis guineensis Jacq.) is difficult due to its unique apical meristem. In this context, micropropagation allows the multiplication of seedlings in vitro and the storage of germplasm elites. This study aimed to induce embryogenic calluses from leaves of oi...
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Sociedade de Investigações Florestais
2021-10-01
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| Series: | Revista Árvore |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622021000100238&tlng=en |
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| author | Marlúcia Souza Pádua Vilela Jéssica de Castro e Andrade Raíssa Silveira Santos Vanessa Cristina Stein Patrick Callegari Magnani Santos Alves Luciano Vilela Paiva |
| author_facet | Marlúcia Souza Pádua Vilela Jéssica de Castro e Andrade Raíssa Silveira Santos Vanessa Cristina Stein Patrick Callegari Magnani Santos Alves Luciano Vilela Paiva |
| author_sort | Marlúcia Souza Pádua Vilela |
| collection | DOAJ |
| description | ABSTRACT Large-scale oil palm propagation (Elaeis guineensis Jacq.) is difficult due to its unique apical meristem. In this context, micropropagation allows the multiplication of seedlings in vitro and the storage of germplasm elites. This study aimed to induce embryogenic calluses from leaves of oil palm plants in low concentrations of auxins and to observe the maintenance of these characteristics during in vitro cultivation. Calluses were induced in 0.5 cm leaf explants in Y3 culture medium supplemented with Picloram (4-Amino-3,5,6-trichloro-2-pyridinecarboxylic acid) or 2,4-D (2,4-dichlorophenoxyacetic acid), at concentrations of 0, 1, 3, 6, and 9 mg L-1. The callus with embryogenic appearance was subcultured and evaluated regarding maintenance of embryogenic characteristics by cytochemical analyses. The best treatment for induction of calluses was composed of 1mg.L-1 of Picloram, which led to 30% callus formation. The calluses were classified into4 types, based on color and morphology. The cells of calluses with nodular and beige appearance have embryogenic characteristics, and the embryogenic potential of the cell masses was maintained over the 20 months of cultivation. This differentiated adaptation to the protocol can allow the advance in the mass propagation of oil palm through tissue culture, indicating the importance of investigating the topics proposed by the research. |
| first_indexed | 2024-04-11T16:38:31Z |
| format | Article |
| id | doaj.art-9b3f40bb13fc4925aa2577a748b6d42a |
| institution | Directory Open Access Journal |
| issn | 1806-9088 |
| language | English |
| last_indexed | 2024-04-11T16:38:31Z |
| publishDate | 2021-10-01 |
| publisher | Sociedade de Investigações Florestais |
| record_format | Article |
| series | Revista Árvore |
| spelling | doaj.art-9b3f40bb13fc4925aa2577a748b6d42a2022-12-22T04:13:44ZengSociedade de Investigações FlorestaisRevista Árvore1806-90882021-10-014510.1590/1806-908820210000038INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL PALM HYBRID MANICORÉMarlúcia Souza Pádua Vilelahttps://orcid.org/0000-0002-2657-3662Jéssica de Castro e Andradehttps://orcid.org/0000-0001-8506-7391Raíssa Silveira Santoshttps://orcid.org/0000-0002-8843-8001Vanessa Cristina Steinhttps://orcid.org/0000-0003-1412-0874Patrick Callegari Magnani Santos Alveshttps://orcid.org/0000-0002-0852-1199Luciano Vilela Paivahttps://orcid.org/0000-0002-2577-2168ABSTRACT Large-scale oil palm propagation (Elaeis guineensis Jacq.) is difficult due to its unique apical meristem. In this context, micropropagation allows the multiplication of seedlings in vitro and the storage of germplasm elites. This study aimed to induce embryogenic calluses from leaves of oil palm plants in low concentrations of auxins and to observe the maintenance of these characteristics during in vitro cultivation. Calluses were induced in 0.5 cm leaf explants in Y3 culture medium supplemented with Picloram (4-Amino-3,5,6-trichloro-2-pyridinecarboxylic acid) or 2,4-D (2,4-dichlorophenoxyacetic acid), at concentrations of 0, 1, 3, 6, and 9 mg L-1. The callus with embryogenic appearance was subcultured and evaluated regarding maintenance of embryogenic characteristics by cytochemical analyses. The best treatment for induction of calluses was composed of 1mg.L-1 of Picloram, which led to 30% callus formation. The calluses were classified into4 types, based on color and morphology. The cells of calluses with nodular and beige appearance have embryogenic characteristics, and the embryogenic potential of the cell masses was maintained over the 20 months of cultivation. This differentiated adaptation to the protocol can allow the advance in the mass propagation of oil palm through tissue culture, indicating the importance of investigating the topics proposed by the research.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622021000100238&tlng=enElaeis guineensisMicropropagationCytochemical analysis |
| spellingShingle | Marlúcia Souza Pádua Vilela Jéssica de Castro e Andrade Raíssa Silveira Santos Vanessa Cristina Stein Patrick Callegari Magnani Santos Alves Luciano Vilela Paiva INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL PALM HYBRID MANICORÉ Revista Árvore Elaeis guineensis Micropropagation Cytochemical analysis |
| title | INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL PALM HYBRID MANICORÉ |
| title_full | INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL PALM HYBRID MANICORÉ |
| title_fullStr | INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL PALM HYBRID MANICORÉ |
| title_full_unstemmed | INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL PALM HYBRID MANICORÉ |
| title_short | INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL PALM HYBRID MANICORÉ |
| title_sort | induction and maintenance of embryogenic characteristics of callus of the oil palm hybrid manicore |
| topic | Elaeis guineensis Micropropagation Cytochemical analysis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622021000100238&tlng=en |
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