A Comparative Analysis of MicroRNA Expression in Mild, Moderate, and Severe COVID-19: Insights from Urine, Serum, and Nasopharyngeal Samples
COVID-19, caused by the SARS-CoV-2 virus, manifests with a wide range of clinical symptoms that vary from mild respiratory issues to severe respiratory distress. To effectively manage and predict the outcomes of the disease, it is important to understand the molecular mechanisms underlying its sever...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/12/1681 |
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| author | Raya Soltane Nuha Almulla Ahlam Alasiri Nabila F. Elashmawy Alaa T. Qumsani Fatimah M. Alshehrei Doaa El-Ghareeb Keshek Taha Alqadi Saleh Bakheet AL-Ghamdi Abdou Kamal Allayeh |
| author_facet | Raya Soltane Nuha Almulla Ahlam Alasiri Nabila F. Elashmawy Alaa T. Qumsani Fatimah M. Alshehrei Doaa El-Ghareeb Keshek Taha Alqadi Saleh Bakheet AL-Ghamdi Abdou Kamal Allayeh |
| author_sort | Raya Soltane |
| collection | DOAJ |
| description | COVID-19, caused by the SARS-CoV-2 virus, manifests with a wide range of clinical symptoms that vary from mild respiratory issues to severe respiratory distress. To effectively manage and predict the outcomes of the disease, it is important to understand the molecular mechanisms underlying its severity. This study focuses on analyzing and comparing the expression patterns of microRNAs (miRNAs) in serum, urine, and nasopharyngeal samples from patients with mild, moderate, and severe COVID-19. The aim is to identify potential associations with disease progression and discover suitable markers for diagnosis and prognosis. Our findings indicate the consistent upregulation of miR-21, miR-146a, and miR-155 in urine, serum, and nasopharyngeal samples from patients with mild COVID-19. In moderate cases, there were more significant changes in miRNA expression compared to mild cases. Specifically, miR-let-7 demonstrated upregulation, while miR-146b exhibited downregulation. The most notable alterations in miRNA expression profiles were observed in severe COVID-19 cases, with a significant upregulation of miR-223. Moreover, our analysis using Receiver-operating characteristic (ROC) curves demonstrated that miR-155, miR-let-7, and miR-223 exhibited high sensitivity and specificity, suggesting their potential as biomarkers for distinguishing COVID-19 patients from healthy individuals. Overall, this comparative analysis revealed distinct patterns in miRNA expression. The overlapping expression patterns of miRNAs in urine, serum, and nasopharyngeal samples suggest their potential utility in discriminating disease status. |
| first_indexed | 2024-03-08T20:57:54Z |
| format | Article |
| id | doaj.art-9b3f6a2e3e744e64828e643c008552ee |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-08T20:57:54Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-9b3f6a2e3e744e64828e643c008552ee2023-12-22T13:55:44ZengMDPI AGBiomolecules2218-273X2023-11-011312168110.3390/biom13121681A Comparative Analysis of MicroRNA Expression in Mild, Moderate, and Severe COVID-19: Insights from Urine, Serum, and Nasopharyngeal SamplesRaya Soltane0Nuha Almulla1Ahlam Alasiri2Nabila F. Elashmawy3Alaa T. Qumsani4Fatimah M. Alshehrei5Doaa El-Ghareeb Keshek6Taha Alqadi7Saleh Bakheet AL-Ghamdi8Abdou Kamal Allayeh9Department of Biology, Adham University College, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Biology, Adham University College, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Biology, Adham University College, Umm Al-Qura University, Makkah 21955, Saudi ArabiaBiology Department, College of Science, Jazan University, Jazan 82817, Saudi ArabiaDepartment of Biology, Jumum College University, Umm Al-Qura University, P.O Box 7388, Makkah 21955, Saudi ArabiaDepartment of Biology, Jumum College University, Umm Al-Qura University, P.O Box 7388, Makkah 21955, Saudi ArabiaDepartment of Biology, Jumum College University, Umm Al-Qura University, P.O Box 7388, Makkah 21955, Saudi ArabiaDepartment of Biology, Adham University College, Umm Al-Qura University, Makkah 21955, Saudi ArabiaBiology Department, Faculty of Science, Al-Baha University, Al Baha 65947, Saudi ArabiaVirology Lab 176, Environment and Climate Change Institute, National Research Centre, Giza 12622, EgyptCOVID-19, caused by the SARS-CoV-2 virus, manifests with a wide range of clinical symptoms that vary from mild respiratory issues to severe respiratory distress. To effectively manage and predict the outcomes of the disease, it is important to understand the molecular mechanisms underlying its severity. This study focuses on analyzing and comparing the expression patterns of microRNAs (miRNAs) in serum, urine, and nasopharyngeal samples from patients with mild, moderate, and severe COVID-19. The aim is to identify potential associations with disease progression and discover suitable markers for diagnosis and prognosis. Our findings indicate the consistent upregulation of miR-21, miR-146a, and miR-155 in urine, serum, and nasopharyngeal samples from patients with mild COVID-19. In moderate cases, there were more significant changes in miRNA expression compared to mild cases. Specifically, miR-let-7 demonstrated upregulation, while miR-146b exhibited downregulation. The most notable alterations in miRNA expression profiles were observed in severe COVID-19 cases, with a significant upregulation of miR-223. Moreover, our analysis using Receiver-operating characteristic (ROC) curves demonstrated that miR-155, miR-let-7, and miR-223 exhibited high sensitivity and specificity, suggesting their potential as biomarkers for distinguishing COVID-19 patients from healthy individuals. Overall, this comparative analysis revealed distinct patterns in miRNA expression. The overlapping expression patterns of miRNAs in urine, serum, and nasopharyngeal samples suggest their potential utility in discriminating disease status.https://www.mdpi.com/2218-273X/13/12/1681SARS-CoV-2COVID-19nasopharyngealplasmaurine and MicroRNA expression |
| spellingShingle | Raya Soltane Nuha Almulla Ahlam Alasiri Nabila F. Elashmawy Alaa T. Qumsani Fatimah M. Alshehrei Doaa El-Ghareeb Keshek Taha Alqadi Saleh Bakheet AL-Ghamdi Abdou Kamal Allayeh A Comparative Analysis of MicroRNA Expression in Mild, Moderate, and Severe COVID-19: Insights from Urine, Serum, and Nasopharyngeal Samples Biomolecules SARS-CoV-2 COVID-19 nasopharyngeal plasma urine and MicroRNA expression |
| title | A Comparative Analysis of MicroRNA Expression in Mild, Moderate, and Severe COVID-19: Insights from Urine, Serum, and Nasopharyngeal Samples |
| title_full | A Comparative Analysis of MicroRNA Expression in Mild, Moderate, and Severe COVID-19: Insights from Urine, Serum, and Nasopharyngeal Samples |
| title_fullStr | A Comparative Analysis of MicroRNA Expression in Mild, Moderate, and Severe COVID-19: Insights from Urine, Serum, and Nasopharyngeal Samples |
| title_full_unstemmed | A Comparative Analysis of MicroRNA Expression in Mild, Moderate, and Severe COVID-19: Insights from Urine, Serum, and Nasopharyngeal Samples |
| title_short | A Comparative Analysis of MicroRNA Expression in Mild, Moderate, and Severe COVID-19: Insights from Urine, Serum, and Nasopharyngeal Samples |
| title_sort | comparative analysis of microrna expression in mild moderate and severe covid 19 insights from urine serum and nasopharyngeal samples |
| topic | SARS-CoV-2 COVID-19 nasopharyngeal plasma urine and MicroRNA expression |
| url | https://www.mdpi.com/2218-273X/13/12/1681 |
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