Identification of Staphylococcus aureus Enterotoxin Genes of sea, seb and sec among Healthy Carriers in Ardabil City
Background and Aims: Staphylococcus aureus is a gram-positive, non-motile, non-spore-forming, aerobic, and facultative anaerobic bacterium, which is colonized in anterior part of the human nasal cavity. The carriers' individuals working in the food industry are the main source of spreading food...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Farname
2018-03-01
|
| Series: | Iranian Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://ijmm.ir/article-1-788-en.html |
| _version_ | 1818691761333075968 |
|---|---|
| author | Daryoush Asgarpoor Mahrokh Bahrami Shahrzad Daneshamooz Mehdi Ghasemi |
| author_facet | Daryoush Asgarpoor Mahrokh Bahrami Shahrzad Daneshamooz Mehdi Ghasemi |
| author_sort | Daryoush Asgarpoor |
| collection | DOAJ |
| description | Background and Aims: Staphylococcus aureus is a gram-positive, non-motile, non-spore-forming, aerobic, and facultative anaerobic bacterium, which is colonized in anterior part of the human nasal cavity. The carriers' individuals working in the food industry are the main source of spreading food-borne diseases. Staphylococcal enterotoxins (SEs) are the key virulence factors in the staphylococcal food poisoning (SFP). The aim of this study was to identify the S. aureus enterotoxin genes of sea, seb and sec among healthy carriers.
Materials and Methods: In the current descriptive cross-sectional study, 136 nasal swab samples were collected from the personnel working at butchers, dairy stores, and fast food restaurants through Ardabil city. The samples were cultured and then confirmed using the biochemical tests. The DNA of S. aureus isolates was extracted to detect sea, seb and sec genes as markers for SEA, SEB and SEC enterotoxins using the PCR method.
Results: Among the 136 nasal swab samples, 46 (33.8%) were positive for S. aureus, that were confirmed by the presence of femA gene. Out of 46 isolates, the sea, seb and sec genes were found in 11 (23.9%), 6 (13%) and 5 (10.8%) isolates, respectively.
Conclusions: According to the findings, a significant percentage of the food-chain personnel were nasal carriers of enterotoxigenic S. aureus. Therefore, for prevention and distribution of Staphylococcal infections, screening program and control of such carriers are recommended. |
| first_indexed | 2024-12-17T12:47:02Z |
| format | Article |
| id | doaj.art-9b3fc5fa25d845bd9c4148c1db9e6628 |
| institution | Directory Open Access Journal |
| issn | 1735-8612 2345-4342 |
| language | English |
| last_indexed | 2024-12-17T12:47:02Z |
| publishDate | 2018-03-01 |
| publisher | Farname |
| record_format | Article |
| series | Iranian Journal of Medical Microbiology |
| spelling | doaj.art-9b3fc5fa25d845bd9c4148c1db9e66282022-12-21T21:47:43ZengFarnameIranian Journal of Medical Microbiology1735-86122345-43422018-03-01116149157Identification of Staphylococcus aureus Enterotoxin Genes of sea, seb and sec among Healthy Carriers in Ardabil CityDaryoush Asgarpoor0Mahrokh Bahrami1Shahrzad Daneshamooz2Mehdi Ghasemi3 Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Department of Microbiology, Ardabil Branch, Islamic Azad University, Ardabil, Iran Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Department of Microbiology, Ardabil Branch, Islamic Azad University, Ardabil, Iran Background and Aims: Staphylococcus aureus is a gram-positive, non-motile, non-spore-forming, aerobic, and facultative anaerobic bacterium, which is colonized in anterior part of the human nasal cavity. The carriers' individuals working in the food industry are the main source of spreading food-borne diseases. Staphylococcal enterotoxins (SEs) are the key virulence factors in the staphylococcal food poisoning (SFP). The aim of this study was to identify the S. aureus enterotoxin genes of sea, seb and sec among healthy carriers. Materials and Methods: In the current descriptive cross-sectional study, 136 nasal swab samples were collected from the personnel working at butchers, dairy stores, and fast food restaurants through Ardabil city. The samples were cultured and then confirmed using the biochemical tests. The DNA of S. aureus isolates was extracted to detect sea, seb and sec genes as markers for SEA, SEB and SEC enterotoxins using the PCR method. Results: Among the 136 nasal swab samples, 46 (33.8%) were positive for S. aureus, that were confirmed by the presence of femA gene. Out of 46 isolates, the sea, seb and sec genes were found in 11 (23.9%), 6 (13%) and 5 (10.8%) isolates, respectively. Conclusions: According to the findings, a significant percentage of the food-chain personnel were nasal carriers of enterotoxigenic S. aureus. Therefore, for prevention and distribution of Staphylococcal infections, screening program and control of such carriers are recommended.http://ijmm.ir/article-1-788-en.htmlStaphylococcus aureusEnterotoxinPCR |
| spellingShingle | Daryoush Asgarpoor Mahrokh Bahrami Shahrzad Daneshamooz Mehdi Ghasemi Identification of Staphylococcus aureus Enterotoxin Genes of sea, seb and sec among Healthy Carriers in Ardabil City Iranian Journal of Medical Microbiology Staphylococcus aureus Enterotoxin PCR |
| title | Identification of Staphylococcus aureus Enterotoxin Genes of sea, seb and sec among Healthy Carriers in Ardabil City |
| title_full | Identification of Staphylococcus aureus Enterotoxin Genes of sea, seb and sec among Healthy Carriers in Ardabil City |
| title_fullStr | Identification of Staphylococcus aureus Enterotoxin Genes of sea, seb and sec among Healthy Carriers in Ardabil City |
| title_full_unstemmed | Identification of Staphylococcus aureus Enterotoxin Genes of sea, seb and sec among Healthy Carriers in Ardabil City |
| title_short | Identification of Staphylococcus aureus Enterotoxin Genes of sea, seb and sec among Healthy Carriers in Ardabil City |
| title_sort | identification of staphylococcus aureus enterotoxin genes of sea seb and sec among healthy carriers in ardabil city |
| topic | Staphylococcus aureus Enterotoxin PCR |
| url | http://ijmm.ir/article-1-788-en.html |
| work_keys_str_mv | AT daryoushasgarpoor identificationofstaphylococcusaureusenterotoxingenesofseasebandsecamonghealthycarriersinardabilcity AT mahrokhbahrami identificationofstaphylococcusaureusenterotoxingenesofseasebandsecamonghealthycarriersinardabilcity AT shahrzaddaneshamooz identificationofstaphylococcusaureusenterotoxingenesofseasebandsecamonghealthycarriersinardabilcity AT mehdighasemi identificationofstaphylococcusaureusenterotoxingenesofseasebandsecamonghealthycarriersinardabilcity |