Oxidative Stress Response’s Kinetics after 60 Minutes at Different (30% or 100%) Normobaric Hyperoxia Exposures
Oxygen is a powerful trigger for cellular reactions and is used in many pathologies, including oxidative stress. However, the effects of oxygen over time and at different partial pressures remain poorly understood. In this study, the metabolic responses of normobaric oxygen intake for 1 h to mild (3...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/1/664 |
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| author | Clément Leveque Simona Mrakic-Sposta Pierre Lafère Alessandra Vezzoli Peter Germonpré Alexandre Beer Stéphane Mievis Fabio Virgili Kate Lambrechts Sigrid Theunissen François Guerrero Costantino Balestra |
| author_facet | Clément Leveque Simona Mrakic-Sposta Pierre Lafère Alessandra Vezzoli Peter Germonpré Alexandre Beer Stéphane Mievis Fabio Virgili Kate Lambrechts Sigrid Theunissen François Guerrero Costantino Balestra |
| author_sort | Clément Leveque |
| collection | DOAJ |
| description | Oxygen is a powerful trigger for cellular reactions and is used in many pathologies, including oxidative stress. However, the effects of oxygen over time and at different partial pressures remain poorly understood. In this study, the metabolic responses of normobaric oxygen intake for 1 h to mild (30%) and high (100%) inspired fractions were investigated. Fourteen healthy non-smoking subjects (7 males and 7 females; age: 29.9 ± 11.1 years, height: 168.2 ± 9.37 cm; weight: 64.4 ± 12.3 kg; BMI: 22.7 ± 4.1) were randomly assigned in the two groups. Blood samples were taken before the intake at 30 min, 2 h, 8 h, 24 h, and 48 h after the single oxygen exposure. The level of oxidation was evaluated by the rate of reactive oxygen species (ROS) and the levels of isoprostane. Antioxidant reactions were observed by total antioxidant capacity (TAC), superoxide dismutase (SOD), and catalase (CAT). The inflammatory response was measured using interleukin-6 (IL-6), neopterin, creatinine, and urates. Oxidation markers increased from 30 min on to reach a peak at 8 h. From 8 h post intake, the markers of inflammation took over, and more significantly with 100% than with 30%. This study suggests a biphasic response over time characterized by an initial “permissive oxidation” followed by increased inflammation. The antioxidant protection system seems not to be the leading actor in the first place. The kinetics of enzymatic reactions need to be better studied to establish therapeutic, training, or rehabilitation protocols aiming at a more targeted use of oxygen. |
| first_indexed | 2024-03-11T09:59:05Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:59:05Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9b40ab66ada8408b849df572c642da072023-11-16T15:36:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0124166410.3390/ijms24010664Oxidative Stress Response’s Kinetics after 60 Minutes at Different (30% or 100%) Normobaric Hyperoxia ExposuresClément Leveque0Simona Mrakic-Sposta1Pierre Lafère2Alessandra Vezzoli3Peter Germonpré4Alexandre Beer5Stéphane Mievis6Fabio Virgili7Kate Lambrechts8Sigrid Theunissen9François Guerrero10Costantino Balestra11Environmental, Occupational, Aging (Integrative) Physiology Laboratory, Haute Ecole Bruxelles-Brabant (HE2B), 1160 Brussels, BelgiumInstitute of Clinical Physiology, National Research Council (CNR), 20162 Milan, ItalyEnvironmental, Occupational, Aging (Integrative) Physiology Laboratory, Haute Ecole Bruxelles-Brabant (HE2B), 1160 Brussels, BelgiumInstitute of Clinical Physiology, National Research Council (CNR), 20162 Milan, ItalyEnvironmental, Occupational, Aging (Integrative) Physiology Laboratory, Haute Ecole Bruxelles-Brabant (HE2B), 1160 Brussels, BelgiumEnvironmental, Occupational, Aging (Integrative) Physiology Laboratory, Haute Ecole Bruxelles-Brabant (HE2B), 1160 Brussels, BelgiumEnvironmental, Occupational, Aging (Integrative) Physiology Laboratory, Haute Ecole Bruxelles-Brabant (HE2B), 1160 Brussels, BelgiumCouncil for Agricultural Research and Economics-Food and Nutrition Research Centre (C.R.E.A.-AN), Rome, ItalyEnvironmental, Occupational, Aging (Integrative) Physiology Laboratory, Haute Ecole Bruxelles-Brabant (HE2B), 1160 Brussels, BelgiumEnvironmental, Occupational, Aging (Integrative) Physiology Laboratory, Haute Ecole Bruxelles-Brabant (HE2B), 1160 Brussels, BelgiumLaboratoire ORPHY, EA 4324, Université de Bretagne Occidentale, 29238 Brest, FranceEnvironmental, Occupational, Aging (Integrative) Physiology Laboratory, Haute Ecole Bruxelles-Brabant (HE2B), 1160 Brussels, BelgiumOxygen is a powerful trigger for cellular reactions and is used in many pathologies, including oxidative stress. However, the effects of oxygen over time and at different partial pressures remain poorly understood. In this study, the metabolic responses of normobaric oxygen intake for 1 h to mild (30%) and high (100%) inspired fractions were investigated. Fourteen healthy non-smoking subjects (7 males and 7 females; age: 29.9 ± 11.1 years, height: 168.2 ± 9.37 cm; weight: 64.4 ± 12.3 kg; BMI: 22.7 ± 4.1) were randomly assigned in the two groups. Blood samples were taken before the intake at 30 min, 2 h, 8 h, 24 h, and 48 h after the single oxygen exposure. The level of oxidation was evaluated by the rate of reactive oxygen species (ROS) and the levels of isoprostane. Antioxidant reactions were observed by total antioxidant capacity (TAC), superoxide dismutase (SOD), and catalase (CAT). The inflammatory response was measured using interleukin-6 (IL-6), neopterin, creatinine, and urates. Oxidation markers increased from 30 min on to reach a peak at 8 h. From 8 h post intake, the markers of inflammation took over, and more significantly with 100% than with 30%. This study suggests a biphasic response over time characterized by an initial “permissive oxidation” followed by increased inflammation. The antioxidant protection system seems not to be the leading actor in the first place. The kinetics of enzymatic reactions need to be better studied to establish therapeutic, training, or rehabilitation protocols aiming at a more targeted use of oxygen.https://www.mdpi.com/1422-0067/24/1/664normobaric oxygen paradoxhyperoxic–hypoxic paradoxhyperoxiaoxygen biologycellular reactionshuman |
| spellingShingle | Clément Leveque Simona Mrakic-Sposta Pierre Lafère Alessandra Vezzoli Peter Germonpré Alexandre Beer Stéphane Mievis Fabio Virgili Kate Lambrechts Sigrid Theunissen François Guerrero Costantino Balestra Oxidative Stress Response’s Kinetics after 60 Minutes at Different (30% or 100%) Normobaric Hyperoxia Exposures International Journal of Molecular Sciences normobaric oxygen paradox hyperoxic–hypoxic paradox hyperoxia oxygen biology cellular reactions human |
| title | Oxidative Stress Response’s Kinetics after 60 Minutes at Different (30% or 100%) Normobaric Hyperoxia Exposures |
| title_full | Oxidative Stress Response’s Kinetics after 60 Minutes at Different (30% or 100%) Normobaric Hyperoxia Exposures |
| title_fullStr | Oxidative Stress Response’s Kinetics after 60 Minutes at Different (30% or 100%) Normobaric Hyperoxia Exposures |
| title_full_unstemmed | Oxidative Stress Response’s Kinetics after 60 Minutes at Different (30% or 100%) Normobaric Hyperoxia Exposures |
| title_short | Oxidative Stress Response’s Kinetics after 60 Minutes at Different (30% or 100%) Normobaric Hyperoxia Exposures |
| title_sort | oxidative stress response s kinetics after 60 minutes at different 30 or 100 normobaric hyperoxia exposures |
| topic | normobaric oxygen paradox hyperoxic–hypoxic paradox hyperoxia oxygen biology cellular reactions human |
| url | https://www.mdpi.com/1422-0067/24/1/664 |
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