Standard Assays for the Study of Autophagy in the Ex Vivo Retina
Autophagy is a catabolic pathway that mediates the degradation and recycling of intracellular components, and is a key player in a variety of physiological processes in cells and tissues. Recent studies of autophagy in the eye suggest that this pathway is fundamental for the preservation of retinal...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-10-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/6/4/37 |
| _version_ | 1797727714855092224 |
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| author | Raquel Gómez-Sintes Beatriz Villarejo-Zori Ana Serrano-Puebla Lorena Esteban-Martínez Elena Sierra-Filardi Ignacio Ramírez-Pardo Natalia Rodríguez-Muela Patricia Boya |
| author_facet | Raquel Gómez-Sintes Beatriz Villarejo-Zori Ana Serrano-Puebla Lorena Esteban-Martínez Elena Sierra-Filardi Ignacio Ramírez-Pardo Natalia Rodríguez-Muela Patricia Boya |
| author_sort | Raquel Gómez-Sintes |
| collection | DOAJ |
| description | Autophagy is a catabolic pathway that mediates the degradation and recycling of intracellular components, and is a key player in a variety of physiological processes in cells and tissues. Recent studies of autophagy in the eye suggest that this pathway is fundamental for the preservation of retinal homeostasis. Given its accessible location outside the brain, the retina is an ideal organ in which to study the central nervous system and a wide range of neuronal processes, from development to neurodegeneration. Here we review several methods used to assess autophagy in the retina in both physiological and pathological conditions. |
| first_indexed | 2024-03-12T11:03:40Z |
| format | Article |
| id | doaj.art-9b42ac10fe0e4d7e8500eeb393415ab3 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T11:03:40Z |
| publishDate | 2017-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-9b42ac10fe0e4d7e8500eeb393415ab32023-09-02T04:26:01ZengMDPI AGCells2073-44092017-10-01643710.3390/cells6040037cells6040037Standard Assays for the Study of Autophagy in the Ex Vivo RetinaRaquel Gómez-Sintes0Beatriz Villarejo-Zori1Ana Serrano-Puebla2Lorena Esteban-Martínez3Elena Sierra-Filardi4Ignacio Ramírez-Pardo5Natalia Rodríguez-Muela6Patricia Boya7Departament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, SpainDepartament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, SpainDepartament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, SpainDepartament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, SpainDepartament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, SpainDepartament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, SpainDepartament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, SpainDepartament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, SpainAutophagy is a catabolic pathway that mediates the degradation and recycling of intracellular components, and is a key player in a variety of physiological processes in cells and tissues. Recent studies of autophagy in the eye suggest that this pathway is fundamental for the preservation of retinal homeostasis. Given its accessible location outside the brain, the retina is an ideal organ in which to study the central nervous system and a wide range of neuronal processes, from development to neurodegeneration. Here we review several methods used to assess autophagy in the retina in both physiological and pathological conditions.https://www.mdpi.com/2073-4409/6/4/37autophagyretinaorganotypic cultureslysosomesmitophagy |
| spellingShingle | Raquel Gómez-Sintes Beatriz Villarejo-Zori Ana Serrano-Puebla Lorena Esteban-Martínez Elena Sierra-Filardi Ignacio Ramírez-Pardo Natalia Rodríguez-Muela Patricia Boya Standard Assays for the Study of Autophagy in the Ex Vivo Retina Cells autophagy retina organotypic cultures lysosomes mitophagy |
| title | Standard Assays for the Study of Autophagy in the Ex Vivo Retina |
| title_full | Standard Assays for the Study of Autophagy in the Ex Vivo Retina |
| title_fullStr | Standard Assays for the Study of Autophagy in the Ex Vivo Retina |
| title_full_unstemmed | Standard Assays for the Study of Autophagy in the Ex Vivo Retina |
| title_short | Standard Assays for the Study of Autophagy in the Ex Vivo Retina |
| title_sort | standard assays for the study of autophagy in the ex vivo retina |
| topic | autophagy retina organotypic cultures lysosomes mitophagy |
| url | https://www.mdpi.com/2073-4409/6/4/37 |
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