Bovine tuberculosis in the Republic of Macedonia: postmortem, microbiological and molecular study in slaughtered reactor cattle
Bovine tuberculosis is a chronic infectious disease in cattle caused mainly by Mycobacterium bovis and to a lesser extent by Mycobacterium caprae. The other members of the Mycobacterium tuberculosis complex (MTBC) can also cause the disease in domestic and wild animals and all of them have a zoonoti...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Ss.Cyril and Methodius University, Skopje
2017-03-01
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| Series: | Macedonian Veterinary Review |
| Subjects: | |
| Online Access: | http://www.macvetrev.mk/2017-1/macvetrev-2016-0097.pdf |
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| author | Iskra Cvetkovik Slavcho Mrenoshki Kiril Krstevski Igor Djadjovski Branko Angjelovski Zagorka Popova Aleksandar Janevski Aleksandar Dodovski Aleksandar Cvetkovikj |
| author_facet | Iskra Cvetkovik Slavcho Mrenoshki Kiril Krstevski Igor Djadjovski Branko Angjelovski Zagorka Popova Aleksandar Janevski Aleksandar Dodovski Aleksandar Cvetkovikj |
| author_sort | Iskra Cvetkovik |
| collection | DOAJ |
| description | Bovine tuberculosis is a chronic infectious disease in cattle caused mainly by Mycobacterium bovis and to a lesser extent by Mycobacterium caprae. The other members of the Mycobacterium tuberculosis complex (MTBC) can also cause the disease in domestic and wild animals and all of them have a zoonotic potential. The main purpose of the study was to determine the presence and distribution of the tuberculous lesions in reactor cattle, and to isolate and identify the causative agents of bovine tuberculosis in the Republic of Macedonia. Lymph nodes and affected organs from 188 reactor cattle slaughtered due to a positive intradermal comparative cervical tuberculin test were analyzed by detection of tuberculous lesions, followed by isolation and molecular identification of the isolated mycobacteria. The isolation was performed on selective media – Lowenstein Jensen with glycerol, Lowenstein Jensen without glycerol and Stonebrink medium supplemented with pyruvate. The molecular identification of the MTBC members was performed by analysis of the Regions of difference (RD1, RD9 and RD4) and detection of single nucleotide polymorphisms in the lepA gene for Mycobacterium caprae. Typical tuberculous lesions were detected in 62 animals (33.0%) and the lesions were most prevalent in the mediastinal lymph nodes (47.5%). The isolated mycobacteria in the MTBC were identified as Mycobacterium bovis and Mycobacterium caprae and were found in both animals with visible lesions (82.2%) and animals without visible lesions (27.7%). The slaughterhouse postmortem examinations and laboratory investigations should be included on regular bases in order to improve the National eradication program. |
| first_indexed | 2024-04-13T01:37:46Z |
| format | Article |
| id | doaj.art-9b486df78c0d4cb7b38f7ddaa82a98f7 |
| institution | Directory Open Access Journal |
| issn | 1409-7621 1857-7415 |
| language | English |
| last_indexed | 2024-04-13T01:37:46Z |
| publishDate | 2017-03-01 |
| publisher | Ss.Cyril and Methodius University, Skopje |
| record_format | Article |
| series | Macedonian Veterinary Review |
| spelling | doaj.art-9b486df78c0d4cb7b38f7ddaa82a98f72022-12-22T03:08:19ZengSs.Cyril and Methodius University, SkopjeMacedonian Veterinary Review1409-76211857-74152017-03-01401435210.1515/macvetrev-2016-0097Bovine tuberculosis in the Republic of Macedonia: postmortem, microbiological and molecular study in slaughtered reactor cattleIskra Cvetkovik0Slavcho Mrenoshki1Kiril Krstevski2Igor Djadjovski3Branko Angjelovski4Zagorka Popova5Aleksandar Janevski6Aleksandar Dodovski7Aleksandar Cvetkovikj8Veterinary Institute, Faculty of Veterinary Medicine, Ss. Cyril and MethodiusUniversity in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of MacedoniaVeterinary Institute, Faculty of Veterinary Medicine, Ss. Cyril and MethodiusUniversity in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of MacedoniaVeterinary Institute, Faculty of Veterinary Medicine, Ss. Cyril and MethodiusUniversity in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of MacedoniaVeterinary Institute, Faculty of Veterinary Medicine, Ss. Cyril and MethodiusUniversity in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of MacedoniaVeterinary Institute, Faculty of Veterinary Medicine, Ss. Cyril and MethodiusUniversity in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of MacedoniaVeterinary Institute, Faculty of Veterinary Medicine, Ss. Cyril and MethodiusUniversity in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of MacedoniaVeterinary Institute, Faculty of Veterinary Medicine, Ss. Cyril and MethodiusUniversity in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of MacedoniaVeterinary Institute, Faculty of Veterinary Medicine, Ss. Cyril and MethodiusUniversity in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of MacedoniaVeterinary Institute, Faculty of Veterinary Medicine, Ss. Cyril and MethodiusUniversity in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of MacedoniaBovine tuberculosis is a chronic infectious disease in cattle caused mainly by Mycobacterium bovis and to a lesser extent by Mycobacterium caprae. The other members of the Mycobacterium tuberculosis complex (MTBC) can also cause the disease in domestic and wild animals and all of them have a zoonotic potential. The main purpose of the study was to determine the presence and distribution of the tuberculous lesions in reactor cattle, and to isolate and identify the causative agents of bovine tuberculosis in the Republic of Macedonia. Lymph nodes and affected organs from 188 reactor cattle slaughtered due to a positive intradermal comparative cervical tuberculin test were analyzed by detection of tuberculous lesions, followed by isolation and molecular identification of the isolated mycobacteria. The isolation was performed on selective media – Lowenstein Jensen with glycerol, Lowenstein Jensen without glycerol and Stonebrink medium supplemented with pyruvate. The molecular identification of the MTBC members was performed by analysis of the Regions of difference (RD1, RD9 and RD4) and detection of single nucleotide polymorphisms in the lepA gene for Mycobacterium caprae. Typical tuberculous lesions were detected in 62 animals (33.0%) and the lesions were most prevalent in the mediastinal lymph nodes (47.5%). The isolated mycobacteria in the MTBC were identified as Mycobacterium bovis and Mycobacterium caprae and were found in both animals with visible lesions (82.2%) and animals without visible lesions (27.7%). The slaughterhouse postmortem examinations and laboratory investigations should be included on regular bases in order to improve the National eradication program.http://www.macvetrev.mk/2017-1/macvetrev-2016-0097.pdfbovine tuberculosisMycobacterium bovisMycobacterium capraePCRRepublic of Macedonia |
| spellingShingle | Iskra Cvetkovik Slavcho Mrenoshki Kiril Krstevski Igor Djadjovski Branko Angjelovski Zagorka Popova Aleksandar Janevski Aleksandar Dodovski Aleksandar Cvetkovikj Bovine tuberculosis in the Republic of Macedonia: postmortem, microbiological and molecular study in slaughtered reactor cattle Macedonian Veterinary Review bovine tuberculosis Mycobacterium bovis Mycobacterium caprae PCR Republic of Macedonia |
| title | Bovine tuberculosis in the Republic of Macedonia: postmortem, microbiological and molecular study in slaughtered reactor cattle |
| title_full | Bovine tuberculosis in the Republic of Macedonia: postmortem, microbiological and molecular study in slaughtered reactor cattle |
| title_fullStr | Bovine tuberculosis in the Republic of Macedonia: postmortem, microbiological and molecular study in slaughtered reactor cattle |
| title_full_unstemmed | Bovine tuberculosis in the Republic of Macedonia: postmortem, microbiological and molecular study in slaughtered reactor cattle |
| title_short | Bovine tuberculosis in the Republic of Macedonia: postmortem, microbiological and molecular study in slaughtered reactor cattle |
| title_sort | bovine tuberculosis in the republic of macedonia postmortem microbiological and molecular study in slaughtered reactor cattle |
| topic | bovine tuberculosis Mycobacterium bovis Mycobacterium caprae PCR Republic of Macedonia |
| url | http://www.macvetrev.mk/2017-1/macvetrev-2016-0097.pdf |
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