Ethephon-induced phenylpropanoid accumulation and related gene expression in tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) hairy root
The main flavonoid compound rutin is highly expressed in the crop plant buckwheat (Fagopyrum tataricum (L.) Gaertn.) and plays important roles in the human diet. In this study, phenylpropanoid production in buckwheat hairy roots was evaluated following ethephon treatment. Using quantitative real-tim...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-03-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2017.1282835 |
| _version_ | 1818935796316504064 |
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| author | Xiaohua Li Aye Aye Thwe Chang Ha Park Sun Ju Kim Mariadhas Valan Arasu Naif Abdullah Al-Dhabi Sook Young Lee Sang Un Park |
| author_facet | Xiaohua Li Aye Aye Thwe Chang Ha Park Sun Ju Kim Mariadhas Valan Arasu Naif Abdullah Al-Dhabi Sook Young Lee Sang Un Park |
| author_sort | Xiaohua Li |
| collection | DOAJ |
| description | The main flavonoid compound rutin is highly expressed in the crop plant buckwheat (Fagopyrum tataricum (L.) Gaertn.) and plays important roles in the human diet. In this study, phenylpropanoid production in buckwheat hairy roots was evaluated following ethephon treatment. Using quantitative real-time polymerase chain reaction and high-performance liquid chromatography, we analysed the relationship between flavonoid and anthocyanin biosynthetic pathway gene regulation and the relative accumulation of the secondary compounds in ethephon-treated buckwheat hairy roots. Generally, the transcription of the biosynthetic pathway genes varied between the treated samples and controls. Most of the flavonoid biosynthetic genes were upregulated by ethephon, typically after four days of treatment. The application of 0.5 mg/L ethephon markedly induced anthocyanin production in hairy roots compared to that induced by the other concentrations tested (0, 1 and 2 mg/L). These data indicate that anthocyanin biosynthesis may play an important role in the response of buckwheat to ethephon-induced stress. |
| first_indexed | 2024-12-20T05:25:52Z |
| format | Article |
| id | doaj.art-9b48dc6a0ee2413983aec8463a1fdc23 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-20T05:25:52Z |
| publishDate | 2017-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-9b48dc6a0ee2413983aec8463a1fdc232022-12-21T19:51:52ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302017-03-0131230431110.1080/13102818.2017.12828351282835Ethephon-induced phenylpropanoid accumulation and related gene expression in tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) hairy rootXiaohua Li0Aye Aye Thwe1Chang Ha Park2Sun Ju Kim3Mariadhas Valan Arasu4Naif Abdullah Al-Dhabi5Sook Young Lee6Sang Un Park7Chungnam National UniversityChungnam National UniversityChungnam National UniversityChungnam National UniversityKing Saud UniversityKing Saud UniversityChosun UniversityChungnam National UniversityThe main flavonoid compound rutin is highly expressed in the crop plant buckwheat (Fagopyrum tataricum (L.) Gaertn.) and plays important roles in the human diet. In this study, phenylpropanoid production in buckwheat hairy roots was evaluated following ethephon treatment. Using quantitative real-time polymerase chain reaction and high-performance liquid chromatography, we analysed the relationship between flavonoid and anthocyanin biosynthetic pathway gene regulation and the relative accumulation of the secondary compounds in ethephon-treated buckwheat hairy roots. Generally, the transcription of the biosynthetic pathway genes varied between the treated samples and controls. Most of the flavonoid biosynthetic genes were upregulated by ethephon, typically after four days of treatment. The application of 0.5 mg/L ethephon markedly induced anthocyanin production in hairy roots compared to that induced by the other concentrations tested (0, 1 and 2 mg/L). These data indicate that anthocyanin biosynthesis may play an important role in the response of buckwheat to ethephon-induced stress.http://dx.doi.org/10.1080/13102818.2017.1282835Ethephonflavonol biosynthesisgene expressionhairy roottartary buckwheat |
| spellingShingle | Xiaohua Li Aye Aye Thwe Chang Ha Park Sun Ju Kim Mariadhas Valan Arasu Naif Abdullah Al-Dhabi Sook Young Lee Sang Un Park Ethephon-induced phenylpropanoid accumulation and related gene expression in tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) hairy root Biotechnology & Biotechnological Equipment Ethephon flavonol biosynthesis gene expression hairy root tartary buckwheat |
| title | Ethephon-induced phenylpropanoid accumulation and related gene expression in tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) hairy root |
| title_full | Ethephon-induced phenylpropanoid accumulation and related gene expression in tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) hairy root |
| title_fullStr | Ethephon-induced phenylpropanoid accumulation and related gene expression in tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) hairy root |
| title_full_unstemmed | Ethephon-induced phenylpropanoid accumulation and related gene expression in tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) hairy root |
| title_short | Ethephon-induced phenylpropanoid accumulation and related gene expression in tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) hairy root |
| title_sort | ethephon induced phenylpropanoid accumulation and related gene expression in tartary buckwheat fagopyrum tataricum l gaertn hairy root |
| topic | Ethephon flavonol biosynthesis gene expression hairy root tartary buckwheat |
| url | http://dx.doi.org/10.1080/13102818.2017.1282835 |
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