Antibacterial activity testing methods for hydrophobic patterned surfaces
Abstract One strategy to decrease the incidence of hospital-acquired infections is to avoid the survival of pathogens in the environment by the development of surfaces with antimicrobial activity. To study the antibacterial behaviour of active surfaces, different approaches have been developed of wh...
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-85995-9 |
| _version_ | 1818979012528046080 |
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| author | Ana Perez-Gavilan Joana Vieira de Castro Ainara Arana Santos Merino Aritz Retolaza Sofia A. Alves Achille Francone Nikolaos Kehagias Clivia M. Sotomayor-Torres Donato Cocina Renato Mortera Salvatore Crapanzano Carlos Javier Pelegrín María Carmen Garrigos Alfonso Jiménez Begoña Galindo Mari Carmen Araque Donna Dykeman Nuno M. Neves Jose Maria Marimón |
| author_facet | Ana Perez-Gavilan Joana Vieira de Castro Ainara Arana Santos Merino Aritz Retolaza Sofia A. Alves Achille Francone Nikolaos Kehagias Clivia M. Sotomayor-Torres Donato Cocina Renato Mortera Salvatore Crapanzano Carlos Javier Pelegrín María Carmen Garrigos Alfonso Jiménez Begoña Galindo Mari Carmen Araque Donna Dykeman Nuno M. Neves Jose Maria Marimón |
| author_sort | Ana Perez-Gavilan |
| collection | DOAJ |
| description | Abstract One strategy to decrease the incidence of hospital-acquired infections is to avoid the survival of pathogens in the environment by the development of surfaces with antimicrobial activity. To study the antibacterial behaviour of active surfaces, different approaches have been developed of which ISO 22916 is the standard. To assess the performance of different testing methodologies to analyse the antibacterial activity of hydrophobic surface patterned plastics as part of a Horizon 2020 European research project. Four different testing methods were used to study the antibacterial activity of a patterned film, including the ISO 22916 standard, the immersion method, the touch-transfer inoculation method, and the swab inoculation method, this latter developed specifically for this project. The non-realistic test conditions of the ISO 22916 standard showed this method to be non-appropriate in the study of hydrophobic patterned surfaces. The immersion method also showed no differences between patterned films and smooth controls due to the lack of attachment of testing bacteria on both surfaces. The antibacterial activity of films could be demonstrated by the touch-transfer and the swab inoculation methods, that more precisely mimicked the way of high-touch surfaces contamination, and showed to be the best methodologies to test the antibacterial activity of patterned hydrophobic surfaces. A new ISO standard would be desirable as the reference method to study the antibacterial behaviour of patterned surfaces. |
| first_indexed | 2024-12-20T16:52:46Z |
| format | Article |
| id | doaj.art-9b49e2ef7e5f4f3898b48d782921b5b2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T16:52:46Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9b49e2ef7e5f4f3898b48d782921b5b22022-12-21T19:32:48ZengNature PortfolioScientific Reports2045-23222021-03-0111111010.1038/s41598-021-85995-9Antibacterial activity testing methods for hydrophobic patterned surfacesAna Perez-Gavilan0Joana Vieira de Castro1Ainara Arana2Santos Merino3Aritz Retolaza4Sofia A. Alves5Achille Francone6Nikolaos Kehagias7Clivia M. Sotomayor-Torres8Donato Cocina9Renato Mortera10Salvatore Crapanzano11Carlos Javier Pelegrín12María Carmen Garrigos13Alfonso Jiménez14Begoña Galindo15Mari Carmen Araque16Donna Dykeman17Nuno M. Neves18Jose Maria Marimón19Biodonostia, Infectious Diseases Area, Respiratory Infection and Antimicrobial Resistance Group, Microbiology Department, Osakidetza Basque Health Service, Donostialdea Integrated Health Organisation3B’s Research Group, I3Bs—Research Institute On Biomaterials, Headquarters of the European Institute of Excellence On Tissue Engineering and Regenerative Medicine, Biodegradables and Biomimetics of University of MinhoBiodonostia, Infectious Diseases Area, Respiratory Infection and Antimicrobial Resistance Group, Microbiology Department, Osakidetza Basque Health Service, Donostialdea Integrated Health OrganisationTekniker. Iñaki Goenaga 5Tekniker. Iñaki Goenaga 5Tekniker. Iñaki Goenaga 5Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BISTCatalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BISTCatalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BISTPropagroup S.P.a. - R&D DepartmentPropagroup S.P.a. - R&D DepartmentPropagroup S.P.a. - R&D DepartmentDepartment of Analytical Chemistry, Nutrition & Food Sciences, University of AlicanteDepartment of Analytical Chemistry, Nutrition & Food Sciences, University of AlicanteDepartment of Analytical Chemistry, Nutrition & Food Sciences, University of AlicanteAIMPLAS Technological Institute of PolymersAIMPLAS Technological Institute of PolymersMaterials Business Unit, Collaborative R&D Department, Ansys Inc3B’s Research Group, I3Bs—Research Institute On Biomaterials, Headquarters of the European Institute of Excellence On Tissue Engineering and Regenerative Medicine, Biodegradables and Biomimetics of University of MinhoBiodonostia, Infectious Diseases Area, Respiratory Infection and Antimicrobial Resistance Group, Microbiology Department, Osakidetza Basque Health Service, Donostialdea Integrated Health OrganisationAbstract One strategy to decrease the incidence of hospital-acquired infections is to avoid the survival of pathogens in the environment by the development of surfaces with antimicrobial activity. To study the antibacterial behaviour of active surfaces, different approaches have been developed of which ISO 22916 is the standard. To assess the performance of different testing methodologies to analyse the antibacterial activity of hydrophobic surface patterned plastics as part of a Horizon 2020 European research project. Four different testing methods were used to study the antibacterial activity of a patterned film, including the ISO 22916 standard, the immersion method, the touch-transfer inoculation method, and the swab inoculation method, this latter developed specifically for this project. The non-realistic test conditions of the ISO 22916 standard showed this method to be non-appropriate in the study of hydrophobic patterned surfaces. The immersion method also showed no differences between patterned films and smooth controls due to the lack of attachment of testing bacteria on both surfaces. The antibacterial activity of films could be demonstrated by the touch-transfer and the swab inoculation methods, that more precisely mimicked the way of high-touch surfaces contamination, and showed to be the best methodologies to test the antibacterial activity of patterned hydrophobic surfaces. A new ISO standard would be desirable as the reference method to study the antibacterial behaviour of patterned surfaces.https://doi.org/10.1038/s41598-021-85995-9 |
| spellingShingle | Ana Perez-Gavilan Joana Vieira de Castro Ainara Arana Santos Merino Aritz Retolaza Sofia A. Alves Achille Francone Nikolaos Kehagias Clivia M. Sotomayor-Torres Donato Cocina Renato Mortera Salvatore Crapanzano Carlos Javier Pelegrín María Carmen Garrigos Alfonso Jiménez Begoña Galindo Mari Carmen Araque Donna Dykeman Nuno M. Neves Jose Maria Marimón Antibacterial activity testing methods for hydrophobic patterned surfaces Scientific Reports |
| title | Antibacterial activity testing methods for hydrophobic patterned surfaces |
| title_full | Antibacterial activity testing methods for hydrophobic patterned surfaces |
| title_fullStr | Antibacterial activity testing methods for hydrophobic patterned surfaces |
| title_full_unstemmed | Antibacterial activity testing methods for hydrophobic patterned surfaces |
| title_short | Antibacterial activity testing methods for hydrophobic patterned surfaces |
| title_sort | antibacterial activity testing methods for hydrophobic patterned surfaces |
| url | https://doi.org/10.1038/s41598-021-85995-9 |
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