Intravital Fluorescence Videomicroscopy to Study Tumor Angiogenesis and Microcirculation
Angiogenesis and microcirculation play a central role in growth and metastasis of human neoplasms, and, thus, represent a major target for novel treatment strategies. Mechanistic analysis of processes involved in tumor vascularization, however, requires sophisticated in vivo experimental models and...
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| Format: | Article |
| Language: | English |
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Elsevier
2000-01-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1476558600800202 |
| _version_ | 1828941164832620544 |
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| author | Peter Vajkoczy Axel Ullrich Michael D. Menger |
| author_facet | Peter Vajkoczy Axel Ullrich Michael D. Menger |
| author_sort | Peter Vajkoczy |
| collection | DOAJ |
| description | Angiogenesis and microcirculation play a central role in growth and metastasis of human neoplasms, and, thus, represent a major target for novel treatment strategies. Mechanistic analysis of processes involved in tumor vascularization, however, requires sophisticated in vivo experimental models and techniques. Intravital microscopy allows direct assessment of tumor angiogenesis, microcirculation and overall perfusion. Its application to the study of tumor-induced neovascularization further provides information on molecular transport and delivery, intra- and extravascular cell-to-cell and cell-tomatrix interaction, as well as tumor oxygenation and metabolism. With the recent advances in the field of bioluminescence and fluorescent reporter genes, appropriate for in vivo imaging, the intravital fluorescent microscopic approach has to be considered a powerful tool to study microvascular, cellular and molecular mechanisms of tumor growth. |
| first_indexed | 2024-12-14T03:31:23Z |
| format | Article |
| id | doaj.art-9b4a76f863a44a56a7e348f8a89dfbfc |
| institution | Directory Open Access Journal |
| issn | 1476-5586 1522-8002 |
| language | English |
| last_indexed | 2024-12-14T03:31:23Z |
| publishDate | 2000-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj.art-9b4a76f863a44a56a7e348f8a89dfbfc2022-12-21T23:18:44ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022000-01-0121536110.1038/sj.neo.7900062Intravital Fluorescence Videomicroscopy to Study Tumor Angiogenesis and MicrocirculationPeter Vajkoczy0Axel Ullrich1Michael D. Menger2Department of Neurosurgery, Klinikum Mannheim, University of Heidelberg, Mannheim, GermanyDepartment of Molecular Biology, Max-Planck-Institut für Biochemie, Martinsried, GermanyInstitute for Clinical and Experimental Surgery, University of Saarland, Homburg/Saar, GermanyAngiogenesis and microcirculation play a central role in growth and metastasis of human neoplasms, and, thus, represent a major target for novel treatment strategies. Mechanistic analysis of processes involved in tumor vascularization, however, requires sophisticated in vivo experimental models and techniques. Intravital microscopy allows direct assessment of tumor angiogenesis, microcirculation and overall perfusion. Its application to the study of tumor-induced neovascularization further provides information on molecular transport and delivery, intra- and extravascular cell-to-cell and cell-tomatrix interaction, as well as tumor oxygenation and metabolism. With the recent advances in the field of bioluminescence and fluorescent reporter genes, appropriate for in vivo imaging, the intravital fluorescent microscopic approach has to be considered a powerful tool to study microvascular, cellular and molecular mechanisms of tumor growth.http://www.sciencedirect.com/science/article/pii/S1476558600800202cranial windowdorsal skinfold chamberin vivoperfusionvascularization |
| spellingShingle | Peter Vajkoczy Axel Ullrich Michael D. Menger Intravital Fluorescence Videomicroscopy to Study Tumor Angiogenesis and Microcirculation Neoplasia: An International Journal for Oncology Research cranial window dorsal skinfold chamber in vivo perfusion vascularization |
| title | Intravital Fluorescence Videomicroscopy to Study Tumor Angiogenesis and Microcirculation |
| title_full | Intravital Fluorescence Videomicroscopy to Study Tumor Angiogenesis and Microcirculation |
| title_fullStr | Intravital Fluorescence Videomicroscopy to Study Tumor Angiogenesis and Microcirculation |
| title_full_unstemmed | Intravital Fluorescence Videomicroscopy to Study Tumor Angiogenesis and Microcirculation |
| title_short | Intravital Fluorescence Videomicroscopy to Study Tumor Angiogenesis and Microcirculation |
| title_sort | intravital fluorescence videomicroscopy to study tumor angiogenesis and microcirculation |
| topic | cranial window dorsal skinfold chamber in vivo perfusion vascularization |
| url | http://www.sciencedirect.com/science/article/pii/S1476558600800202 |
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