Protective Effects of Perinatal Resveratrol on Bisphenol A Exposure-Induced Cardiovascular Alterations and Hepatic Steatosis in Adult Offspring Mice: A Histopathological Study
Perinatal bisphenol A (BPA) exposure promotes the risk of cardiovascular diseases in adulthood. Currently, there is a dire need to develop new therapeutic strategies and options to treat the adverse fetal programming consequences of this exposure. The present study explored the protective effects of...
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MDPI AG
2023-12-01
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| Online Access: | https://www.mdpi.com/2076-3417/13/24/13163 |
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| author | Srinivasa Rao Sirasanagandla Mohamed Al-Mushaiqri Fatma Al Ghafri Nadia Al-Abri Isehaq Al-Huseini |
| author_facet | Srinivasa Rao Sirasanagandla Mohamed Al-Mushaiqri Fatma Al Ghafri Nadia Al-Abri Isehaq Al-Huseini |
| author_sort | Srinivasa Rao Sirasanagandla |
| collection | DOAJ |
| description | Perinatal bisphenol A (BPA) exposure promotes the risk of cardiovascular diseases in adulthood. Currently, there is a dire need to develop new therapeutic strategies and options to treat the adverse fetal programming consequences of this exposure. The present study explored the protective effects of perinatal resveratrol (Rsv) administration on BPA exposure-induced adverse cardiovascular changes and hepatic steatosis in adult offspring mice. Pregnant apolipoprotein E-deficient mice were exposed to BPA in drinking water (1 μg/mL) or to both BPA (1 μg/mL) and Rsv (oral; 20 mg kg<sup>−1</sup> day<sup>−1</sup>) during the gestation and lactation periods. Tissues from the heart, liver, left kidney, and brachiocephalic artery from adult offspring (20 weeks old) were processed for staining with H and E, Masson’s trichrome, and Verhoeff–van Gieson and subsequent histology analysis. In both female and male mice who received Rsv supplementation, the following changes were observed in the brachiocephalic arterial wall: (a) a reduction in the BPA exposure-induced increased thickness ratio of the tunica intima to tunica media from 1.3 ± 1.1 µm to 0.5 ± 0.37 µm (<i>p</i> = 0.027) and 0.72 ± 0.58 µm to 0.29 ± 0.25 µm (<i>p</i> = 0.038), respectively, (b) a reduction in the number of elastic lamina breaks (<i>p</i> < 0.05), and (c) the prevention of the BPA exposure-induced progression of atherosclerotic lesions. Further, it also reduced the BPA exposure-induced increased left ventricular thickness by 135 µm and 131 µm in female and male offspring, respectively. The BPA exposure-induced hepatic steatosis score was also significantly reduced with Rsv treatment in female offspring mice (<i>p</i> = 0.02). Renal cortical cytoplasmic vacuolation was identified in both BPA and/or Rsv-treated groups. Our findings suggest that Rsv could be a potential protective candidate against perinatal BPA exposure-induced cardiovascular changes and hepatic steatosis. |
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| issn | 2076-3417 |
| language | English |
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| spelling | doaj.art-9b4d2bd881e742e4ade3fbd979e5b1792023-12-22T13:51:42ZengMDPI AGApplied Sciences2076-34172023-12-0113241316310.3390/app132413163Protective Effects of Perinatal Resveratrol on Bisphenol A Exposure-Induced Cardiovascular Alterations and Hepatic Steatosis in Adult Offspring Mice: A Histopathological StudySrinivasa Rao Sirasanagandla0Mohamed Al-Mushaiqri1Fatma Al Ghafri2Nadia Al-Abri3Isehaq Al-Huseini4Department of Human and Clinical Anatomy, College of Medicine and Health Sciences, Sultan Qaboos University, Al-Khoudh, Muscat 123, OmanDepartment of Human and Clinical Anatomy, College of Medicine and Health Sciences, Sultan Qaboos University, Al-Khoudh, Muscat 123, OmanDepartment of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Al-Khoudh, Muscat 123, OmanDepartment of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Al-Khoudh, Muscat 123, OmanDepartment of Physiology, College of Medicine and Health Sciences, Sultan Qaboos University, Al-Khoudh, Muscat 123, OmanPerinatal bisphenol A (BPA) exposure promotes the risk of cardiovascular diseases in adulthood. Currently, there is a dire need to develop new therapeutic strategies and options to treat the adverse fetal programming consequences of this exposure. The present study explored the protective effects of perinatal resveratrol (Rsv) administration on BPA exposure-induced adverse cardiovascular changes and hepatic steatosis in adult offspring mice. Pregnant apolipoprotein E-deficient mice were exposed to BPA in drinking water (1 μg/mL) or to both BPA (1 μg/mL) and Rsv (oral; 20 mg kg<sup>−1</sup> day<sup>−1</sup>) during the gestation and lactation periods. Tissues from the heart, liver, left kidney, and brachiocephalic artery from adult offspring (20 weeks old) were processed for staining with H and E, Masson’s trichrome, and Verhoeff–van Gieson and subsequent histology analysis. In both female and male mice who received Rsv supplementation, the following changes were observed in the brachiocephalic arterial wall: (a) a reduction in the BPA exposure-induced increased thickness ratio of the tunica intima to tunica media from 1.3 ± 1.1 µm to 0.5 ± 0.37 µm (<i>p</i> = 0.027) and 0.72 ± 0.58 µm to 0.29 ± 0.25 µm (<i>p</i> = 0.038), respectively, (b) a reduction in the number of elastic lamina breaks (<i>p</i> < 0.05), and (c) the prevention of the BPA exposure-induced progression of atherosclerotic lesions. Further, it also reduced the BPA exposure-induced increased left ventricular thickness by 135 µm and 131 µm in female and male offspring, respectively. The BPA exposure-induced hepatic steatosis score was also significantly reduced with Rsv treatment in female offspring mice (<i>p</i> = 0.02). Renal cortical cytoplasmic vacuolation was identified in both BPA and/or Rsv-treated groups. Our findings suggest that Rsv could be a potential protective candidate against perinatal BPA exposure-induced cardiovascular changes and hepatic steatosis.https://www.mdpi.com/2076-3417/13/24/13163bisphenol Aresveratrolcardiovascular diseasehearthepatic steatosis |
| spellingShingle | Srinivasa Rao Sirasanagandla Mohamed Al-Mushaiqri Fatma Al Ghafri Nadia Al-Abri Isehaq Al-Huseini Protective Effects of Perinatal Resveratrol on Bisphenol A Exposure-Induced Cardiovascular Alterations and Hepatic Steatosis in Adult Offspring Mice: A Histopathological Study Applied Sciences bisphenol A resveratrol cardiovascular disease heart hepatic steatosis |
| title | Protective Effects of Perinatal Resveratrol on Bisphenol A Exposure-Induced Cardiovascular Alterations and Hepatic Steatosis in Adult Offspring Mice: A Histopathological Study |
| title_full | Protective Effects of Perinatal Resveratrol on Bisphenol A Exposure-Induced Cardiovascular Alterations and Hepatic Steatosis in Adult Offspring Mice: A Histopathological Study |
| title_fullStr | Protective Effects of Perinatal Resveratrol on Bisphenol A Exposure-Induced Cardiovascular Alterations and Hepatic Steatosis in Adult Offspring Mice: A Histopathological Study |
| title_full_unstemmed | Protective Effects of Perinatal Resveratrol on Bisphenol A Exposure-Induced Cardiovascular Alterations and Hepatic Steatosis in Adult Offspring Mice: A Histopathological Study |
| title_short | Protective Effects of Perinatal Resveratrol on Bisphenol A Exposure-Induced Cardiovascular Alterations and Hepatic Steatosis in Adult Offspring Mice: A Histopathological Study |
| title_sort | protective effects of perinatal resveratrol on bisphenol a exposure induced cardiovascular alterations and hepatic steatosis in adult offspring mice a histopathological study |
| topic | bisphenol A resveratrol cardiovascular disease heart hepatic steatosis |
| url | https://www.mdpi.com/2076-3417/13/24/13163 |
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