Quantitative Differences in Rumen Epithelium Proteins in Lambs Fed Wheat, Perennial Wheat, or Perennial Wheat plus Lucerne
The value of crops such as perennial wheat (PW) for grain and grazing compared to conventional wheat (W), or the addition of lucerne to PW (PWL) is still being determined. This research sought to determine if these diets were associated with changes in the membranebound proteins that transport nutri...
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MDPI AG
2023-09-01
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| Series: | Proteomes |
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| Online Access: | https://www.mdpi.com/2227-7382/11/3/27 |
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| author | Jude Jessie Bond Gordon Refshauge Matthew T. Newell Benjamin W. B. Holman David Wheeler Serey Woodgate Karthik S. Kamath Richard C. Hayes |
| author_facet | Jude Jessie Bond Gordon Refshauge Matthew T. Newell Benjamin W. B. Holman David Wheeler Serey Woodgate Karthik S. Kamath Richard C. Hayes |
| author_sort | Jude Jessie Bond |
| collection | DOAJ |
| description | The value of crops such as perennial wheat (PW) for grain and grazing compared to conventional wheat (W), or the addition of lucerne to PW (PWL) is still being determined. This research sought to determine if these diets were associated with changes in the membranebound proteins that transport nutrients in the rumen epithelium (RE). Crossbred ewes (Poll Dorset × Merino) were fed W, PW, or PWL (50:50) fresh-cut forage <i>ad libitum</i> for 4 weeks. Average daily gain (ADG; <i>p</i> < 0.001) was highest in the W-fed lambs compared to the PW and PWL. Metabolisable energy intake (MEI) was higher in lambs fed W (<i>p</i> < 0.001) compared to PW and PWL. In pairwise comparisons of the PW and PWL diet group we found protein abundance was significantly (<i>p</i> < 0.05, FDR < 0.05, Benjamini <i>p</i> < 0.05) different in fatty acid metabolism, oxidative phosphorylation, and biosynthesis of cofactors pathways. There were not any differences in protein abundance related to nutrient transport or energy metabolism in the RE between W- vs. PW- and W- vs. PWL-fed lambs. However, in the PW- vs. PWL-fed lambs, there was a difference in the level of proteins regulating the metabolism of fatty acids and energy production in the mitochondria of the rumen epithelium. |
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| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-03-10T22:08:35Z |
| publishDate | 2023-09-01 |
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| series | Proteomes |
| spelling | doaj.art-9b4e211377774e53a92d93926c7377232023-11-19T12:43:21ZengMDPI AGProteomes2227-73822023-09-011132710.3390/proteomes11030027Quantitative Differences in Rumen Epithelium Proteins in Lambs Fed Wheat, Perennial Wheat, or Perennial Wheat plus LucerneJude Jessie Bond0Gordon Refshauge1Matthew T. Newell2Benjamin W. B. Holman3David Wheeler4Serey Woodgate5Karthik S. Kamath6Richard C. Hayes7NSW Department of Primary Industries, Extensive Livestock Industry Centre, Trevenna Rd, University of New England, Armidale, NSW 2351, AustraliaCowra Agricultural Research and Advisory Station, NSW Department of Primary Industries, Cowra, NSW 2794, AustraliaCowra Agricultural Research and Advisory Station, NSW Department of Primary Industries, Cowra, NSW 2794, AustraliaWagga Wagga Agricultural Institute, NSW Department of Primary Industries, Wagga Wagga, NSW 2650, AustraliaNSW Department of Primary Industries, Orange Agricultural Institute, Orange, NSW 2800, AustraliaNSW Department of Primary Industries, Extensive Livestock Industry Centre, Trevenna Rd, University of New England, Armidale, NSW 2351, AustraliaAustralian Proteome Analysis Facility (APAF), Macquarie University, North Ryde, NSW 2109, AustraliaCowra Agricultural Research and Advisory Station, NSW Department of Primary Industries, Cowra, NSW 2794, AustraliaThe value of crops such as perennial wheat (PW) for grain and grazing compared to conventional wheat (W), or the addition of lucerne to PW (PWL) is still being determined. This research sought to determine if these diets were associated with changes in the membranebound proteins that transport nutrients in the rumen epithelium (RE). Crossbred ewes (Poll Dorset × Merino) were fed W, PW, or PWL (50:50) fresh-cut forage <i>ad libitum</i> for 4 weeks. Average daily gain (ADG; <i>p</i> < 0.001) was highest in the W-fed lambs compared to the PW and PWL. Metabolisable energy intake (MEI) was higher in lambs fed W (<i>p</i> < 0.001) compared to PW and PWL. In pairwise comparisons of the PW and PWL diet group we found protein abundance was significantly (<i>p</i> < 0.05, FDR < 0.05, Benjamini <i>p</i> < 0.05) different in fatty acid metabolism, oxidative phosphorylation, and biosynthesis of cofactors pathways. There were not any differences in protein abundance related to nutrient transport or energy metabolism in the RE between W- vs. PW- and W- vs. PWL-fed lambs. However, in the PW- vs. PWL-fed lambs, there was a difference in the level of proteins regulating the metabolism of fatty acids and energy production in the mitochondria of the rumen epithelium.https://www.mdpi.com/2227-7382/11/3/27nutrienttransportmembranerumenepithelium |
| spellingShingle | Jude Jessie Bond Gordon Refshauge Matthew T. Newell Benjamin W. B. Holman David Wheeler Serey Woodgate Karthik S. Kamath Richard C. Hayes Quantitative Differences in Rumen Epithelium Proteins in Lambs Fed Wheat, Perennial Wheat, or Perennial Wheat plus Lucerne Proteomes nutrient transport membrane rumen epithelium |
| title | Quantitative Differences in Rumen Epithelium Proteins in Lambs Fed Wheat, Perennial Wheat, or Perennial Wheat plus Lucerne |
| title_full | Quantitative Differences in Rumen Epithelium Proteins in Lambs Fed Wheat, Perennial Wheat, or Perennial Wheat plus Lucerne |
| title_fullStr | Quantitative Differences in Rumen Epithelium Proteins in Lambs Fed Wheat, Perennial Wheat, or Perennial Wheat plus Lucerne |
| title_full_unstemmed | Quantitative Differences in Rumen Epithelium Proteins in Lambs Fed Wheat, Perennial Wheat, or Perennial Wheat plus Lucerne |
| title_short | Quantitative Differences in Rumen Epithelium Proteins in Lambs Fed Wheat, Perennial Wheat, or Perennial Wheat plus Lucerne |
| title_sort | quantitative differences in rumen epithelium proteins in lambs fed wheat perennial wheat or perennial wheat plus lucerne |
| topic | nutrient transport membrane rumen epithelium |
| url | https://www.mdpi.com/2227-7382/11/3/27 |
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