Diagnostic proficiency test using digital cytopathology and comparative assessment of whole slide images of cytologic samples for quality assurance program in Korea
Background The Korean Society for Cytopathology introduced a digital proficiency test (PT) in 2021. However, many doubtful opinions remain on whether digitally scanned images can satisfactorily present subtle differences in the nuclear features and chromatin patterns of cytological samples. Methods...
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| Format: | Article |
| Language: | English |
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Korean Society of Pathologists & the Korean Society for Cytopathology
2023-09-01
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| Series: | Journal of Pathology and Translational Medicine |
| Subjects: | |
| Online Access: | http://jpatholtm.org/upload/pdf/jptm-2023-07-17.pdf |
| _version_ | 1797679309354172416 |
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| author | Yosep Chong Soon Auck Hong Hoon Kyu Oh Soo Jin Jung Bo-Sung Kim Ji Yun Jeong Ho-Chang Lee Gyungyub Gong |
| author_facet | Yosep Chong Soon Auck Hong Hoon Kyu Oh Soo Jin Jung Bo-Sung Kim Ji Yun Jeong Ho-Chang Lee Gyungyub Gong |
| author_sort | Yosep Chong |
| collection | DOAJ |
| description | Background The Korean Society for Cytopathology introduced a digital proficiency test (PT) in 2021. However, many doubtful opinions remain on whether digitally scanned images can satisfactorily present subtle differences in the nuclear features and chromatin patterns of cytological samples. Methods We prepared 30 whole-slide images (WSIs) from the conventional PT archive by a selection process for digital PT. Digital and conventional PT were performed in parallel for volunteer institutes, and the results were compared using feedback. To assess the quality of cytological assessment WSIs, 12 slides were collected and scanned using five different scanners, with four cytopathologists evaluating image quality through a questionnaire. Results Among the 215 institutes, 108 and 107 participated in glass and digital PT, respectively. No significant difference was noted in category C (major discordance), although the number of discordant cases was slightly higher in the digital PT group. Leica, 3DHistech Pannoramic 250 Flash, and Hamamatsu NanoZoomer 360 systems showed comparable results in terms of image quality, feature presentation, and error rates for most cytological samples. Overall satisfaction was observed with the general convenience and image quality of digital PT. Conclusions As three-dimensional clusters are common and nuclear/chromatin features are critical for cytological interpretation, careful selection of scanners and optimal conditions are mandatory for the successful establishment of digital quality assurance programs in cytology. |
| first_indexed | 2024-03-11T23:12:39Z |
| format | Article |
| id | doaj.art-9b4f4f9ba58a48f5b5e0265dcff45974 |
| institution | Directory Open Access Journal |
| issn | 2383-7837 2383-7845 |
| language | English |
| last_indexed | 2024-03-11T23:12:39Z |
| publishDate | 2023-09-01 |
| publisher | Korean Society of Pathologists & the Korean Society for Cytopathology |
| record_format | Article |
| series | Journal of Pathology and Translational Medicine |
| spelling | doaj.art-9b4f4f9ba58a48f5b5e0265dcff459742023-09-21T05:57:32ZengKorean Society of Pathologists & the Korean Society for CytopathologyJournal of Pathology and Translational Medicine2383-78372383-78452023-09-0157525126410.4132/jptm.2023.07.1717080Diagnostic proficiency test using digital cytopathology and comparative assessment of whole slide images of cytologic samples for quality assurance program in KoreaYosep Chong0Soon Auck Hong1Hoon Kyu Oh2Soo Jin Jung3Bo-Sung Kim4Ji Yun Jeong5Ho-Chang Lee6Gyungyub Gong7 Department of Hospital Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea Department of Pathology, Chung-Ang University College of Medicine, Seoul, Korea Department of Pathology, Daegu Catholic University School of Medicine, Daegu, Korea Department of Pathology, Inje University Busan Paik Hospital, Busan, Korea Department of Pathology, Green Cross Laboratories, Yongin, Korea Department of Pathology, Kyungpook National University Chilgok Hospital, School of Medicine, Kyungpook National University, Daegu, Korea Department of Pathology, Chungbuk National University College of Medicine, Cheongju, Korea Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, KoreaBackground The Korean Society for Cytopathology introduced a digital proficiency test (PT) in 2021. However, many doubtful opinions remain on whether digitally scanned images can satisfactorily present subtle differences in the nuclear features and chromatin patterns of cytological samples. Methods We prepared 30 whole-slide images (WSIs) from the conventional PT archive by a selection process for digital PT. Digital and conventional PT were performed in parallel for volunteer institutes, and the results were compared using feedback. To assess the quality of cytological assessment WSIs, 12 slides were collected and scanned using five different scanners, with four cytopathologists evaluating image quality through a questionnaire. Results Among the 215 institutes, 108 and 107 participated in glass and digital PT, respectively. No significant difference was noted in category C (major discordance), although the number of discordant cases was slightly higher in the digital PT group. Leica, 3DHistech Pannoramic 250 Flash, and Hamamatsu NanoZoomer 360 systems showed comparable results in terms of image quality, feature presentation, and error rates for most cytological samples. Overall satisfaction was observed with the general convenience and image quality of digital PT. Conclusions As three-dimensional clusters are common and nuclear/chromatin features are critical for cytological interpretation, careful selection of scanners and optimal conditions are mandatory for the successful establishment of digital quality assurance programs in cytology.http://jpatholtm.org/upload/pdf/jptm-2023-07-17.pdfcytologyquality assurancedigital pathologywhole-slide imagewhole-slide scanner |
| spellingShingle | Yosep Chong Soon Auck Hong Hoon Kyu Oh Soo Jin Jung Bo-Sung Kim Ji Yun Jeong Ho-Chang Lee Gyungyub Gong Diagnostic proficiency test using digital cytopathology and comparative assessment of whole slide images of cytologic samples for quality assurance program in Korea Journal of Pathology and Translational Medicine cytology quality assurance digital pathology whole-slide image whole-slide scanner |
| title | Diagnostic proficiency test using digital cytopathology and comparative assessment of whole slide images of cytologic samples for quality assurance program in Korea |
| title_full | Diagnostic proficiency test using digital cytopathology and comparative assessment of whole slide images of cytologic samples for quality assurance program in Korea |
| title_fullStr | Diagnostic proficiency test using digital cytopathology and comparative assessment of whole slide images of cytologic samples for quality assurance program in Korea |
| title_full_unstemmed | Diagnostic proficiency test using digital cytopathology and comparative assessment of whole slide images of cytologic samples for quality assurance program in Korea |
| title_short | Diagnostic proficiency test using digital cytopathology and comparative assessment of whole slide images of cytologic samples for quality assurance program in Korea |
| title_sort | diagnostic proficiency test using digital cytopathology and comparative assessment of whole slide images of cytologic samples for quality assurance program in korea |
| topic | cytology quality assurance digital pathology whole-slide image whole-slide scanner |
| url | http://jpatholtm.org/upload/pdf/jptm-2023-07-17.pdf |
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