Radiation-Induced Dual Oxidase Upregulation in Rat Heart Tissues: Protective Effect of Melatonin
<i>Background</i>: Radiation-induced heart injury can lead to increased risk of heart failure, attack, and ischemia. Some studies proposed IL-4 and IL-13 as two important cytokines that are involved in late effects of ionizing radiation. On the other hand, these cytokines may, through up...
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| Format: | Article |
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MDPI AG
2019-06-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1010-660X/55/7/317 |
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| author | Bagher Farhood Akbar Aliasgharzadeh Peyman Amini Hana Saffar Elahe Motevaseli Saeed Rezapoor Farzad Nouruzi Dheyauldeen Shabeeb Ahmed Eleojo Musa Ghorbangol Ashabi Mehran Mohseni Habiballah Moradi Masoud Najafi |
| author_facet | Bagher Farhood Akbar Aliasgharzadeh Peyman Amini Hana Saffar Elahe Motevaseli Saeed Rezapoor Farzad Nouruzi Dheyauldeen Shabeeb Ahmed Eleojo Musa Ghorbangol Ashabi Mehran Mohseni Habiballah Moradi Masoud Najafi |
| author_sort | Bagher Farhood |
| collection | DOAJ |
| description | <i>Background</i>: Radiation-induced heart injury can lead to increased risk of heart failure, attack, and ischemia. Some studies proposed IL-4 and IL-13 as two important cytokines that are involved in late effects of ionizing radiation. On the other hand, these cytokines may, through upregulation of <i>Duox1</i> and <i>Duox2</i>, induce chronic oxidative stress, inflammation, and fibrosis. In this study, we evaluated the upregulation of <i>Duox1</i> and <i>Duox2</i> pathways in hearts following chest irradiation in rats and then detected possible attenuation of them by melatonin. <i>Materials and Methods</i>: Twenty male Wistar rats were divided into four groups: (1) control; (2) melatonin treated (100 mg/kg); (3) radiation (15 Gy gamma rays); (4) melatonin treated before irradiation. All rats were sacrificed after 10 weeks and their heart tissues collected for real-time PCR (RT-PCR), ELISA detection of IL-4 and IL-13, as well as histopathological evaluation of macrophages and lymphocytes infiltration. <i>Results</i>: Results showed an upregulation of IL-4, <i>IL4ra1</i>, <i>Duox1</i>, and <i>Duox2</i>. The biggest changes were for <i>IL4ra1</i> and <i>Duox1</i>. Treatment with melatonin before irradiation could attenuate the upregulation of all genes. Melatonin also caused a reduction in IL-4 as well as reverse infiltration of inflammatory cells. <i>Conclusion</i>: <i>Duox1</i> and <i>Duox2</i> may be involved in the late effects of radiation-induced heart injury. Also, via attenuation of these genes, melatonin can offer protection against the toxic effects of radiation on the heart. |
| first_indexed | 2024-03-12T06:03:01Z |
| format | Article |
| id | doaj.art-9b505a84a21a4a62a1efdecce6a1f876 |
| institution | Directory Open Access Journal |
| issn | 1010-660X |
| language | English |
| last_indexed | 2024-03-12T06:03:01Z |
| publishDate | 2019-06-01 |
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| record_format | Article |
| series | Medicina |
| spelling | doaj.art-9b505a84a21a4a62a1efdecce6a1f8762023-09-03T03:58:09ZengMDPI AGMedicina1010-660X2019-06-0155731710.3390/medicina55070317medicina55070317Radiation-Induced Dual Oxidase Upregulation in Rat Heart Tissues: Protective Effect of MelatoninBagher Farhood0Akbar Aliasgharzadeh1Peyman Amini2Hana Saffar3Elahe Motevaseli4Saeed Rezapoor5Farzad Nouruzi6Dheyauldeen Shabeeb7Ahmed Eleojo Musa8Ghorbangol Ashabi9Mehran Mohseni10Habiballah Moradi11Masoud Najafi12Departments of Medical Physics and Radiology, Faculty of Paramedical Sciences, Kashan University of Medical Sciences, 8715988141 Kashan, IranDepartments of Medical Physics and Radiology, Faculty of Paramedical Sciences, Kashan University of Medical Sciences, 8715988141 Kashan, IranDepartment of Radiology, Faculty of Paramedical, Tehran University of Medical Sciences, 1416753955 Tehran, IranClinical and Anatomical Pathologist at Tehran University of Medical Science, Imam Khomeini Hospital Complex, 1419733141 Tehran, IranDepartment of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, 1416753955 Tehran, IranDepartment of Radiology, Faculty of Paramedical, Tehran University of Medical Sciences, 1416753955 Tehran, IranDepartment of Medical Radiation Engineering, Science and Research Branch, Islamic Azad University, 1477893855 Tehran, IranDepartment of Physiology, College of Medicine, University of Misan, 62010 Misan, IraqDepartment of Medical Physics, Tehran University of Medical Sciences (International Campus), 1416753955 Tehran, IranDepartment of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, 1416753955 Tehran, IranDepartments of Medical Physics and Radiology, Faculty of Paramedical Sciences, Kashan University of Medical Sciences, 8715988141 Kashan, IranDepartments of Medical Physics and Radiology, Faculty of Paramedical Sciences, Kashan University of Medical Sciences, 8715988141 Kashan, IranRadiology and Nuclear Medicine Department, School of Paramedical Sciences, Kermanshah University of Medical Sciences, 6715847141 Kermanshah, Iran<i>Background</i>: Radiation-induced heart injury can lead to increased risk of heart failure, attack, and ischemia. Some studies proposed IL-4 and IL-13 as two important cytokines that are involved in late effects of ionizing radiation. On the other hand, these cytokines may, through upregulation of <i>Duox1</i> and <i>Duox2</i>, induce chronic oxidative stress, inflammation, and fibrosis. In this study, we evaluated the upregulation of <i>Duox1</i> and <i>Duox2</i> pathways in hearts following chest irradiation in rats and then detected possible attenuation of them by melatonin. <i>Materials and Methods</i>: Twenty male Wistar rats were divided into four groups: (1) control; (2) melatonin treated (100 mg/kg); (3) radiation (15 Gy gamma rays); (4) melatonin treated before irradiation. All rats were sacrificed after 10 weeks and their heart tissues collected for real-time PCR (RT-PCR), ELISA detection of IL-4 and IL-13, as well as histopathological evaluation of macrophages and lymphocytes infiltration. <i>Results</i>: Results showed an upregulation of IL-4, <i>IL4ra1</i>, <i>Duox1</i>, and <i>Duox2</i>. The biggest changes were for <i>IL4ra1</i> and <i>Duox1</i>. Treatment with melatonin before irradiation could attenuate the upregulation of all genes. Melatonin also caused a reduction in IL-4 as well as reverse infiltration of inflammatory cells. <i>Conclusion</i>: <i>Duox1</i> and <i>Duox2</i> may be involved in the late effects of radiation-induced heart injury. Also, via attenuation of these genes, melatonin can offer protection against the toxic effects of radiation on the heart.https://www.mdpi.com/1010-660X/55/7/317radiationmelatoninIL-4<i>Duox1</i><i>Duox2</i>heart |
| spellingShingle | Bagher Farhood Akbar Aliasgharzadeh Peyman Amini Hana Saffar Elahe Motevaseli Saeed Rezapoor Farzad Nouruzi Dheyauldeen Shabeeb Ahmed Eleojo Musa Ghorbangol Ashabi Mehran Mohseni Habiballah Moradi Masoud Najafi Radiation-Induced Dual Oxidase Upregulation in Rat Heart Tissues: Protective Effect of Melatonin Medicina radiation melatonin IL-4 <i>Duox1</i> <i>Duox2</i> heart |
| title | Radiation-Induced Dual Oxidase Upregulation in Rat Heart Tissues: Protective Effect of Melatonin |
| title_full | Radiation-Induced Dual Oxidase Upregulation in Rat Heart Tissues: Protective Effect of Melatonin |
| title_fullStr | Radiation-Induced Dual Oxidase Upregulation in Rat Heart Tissues: Protective Effect of Melatonin |
| title_full_unstemmed | Radiation-Induced Dual Oxidase Upregulation in Rat Heart Tissues: Protective Effect of Melatonin |
| title_short | Radiation-Induced Dual Oxidase Upregulation in Rat Heart Tissues: Protective Effect of Melatonin |
| title_sort | radiation induced dual oxidase upregulation in rat heart tissues protective effect of melatonin |
| topic | radiation melatonin IL-4 <i>Duox1</i> <i>Duox2</i> heart |
| url | https://www.mdpi.com/1010-660X/55/7/317 |
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