Global actions of nicotine on the striatal microcircuit
The question to solve in the present work is: what is the predominant action induced by the activation of cholinergic-nicotinic receptors (nAChrs) in the striatal network given that nAChrs are expressed by several elements of the circuit: cortical terminals, dopamine terminals, and various striatal...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2013-11-01
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| Series: | Frontiers in Systems Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnsys.2013.00078/full |
| _version_ | 1818852066911584256 |
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| author | Victor E Plata Mariana eDuhne Jesús Esteban Pérez-Ortega Ricardo eHernandez-Martinez Pavel Ernesto Rueda Orozco Elvira eGalarraga Rene eDrucker-Colin Jose eBargas |
| author_facet | Victor E Plata Mariana eDuhne Jesús Esteban Pérez-Ortega Ricardo eHernandez-Martinez Pavel Ernesto Rueda Orozco Elvira eGalarraga Rene eDrucker-Colin Jose eBargas |
| author_sort | Victor E Plata |
| collection | DOAJ |
| description | The question to solve in the present work is: what is the predominant action induced by the activation of cholinergic-nicotinic receptors (nAChrs) in the striatal network given that nAChrs are expressed by several elements of the circuit: cortical terminals, dopamine terminals, and various striatal GABAergic interneurons. To answer this question some type of multicellular recording has to be used without losing single cell resolution. Here, we used calcium imaging and nicotine. It is known that in the presence of low micromolar N-Methyl-D-aspartate (NMDA), the striatal microcircuit exhibits neuronal activity consisting in the spontaneous synchronization of different neuron pools that interchange their activity following determined sequences. The striatal circuit also exhibits profuse spontaneous activity in pathological states (without NMDA) such as dopamine depletion. However, in this case, most pathological activity is mostly generated by the same neuron pool. Here, we show that both types of activity are inhibited during the application of nicotine. Nicotine actions were blocked by mecamylamine, a non specific antagonist of nAChrs. Interestingly, inhibitory actions of nicotine were also blocked by the GABAA-receptor antagonist bicuculline, in which case, the actions of nicotine on the circuit became excitatory and facilitated neuronal synchronization. We conclude that the predominant action of nicotine in the striatal microcircuit is indirect, via the activation of networks of inhibitory interneurons. This action inhibits striatal pathological activity in early Parkinsonian animals almost as potently as L-DOPA. |
| first_indexed | 2024-12-19T07:15:01Z |
| format | Article |
| id | doaj.art-9b51c571a9994f90b294052efe744d2a |
| institution | Directory Open Access Journal |
| issn | 1662-5137 |
| language | English |
| last_indexed | 2024-12-19T07:15:01Z |
| publishDate | 2013-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Systems Neuroscience |
| spelling | doaj.art-9b51c571a9994f90b294052efe744d2a2022-12-21T20:31:06ZengFrontiers Media S.A.Frontiers in Systems Neuroscience1662-51372013-11-01710.3389/fnsys.2013.0007864926Global actions of nicotine on the striatal microcircuitVictor E Plata0Mariana eDuhne1Jesús Esteban Pérez-Ortega2Ricardo eHernandez-Martinez3Pavel Ernesto Rueda Orozco4Elvira eGalarraga5Rene eDrucker-Colin6Jose eBargas7Universidad Nacional Autónoma de MéxicoUniversidad Nacional Autónoma de MéxicoUniversidad Nacional Autónoma de MéxicoUniversidad Nacional Autónoma de MéxicoUniversidad Nacional Autónoma de MéxicoUniversidad Nacional Autónoma de MéxicoUniversidad Nacional Autónoma de México (University, Governmental)Universidad Nacional Autónoma de MéxicoThe question to solve in the present work is: what is the predominant action induced by the activation of cholinergic-nicotinic receptors (nAChrs) in the striatal network given that nAChrs are expressed by several elements of the circuit: cortical terminals, dopamine terminals, and various striatal GABAergic interneurons. To answer this question some type of multicellular recording has to be used without losing single cell resolution. Here, we used calcium imaging and nicotine. It is known that in the presence of low micromolar N-Methyl-D-aspartate (NMDA), the striatal microcircuit exhibits neuronal activity consisting in the spontaneous synchronization of different neuron pools that interchange their activity following determined sequences. The striatal circuit also exhibits profuse spontaneous activity in pathological states (without NMDA) such as dopamine depletion. However, in this case, most pathological activity is mostly generated by the same neuron pool. Here, we show that both types of activity are inhibited during the application of nicotine. Nicotine actions were blocked by mecamylamine, a non specific antagonist of nAChrs. Interestingly, inhibitory actions of nicotine were also blocked by the GABAA-receptor antagonist bicuculline, in which case, the actions of nicotine on the circuit became excitatory and facilitated neuronal synchronization. We conclude that the predominant action of nicotine in the striatal microcircuit is indirect, via the activation of networks of inhibitory interneurons. This action inhibits striatal pathological activity in early Parkinsonian animals almost as potently as L-DOPA.http://journal.frontiersin.org/Journal/10.3389/fnsys.2013.00078/fullBasal GangliaNicotinenicotinic receptorsGABAergic interneuronsparkinson´s diseasestriatal microcircuit |
| spellingShingle | Victor E Plata Mariana eDuhne Jesús Esteban Pérez-Ortega Ricardo eHernandez-Martinez Pavel Ernesto Rueda Orozco Elvira eGalarraga Rene eDrucker-Colin Jose eBargas Global actions of nicotine on the striatal microcircuit Frontiers in Systems Neuroscience Basal Ganglia Nicotine nicotinic receptors GABAergic interneurons parkinson´s disease striatal microcircuit |
| title | Global actions of nicotine on the striatal microcircuit |
| title_full | Global actions of nicotine on the striatal microcircuit |
| title_fullStr | Global actions of nicotine on the striatal microcircuit |
| title_full_unstemmed | Global actions of nicotine on the striatal microcircuit |
| title_short | Global actions of nicotine on the striatal microcircuit |
| title_sort | global actions of nicotine on the striatal microcircuit |
| topic | Basal Ganglia Nicotine nicotinic receptors GABAergic interneurons parkinson´s disease striatal microcircuit |
| url | http://journal.frontiersin.org/Journal/10.3389/fnsys.2013.00078/full |
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