The Effect of Zinc, Copper, and Silver Ions on Oat (<i>Avena sativa</i> L.) Androgenesis
Oat (<i>Avena sativa</i> L.) cultivars ‘Bingo’ and ‘Chwat’ were used to compare the embryogenesis competence of another culture. Despite the embryo-like structures obtained from both tested cultivars, only ‘Chwat’ produced green plantlets, which confirmed the cultivar dependency. ‘Chwat’...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/2/248 |
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| author | Marzena Warchoł Katarzyna Juzoń Kinga Dziurka Ilona Czyczyło-Mysza Kamila Kapłoniak Izabela Marcińska Edyta Skrzypek |
| author_facet | Marzena Warchoł Katarzyna Juzoń Kinga Dziurka Ilona Czyczyło-Mysza Kamila Kapłoniak Izabela Marcińska Edyta Skrzypek |
| author_sort | Marzena Warchoł |
| collection | DOAJ |
| description | Oat (<i>Avena sativa</i> L.) cultivars ‘Bingo’ and ‘Chwat’ were used to compare the embryogenesis competence of another culture. Despite the embryo-like structures obtained from both tested cultivars, only ‘Chwat’ produced green plantlets, which confirmed the cultivar dependency. ‘Chwat’ produced the highest number of embryo-like structures and green plantlets (0.7/100 anthers and 0.1/100 anthers, respectively). The embryo-like structure formation also depended on cold pretreatment combined with Cu<sup>2+</sup>, Zn<sup>2+</sup>, or Ag<sup>+</sup> ion supplementation, which was applied during the tiller pretreatment or added to the induction media. The highest number of embryo-like structures (2.1/100 anthers) were observed on anthers derived from the tillers kept in a 50% Hoagland medium with the addition of 10 µM of CuSO<sub>4</sub>. In turn, the induction media supplemented with the ions Cu<sup>2+</sup>, Zn<sup>2+</sup>, or Ag<sup>+</sup> increased neither the number of embryo-like structures nor the green plantlet production compared to the control conditions. However, such ion applications turned out to be most effective when the induction medium was enriched with 25 µM of AgNO<sub>3</sub> and left to obtain the highest number of embryo-like structures and green plantlets (0.8/100 anthers and 0.2/100 anthers, respectively). Therefore, more attention should be paid to the possibilities of adjusting the media nutrient composition, as this may be the only way to significantly increase the efficiency of this method. |
| first_indexed | 2024-03-09T03:27:34Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T03:27:34Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-9b5367a44d9c4011b2519ffaa8ccf40d2023-12-03T15:00:08ZengMDPI AGPlants2223-77472021-01-0110224810.3390/plants10020248The Effect of Zinc, Copper, and Silver Ions on Oat (<i>Avena sativa</i> L.) AndrogenesisMarzena Warchoł0Katarzyna Juzoń1Kinga Dziurka2Ilona Czyczyło-Mysza3Kamila Kapłoniak4Izabela Marcińska5Edyta Skrzypek6Department of Biotechnology, The <i>Franciszek Górski</i> Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, PolandDepartment of Biotechnology, The <i>Franciszek Górski</i> Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, PolandDepartment of Biotechnology, The <i>Franciszek Górski</i> Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, PolandDepartment of Biotechnology, The <i>Franciszek Górski</i> Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, PolandDepartment of Biotechnology, The <i>Franciszek Górski</i> Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, PolandDepartment of Biotechnology, The <i>Franciszek Górski</i> Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, PolandDepartment of Biotechnology, The <i>Franciszek Górski</i> Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, PolandOat (<i>Avena sativa</i> L.) cultivars ‘Bingo’ and ‘Chwat’ were used to compare the embryogenesis competence of another culture. Despite the embryo-like structures obtained from both tested cultivars, only ‘Chwat’ produced green plantlets, which confirmed the cultivar dependency. ‘Chwat’ produced the highest number of embryo-like structures and green plantlets (0.7/100 anthers and 0.1/100 anthers, respectively). The embryo-like structure formation also depended on cold pretreatment combined with Cu<sup>2+</sup>, Zn<sup>2+</sup>, or Ag<sup>+</sup> ion supplementation, which was applied during the tiller pretreatment or added to the induction media. The highest number of embryo-like structures (2.1/100 anthers) were observed on anthers derived from the tillers kept in a 50% Hoagland medium with the addition of 10 µM of CuSO<sub>4</sub>. In turn, the induction media supplemented with the ions Cu<sup>2+</sup>, Zn<sup>2+</sup>, or Ag<sup>+</sup> increased neither the number of embryo-like structures nor the green plantlet production compared to the control conditions. However, such ion applications turned out to be most effective when the induction medium was enriched with 25 µM of AgNO<sub>3</sub> and left to obtain the highest number of embryo-like structures and green plantlets (0.8/100 anthers and 0.2/100 anthers, respectively). Therefore, more attention should be paid to the possibilities of adjusting the media nutrient composition, as this may be the only way to significantly increase the efficiency of this method.https://www.mdpi.com/2223-7747/10/2/248androgenesisgreen plantletspretreatmentZn<sup>2+</sup>, Cu<sup>2+</sup>, and Ag<sup>+</sup> ions |
| spellingShingle | Marzena Warchoł Katarzyna Juzoń Kinga Dziurka Ilona Czyczyło-Mysza Kamila Kapłoniak Izabela Marcińska Edyta Skrzypek The Effect of Zinc, Copper, and Silver Ions on Oat (<i>Avena sativa</i> L.) Androgenesis Plants androgenesis green plantlets pretreatment Zn<sup>2+</sup>, Cu<sup>2+</sup>, and Ag<sup>+</sup> ions |
| title | The Effect of Zinc, Copper, and Silver Ions on Oat (<i>Avena sativa</i> L.) Androgenesis |
| title_full | The Effect of Zinc, Copper, and Silver Ions on Oat (<i>Avena sativa</i> L.) Androgenesis |
| title_fullStr | The Effect of Zinc, Copper, and Silver Ions on Oat (<i>Avena sativa</i> L.) Androgenesis |
| title_full_unstemmed | The Effect of Zinc, Copper, and Silver Ions on Oat (<i>Avena sativa</i> L.) Androgenesis |
| title_short | The Effect of Zinc, Copper, and Silver Ions on Oat (<i>Avena sativa</i> L.) Androgenesis |
| title_sort | effect of zinc copper and silver ions on oat i avena sativa i l androgenesis |
| topic | androgenesis green plantlets pretreatment Zn<sup>2+</sup>, Cu<sup>2+</sup>, and Ag<sup>+</sup> ions |
| url | https://www.mdpi.com/2223-7747/10/2/248 |
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