Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.
<h4>Background</h4>Non-small cell lung cancer (NSCLC) harms human health, but its pathogenesis remains unclear. We wish to provide more molecular therapeutic targets for NSCLC.<h4>Methods</h4>The NSCLC tissue and normal tissue samples were screened for genetic comparison in t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0259091 |
| _version_ | 1798032918412525568 |
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| author | Kui Xiao Yang Wang Lihua Zhou Jufen Wang Yaohui Wang De Tong Zhiruo Zhu Jiehan Jiang |
| author_facet | Kui Xiao Yang Wang Lihua Zhou Jufen Wang Yaohui Wang De Tong Zhiruo Zhu Jiehan Jiang |
| author_sort | Kui Xiao |
| collection | DOAJ |
| description | <h4>Background</h4>Non-small cell lung cancer (NSCLC) harms human health, but its pathogenesis remains unclear. We wish to provide more molecular therapeutic targets for NSCLC.<h4>Methods</h4>The NSCLC tissue and normal tissue samples were screened for genetic comparison in the TCGA database. The predicted lncRNA and mRNA in BEAS2B and A549 cells were detected.<h4>Results</h4>Volcano plot displayed differentially expressed lncRNAs and mRNAs in adjacent tissues and NSCLC tissues. The survival curve showed that the lncRNA and mRNA had a significant impact on the patient's survival. The results of GO term enrichment analysis indicated that mRNA functions were enriched in cell cycle-related pathways. In the ceRNA interaction network, 13 lncRNAs and 20 miRNAs were found to have an interactive relationship. Finally, 3 significantly different lncRNAs (LINC00968, lnc-FAM92A-9 and lnc-PTGFR-1) and 6 mRNAs (CTCFL, KRT5, LY6D, TMEM, GBP6, and TMEM179) with potential therapeutic significance were screened out. And the cell experiment verified our results.<h4>Conclusion</h4>We screened out clinically significant 3 lncRNAs and 6 mRNAs involved in the ceRNA network, which were the key to our future research on the treatment of NSCLC. |
| first_indexed | 2024-04-11T20:21:51Z |
| format | Article |
| id | doaj.art-9b53889954b349748384cafeb32423b2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T20:21:51Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9b53889954b349748384cafeb32423b22022-12-22T04:04:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011610e025909110.1371/journal.pone.0259091Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.Kui XiaoYang WangLihua ZhouJufen WangYaohui WangDe TongZhiruo ZhuJiehan Jiang<h4>Background</h4>Non-small cell lung cancer (NSCLC) harms human health, but its pathogenesis remains unclear. We wish to provide more molecular therapeutic targets for NSCLC.<h4>Methods</h4>The NSCLC tissue and normal tissue samples were screened for genetic comparison in the TCGA database. The predicted lncRNA and mRNA in BEAS2B and A549 cells were detected.<h4>Results</h4>Volcano plot displayed differentially expressed lncRNAs and mRNAs in adjacent tissues and NSCLC tissues. The survival curve showed that the lncRNA and mRNA had a significant impact on the patient's survival. The results of GO term enrichment analysis indicated that mRNA functions were enriched in cell cycle-related pathways. In the ceRNA interaction network, 13 lncRNAs and 20 miRNAs were found to have an interactive relationship. Finally, 3 significantly different lncRNAs (LINC00968, lnc-FAM92A-9 and lnc-PTGFR-1) and 6 mRNAs (CTCFL, KRT5, LY6D, TMEM, GBP6, and TMEM179) with potential therapeutic significance were screened out. And the cell experiment verified our results.<h4>Conclusion</h4>We screened out clinically significant 3 lncRNAs and 6 mRNAs involved in the ceRNA network, which were the key to our future research on the treatment of NSCLC.https://doi.org/10.1371/journal.pone.0259091 |
| spellingShingle | Kui Xiao Yang Wang Lihua Zhou Jufen Wang Yaohui Wang De Tong Zhiruo Zhu Jiehan Jiang Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer. PLoS ONE |
| title | Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer. |
| title_full | Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer. |
| title_fullStr | Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer. |
| title_full_unstemmed | Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer. |
| title_short | Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer. |
| title_sort | construction of cerna network to identify the lncrna and mrna related to non small cell lung cancer |
| url | https://doi.org/10.1371/journal.pone.0259091 |
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