RESISTANCE LEVEL OF Pseudomonas stutzeri AGAINST MERCURY AND ITS ABILITY IN PRODUCTION OF MERCURY REDUCTASE ENZYME
Mercury reductase is an enzyme that is able to reduce Hg2+ to Hg0 non toxic. This enzyme is usually produced by mercury resistant bacteria. The research wanted to determine the resistance of indigenous Pseudomonas stutzeri isolate toward mercury and to explore the mercury reductase activity which is...
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| Format: | Article |
| Language: | English |
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Jenderal Soedirman University
2016-11-01
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| Series: | Molekul |
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| Online Access: | http://ojs.jmolekul.com/ojs/index.php/jm/article/view/256 |
| _version_ | 1819263387577614336 |
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| author | Purkan Purkan Safita Nurmalyya Sofijan Hadi |
| author_facet | Purkan Purkan Safita Nurmalyya Sofijan Hadi |
| author_sort | Purkan Purkan |
| collection | DOAJ |
| description | Mercury reductase is an enzyme that is able to reduce Hg2+ to Hg0 non toxic. This enzyme is usually produced by mercury resistant bacteria. The research wanted to determine the resistance of indigenous Pseudomonas stutzeri isolate toward mercury and to explore the mercury reductase activity which is produced by the bacteria. The results of resistance assay of the Pseudomonas stutzeri toward mercury ion showed that the isolate could survive in media containing HgCl2 up to a concentration of 80 µM. The bacteria could produce mercury reductase optimally at the 24th of fermentation time. The enzyme showed optimum activity at pH 7 and temperature of 45 oC |
| first_indexed | 2024-12-23T20:12:47Z |
| format | Article |
| id | doaj.art-9b565b7343a64507923d208cf9e40cac |
| institution | Directory Open Access Journal |
| issn | 1907-9761 2503-0310 |
| language | English |
| last_indexed | 2024-12-23T20:12:47Z |
| publishDate | 2016-11-01 |
| publisher | Jenderal Soedirman University |
| record_format | Article |
| series | Molekul |
| spelling | doaj.art-9b565b7343a64507923d208cf9e40cac2022-12-21T17:32:47ZengJenderal Soedirman UniversityMolekul1907-97612503-03102016-11-0111223023810.20884/1.jm.2016.11.2.256204RESISTANCE LEVEL OF Pseudomonas stutzeri AGAINST MERCURY AND ITS ABILITY IN PRODUCTION OF MERCURY REDUCTASE ENZYMEPurkan Purkan0Safita NurmalyyaSofijan HadiLaboratorium Biokimia, Departemen Kimia, Fakultas Sains dan Teknologi Universitas Airlangga, Jl. Mulyorejo, Surabaya 60115 IndonesiaMercury reductase is an enzyme that is able to reduce Hg2+ to Hg0 non toxic. This enzyme is usually produced by mercury resistant bacteria. The research wanted to determine the resistance of indigenous Pseudomonas stutzeri isolate toward mercury and to explore the mercury reductase activity which is produced by the bacteria. The results of resistance assay of the Pseudomonas stutzeri toward mercury ion showed that the isolate could survive in media containing HgCl2 up to a concentration of 80 µM. The bacteria could produce mercury reductase optimally at the 24th of fermentation time. The enzyme showed optimum activity at pH 7 and temperature of 45 oChttp://ojs.jmolekul.com/ojs/index.php/jm/article/view/256Pseudomonas stutzeri, mercury reductase, mercury |
| spellingShingle | Purkan Purkan Safita Nurmalyya Sofijan Hadi RESISTANCE LEVEL OF Pseudomonas stutzeri AGAINST MERCURY AND ITS ABILITY IN PRODUCTION OF MERCURY REDUCTASE ENZYME Molekul Pseudomonas stutzeri, mercury reductase, mercury |
| title | RESISTANCE LEVEL OF Pseudomonas stutzeri AGAINST MERCURY AND ITS ABILITY IN PRODUCTION OF MERCURY REDUCTASE ENZYME |
| title_full | RESISTANCE LEVEL OF Pseudomonas stutzeri AGAINST MERCURY AND ITS ABILITY IN PRODUCTION OF MERCURY REDUCTASE ENZYME |
| title_fullStr | RESISTANCE LEVEL OF Pseudomonas stutzeri AGAINST MERCURY AND ITS ABILITY IN PRODUCTION OF MERCURY REDUCTASE ENZYME |
| title_full_unstemmed | RESISTANCE LEVEL OF Pseudomonas stutzeri AGAINST MERCURY AND ITS ABILITY IN PRODUCTION OF MERCURY REDUCTASE ENZYME |
| title_short | RESISTANCE LEVEL OF Pseudomonas stutzeri AGAINST MERCURY AND ITS ABILITY IN PRODUCTION OF MERCURY REDUCTASE ENZYME |
| title_sort | resistance level of pseudomonas stutzeri against mercury and its ability in production of mercury reductase enzyme |
| topic | Pseudomonas stutzeri, mercury reductase, mercury |
| url | http://ojs.jmolekul.com/ojs/index.php/jm/article/view/256 |
| work_keys_str_mv | AT purkanpurkan resistancelevelofpseudomonasstutzeriagainstmercuryanditsabilityinproductionofmercuryreductaseenzyme AT safitanurmalyya resistancelevelofpseudomonasstutzeriagainstmercuryanditsabilityinproductionofmercuryreductaseenzyme AT sofijanhadi resistancelevelofpseudomonasstutzeriagainstmercuryanditsabilityinproductionofmercuryreductaseenzyme |