Endodontic Irrigants and Their Activation Efficacy on Cleansing Post-Space Root Canal Walls

The aim of this study was to evaluate the efficacy of activated irrigants (EDTA e NaOCL) during the cleansing of root walls, of the smear layer, of the debris, and gutta-percha after the preparation of the restorative space. Twenty single and multi-rooted (<i>n</i> = 20) have been collec...

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Main Authors: Manuela Marchese, Pontoriero I. K. Denise, Edoardo Ferrari Cagidiaco, Alfredo Iandolo, Simone Grandini, Marco Ferrari
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Prosthesis
Subjects:
Online Access:https://www.mdpi.com/2673-1592/3/4/36
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author Manuela Marchese
Pontoriero I. K. Denise
Edoardo Ferrari Cagidiaco
Alfredo Iandolo
Simone Grandini
Marco Ferrari
author_facet Manuela Marchese
Pontoriero I. K. Denise
Edoardo Ferrari Cagidiaco
Alfredo Iandolo
Simone Grandini
Marco Ferrari
author_sort Manuela Marchese
collection DOAJ
description The aim of this study was to evaluate the efficacy of activated irrigants (EDTA e NaOCL) during the cleansing of root walls, of the smear layer, of the debris, and gutta-percha after the preparation of the restorative space. Twenty single and multi-rooted (<i>n</i> = 20) have been collected. All samples were prepared by the same operator, using Nickel-titanium rotating instruments (Mtwo) through the Simultaneous Shaping Technique. The continuous-wave of condensation technique of obturation was used. To all specimens, the restorative space has been made, leaving 5 mm of apical gutta-percha, and postoperative periapical X-rays were performed. The samples were randomly divided into two groups: Group (A): cleansing of the root walls with ultrasonic activation of the irrigants (NEWTRON P5 XS; Satelec Acteon); Group (B): radicular walls wash without ultrasonic activation of endodontic irrigants (NaOCl 5.25% and EDTA 17%). Both dental sample groups were cut longitudinally with a low-speed saw (Isomet); the samples were observed by using a scanning electron microscope (Jeol, Jsm-6060LV) in order to evaluate: (1) the amount of debris/smear layer; (2) the mount of obstruction of dentinal tubules found in the two groups; and (3) evaluation of the presence of gutta-percha. Then, the other five samples each group (with and without ultrasonic activation) were prepared following the same protocol. Then, a universal bonding system (G-Praemio Bond, GC) and a layer of a flowable resin composite (Gaenial Flow, GC) were light-cured and used on top of the prepared root canal walls. The samples were cut in two pieces along the long axis of the root. Then, half sample teeth were kept in an acidic solution (37% HCl) for 48 h in order to completely dissolve dental structures and to have a direct view of resin tags formation under SEM. The other half was prepared to observe the adhesive interface under SEM. The amount of debris was not satisfactory in 9 out of 10 cases in Group B, while in Group A, which has been treated with ultrasounds, the result was either good or great in most of the samples. For the sample group treated with ultrasound, the tubules were evaluated as perfectly clean in 9 out of 10 cases, instead, the results are unsatisfactory for 9 out of 10 cases of group B not treated with ultrasound. Differences between Group A and B were statistically significant. With respect to the presence of debris and tubules obstruction treatment with ultrasonic activation, it offers with no doubt better results. When ultrasonic activation is used in combination with endodontic irrigants, a clean dentin substrate is be obtained for the adhesion of restorative materials, but in order to confirm the findings of this study, further in vivo trials are needed.
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spelling doaj.art-9b58083d60754e729302f1eea13eca362023-11-23T10:19:55ZengMDPI AGProsthesis2673-15922021-12-013440641410.3390/prosthesis3040036Endodontic Irrigants and Their Activation Efficacy on Cleansing Post-Space Root Canal WallsManuela Marchese0Pontoriero I. K. Denise1Edoardo Ferrari Cagidiaco2Alfredo Iandolo3Simone Grandini4Marco Ferrari5Department of Prosthodontics and Dental Materials, University of Siena, 53100 Siena, ItalyDepartment of Prosthodontics and Dental Materials, University of Siena, 53100 Siena, ItalyDepartment of Prosthodontics and Dental Materials, University of Siena, 53100 Siena, ItalyDepartment of Endodontics, University of Salerno, 84084 Fisciano, ItalyDepartment of Endodontics and Restorative Dentistry, University of Siena, 53100 Siena, ItalyDepartment of Prosthodontics and Dental Materials, University of Siena, 53100 Siena, ItalyThe aim of this study was to evaluate the efficacy of activated irrigants (EDTA e NaOCL) during the cleansing of root walls, of the smear layer, of the debris, and gutta-percha after the preparation of the restorative space. Twenty single and multi-rooted (<i>n</i> = 20) have been collected. All samples were prepared by the same operator, using Nickel-titanium rotating instruments (Mtwo) through the Simultaneous Shaping Technique. The continuous-wave of condensation technique of obturation was used. To all specimens, the restorative space has been made, leaving 5 mm of apical gutta-percha, and postoperative periapical X-rays were performed. The samples were randomly divided into two groups: Group (A): cleansing of the root walls with ultrasonic activation of the irrigants (NEWTRON P5 XS; Satelec Acteon); Group (B): radicular walls wash without ultrasonic activation of endodontic irrigants (NaOCl 5.25% and EDTA 17%). Both dental sample groups were cut longitudinally with a low-speed saw (Isomet); the samples were observed by using a scanning electron microscope (Jeol, Jsm-6060LV) in order to evaluate: (1) the amount of debris/smear layer; (2) the mount of obstruction of dentinal tubules found in the two groups; and (3) evaluation of the presence of gutta-percha. Then, the other five samples each group (with and without ultrasonic activation) were prepared following the same protocol. Then, a universal bonding system (G-Praemio Bond, GC) and a layer of a flowable resin composite (Gaenial Flow, GC) were light-cured and used on top of the prepared root canal walls. The samples were cut in two pieces along the long axis of the root. Then, half sample teeth were kept in an acidic solution (37% HCl) for 48 h in order to completely dissolve dental structures and to have a direct view of resin tags formation under SEM. The other half was prepared to observe the adhesive interface under SEM. The amount of debris was not satisfactory in 9 out of 10 cases in Group B, while in Group A, which has been treated with ultrasounds, the result was either good or great in most of the samples. For the sample group treated with ultrasound, the tubules were evaluated as perfectly clean in 9 out of 10 cases, instead, the results are unsatisfactory for 9 out of 10 cases of group B not treated with ultrasound. Differences between Group A and B were statistically significant. With respect to the presence of debris and tubules obstruction treatment with ultrasonic activation, it offers with no doubt better results. When ultrasonic activation is used in combination with endodontic irrigants, a clean dentin substrate is be obtained for the adhesion of restorative materials, but in order to confirm the findings of this study, further in vivo trials are needed.https://www.mdpi.com/2673-1592/3/4/36post spacecleaning root canalultrasonic activation
spellingShingle Manuela Marchese
Pontoriero I. K. Denise
Edoardo Ferrari Cagidiaco
Alfredo Iandolo
Simone Grandini
Marco Ferrari
Endodontic Irrigants and Their Activation Efficacy on Cleansing Post-Space Root Canal Walls
Prosthesis
post space
cleaning root canal
ultrasonic activation
title Endodontic Irrigants and Their Activation Efficacy on Cleansing Post-Space Root Canal Walls
title_full Endodontic Irrigants and Their Activation Efficacy on Cleansing Post-Space Root Canal Walls
title_fullStr Endodontic Irrigants and Their Activation Efficacy on Cleansing Post-Space Root Canal Walls
title_full_unstemmed Endodontic Irrigants and Their Activation Efficacy on Cleansing Post-Space Root Canal Walls
title_short Endodontic Irrigants and Their Activation Efficacy on Cleansing Post-Space Root Canal Walls
title_sort endodontic irrigants and their activation efficacy on cleansing post space root canal walls
topic post space
cleaning root canal
ultrasonic activation
url https://www.mdpi.com/2673-1592/3/4/36
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AT alfredoiandolo endodonticirrigantsandtheiractivationefficacyoncleansingpostspacerootcanalwalls
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