An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng
IntroductionRadix Notoginseng, one of the most famous Chinese traditional medicines, is the dried root of Panax notoginseng (Araliaceae). Stems and leaves of P. notoginseng (SLPN) are rich in secondary metabolites and nutrients, and authorized as a food resource, however, its utilization needs furth...
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1058639/full |
| _version_ | 1811185352519450624 |
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| author | Zhengwei Liang Zhengwei Liang Zhengwei Liang Kunyi Liu Kunyi Liu Kunyi Liu Ruoyu Li Ruoyu Li Ruoyu Li Baiping Ma Wei Zheng Shengchao Yang Shengchao Yang Shengchao Yang Guanghui Zhang Guanghui Zhang Guanghui Zhang Yinhe Zhao Junwen Chen Junwen Chen Junwen Chen Ming Zhao Ming Zhao Ming Zhao |
| author_facet | Zhengwei Liang Zhengwei Liang Zhengwei Liang Kunyi Liu Kunyi Liu Kunyi Liu Ruoyu Li Ruoyu Li Ruoyu Li Baiping Ma Wei Zheng Shengchao Yang Shengchao Yang Shengchao Yang Guanghui Zhang Guanghui Zhang Guanghui Zhang Yinhe Zhao Junwen Chen Junwen Chen Junwen Chen Ming Zhao Ming Zhao Ming Zhao |
| author_sort | Zhengwei Liang |
| collection | DOAJ |
| description | IntroductionRadix Notoginseng, one of the most famous Chinese traditional medicines, is the dried root of Panax notoginseng (Araliaceae). Stems and leaves of P. notoginseng (SLPN) are rich in secondary metabolites and nutrients, and authorized as a food resource, however, its utilization needs further research.MethodsA SLPN-instant beverage was manufactured from SLPN through optimization by response surface design with 21-fold of 48.50% ethanol for 39 h, and this extraction was repeated twice; the extraction solution was concentrated to 1/3 volume using a vacuum rotatory evaporator at 45°C, and then spray dried at 110°C. Nutritional components including 14 amino acids, ten mineral elements, 15 vitamins were detected in the SLPN-instant beverage; forty-three triterpenoid saponins, e.g., ginsenoside La, ginsenoside Rb3, notoginsenoside R1, and two flavonoid glycosides, as well as dencichine were identified by UPLC-MS.ResultsThe extraction rate of SLPN-instant beverage was 37.89 ± 0.02%. The majority nutrients were Gly (2.10 ± 0.63 mg/g), His (1.23 ± 0.07 mg/g), α-VE (18.89 ± 1.87 μg/g), β-VE (17.53 ± 1.98 μg/g), potassium (49.26 ± 2.70 mg/g), calcium (6.73 ± 0.27 mg/g). The total saponin of the SLPN-instant beverage was 403.05 ± 34.98 mg/g, majority was notoginsenoside Fd and with contents of 227 ± 2.02 mg/g. In addition, catechin and γ-aminobutyric acid were detected with levels of 24.57 ± 0.21 mg/g and 7.50 ± 1.85 mg/g, respectively. The SLPN-instant beverage showed good antioxidant activities with half maximal inhibitory concentration (IC50) for scavenging hydroxyl (OH–) radicals, superoxide anion (O2–) radicals, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS+) radicals were 0.1954, 0.2314, 0.4083, and 0.3874 mg/mL, respectively.ConclusionWe optimized an analytical method for in depth analysis of the newly authorized food resource SLPN. Together, an instant beverage with antioxidant activity, rich in nutrients and secondary metabolites, was manufactured from SLPN, which may improve the utilization of SLPN. |
| first_indexed | 2024-04-11T13:29:10Z |
| format | Article |
| id | doaj.art-9b5912f6de8f4408901ccebd8cfb3e34 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-04-11T13:29:10Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Nutrition |
| spelling | doaj.art-9b5912f6de8f4408901ccebd8cfb3e342022-12-22T04:21:56ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-12-01910.3389/fnut.2022.10586391058639An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginsengZhengwei Liang0Zhengwei Liang1Zhengwei Liang2Kunyi Liu3Kunyi Liu4Kunyi Liu5Ruoyu Li6Ruoyu Li7Ruoyu Li8Baiping Ma9Wei Zheng10Shengchao Yang11Shengchao Yang12Shengchao Yang13Guanghui Zhang14Guanghui Zhang15Guanghui Zhang16Yinhe Zhao17Junwen Chen18Junwen Chen19Junwen Chen20Ming Zhao21Ming Zhao22Ming Zhao23College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, ChinaYunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, ChinaThe Key Laboratory of Medicinal Plant Biology of Yunnan Province, National Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwestern, Kunming, Yunnan, ChinaYunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, ChinaThe Key Laboratory of Medicinal Plant Biology of Yunnan Province, National Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwestern, Kunming, Yunnan, ChinaCollege of Wuliangye Technology and Food Engineering, Yibin Vocational and Technical College, Yibin, Sichuan, ChinaYunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, ChinaThe Key Laboratory of Medicinal Plant Biology of Yunnan Province, National Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwestern, Kunming, Yunnan, ChinaCollege of Tea Science, Yunnan Agricultural University, Kunming, Yunnan, ChinaBeijing Institute of Radiation Medicine, Beijing, ChinaBeijing Institute of Radiation Medicine, Beijing, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, ChinaYunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, ChinaThe Key Laboratory of Medicinal Plant Biology of Yunnan Province, National Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwestern, Kunming, Yunnan, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, ChinaYunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, ChinaThe Key Laboratory of Medicinal Plant Biology of Yunnan Province, National Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwestern, Kunming, Yunnan, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, ChinaYunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, ChinaThe Key Laboratory of Medicinal Plant Biology of Yunnan Province, National Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwestern, Kunming, Yunnan, ChinaYunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, ChinaThe Key Laboratory of Medicinal Plant Biology of Yunnan Province, National Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwestern, Kunming, Yunnan, ChinaCollege of Tea Science, Yunnan Agricultural University, Kunming, Yunnan, ChinaIntroductionRadix Notoginseng, one of the most famous Chinese traditional medicines, is the dried root of Panax notoginseng (Araliaceae). Stems and leaves of P. notoginseng (SLPN) are rich in secondary metabolites and nutrients, and authorized as a food resource, however, its utilization needs further research.MethodsA SLPN-instant beverage was manufactured from SLPN through optimization by response surface design with 21-fold of 48.50% ethanol for 39 h, and this extraction was repeated twice; the extraction solution was concentrated to 1/3 volume using a vacuum rotatory evaporator at 45°C, and then spray dried at 110°C. Nutritional components including 14 amino acids, ten mineral elements, 15 vitamins were detected in the SLPN-instant beverage; forty-three triterpenoid saponins, e.g., ginsenoside La, ginsenoside Rb3, notoginsenoside R1, and two flavonoid glycosides, as well as dencichine were identified by UPLC-MS.ResultsThe extraction rate of SLPN-instant beverage was 37.89 ± 0.02%. The majority nutrients were Gly (2.10 ± 0.63 mg/g), His (1.23 ± 0.07 mg/g), α-VE (18.89 ± 1.87 μg/g), β-VE (17.53 ± 1.98 μg/g), potassium (49.26 ± 2.70 mg/g), calcium (6.73 ± 0.27 mg/g). The total saponin of the SLPN-instant beverage was 403.05 ± 34.98 mg/g, majority was notoginsenoside Fd and with contents of 227 ± 2.02 mg/g. In addition, catechin and γ-aminobutyric acid were detected with levels of 24.57 ± 0.21 mg/g and 7.50 ± 1.85 mg/g, respectively. The SLPN-instant beverage showed good antioxidant activities with half maximal inhibitory concentration (IC50) for scavenging hydroxyl (OH–) radicals, superoxide anion (O2–) radicals, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS+) radicals were 0.1954, 0.2314, 0.4083, and 0.3874 mg/mL, respectively.ConclusionWe optimized an analytical method for in depth analysis of the newly authorized food resource SLPN. Together, an instant beverage with antioxidant activity, rich in nutrients and secondary metabolites, was manufactured from SLPN, which may improve the utilization of SLPN.https://www.frontiersin.org/articles/10.3389/fnut.2022.1058639/fullantioxidantnutrientsPanax notoginsengpolyphenolsaponin |
| spellingShingle | Zhengwei Liang Zhengwei Liang Zhengwei Liang Kunyi Liu Kunyi Liu Kunyi Liu Ruoyu Li Ruoyu Li Ruoyu Li Baiping Ma Wei Zheng Shengchao Yang Shengchao Yang Shengchao Yang Guanghui Zhang Guanghui Zhang Guanghui Zhang Yinhe Zhao Junwen Chen Junwen Chen Junwen Chen Ming Zhao Ming Zhao Ming Zhao An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng Frontiers in Nutrition antioxidant nutrients Panax notoginseng polyphenol saponin |
| title | An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng |
| title_full | An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng |
| title_fullStr | An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng |
| title_full_unstemmed | An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng |
| title_short | An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng |
| title_sort | instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of panax notoginseng |
| topic | antioxidant nutrients Panax notoginseng polyphenol saponin |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1058639/full |
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