The effect of mild agonist stimulation on the platelet reactivity in patients with type 2 diabetes mellitus
Abstract Background Patients with type 2 diabetes mellitus (T2DM) have accelerated atherosclerosis as a pro thrombotic state that is associated with the platelet activation priming. Platelets, which undergo the continuous mild stimulation, may lose their sensitivity to react to a strong stimulation....
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | BMC Endocrine Disorders |
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| Online Access: | http://link.springer.com/article/10.1186/s12902-019-0391-2 |
| _version_ | 1818336497844092928 |
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| author | Razie Mahmoodian Morteza Salimian Mohsen Hamidpour Ali Akbar Khadem-Maboudi Ahmad Gharehbaghian |
| author_facet | Razie Mahmoodian Morteza Salimian Mohsen Hamidpour Ali Akbar Khadem-Maboudi Ahmad Gharehbaghian |
| author_sort | Razie Mahmoodian |
| collection | DOAJ |
| description | Abstract Background Patients with type 2 diabetes mellitus (T2DM) have accelerated atherosclerosis as a pro thrombotic state that is associated with the platelet activation priming. Platelets, which undergo the continuous mild stimulation, may lose their sensitivity to react to a strong stimulation. The present study aimed to investigate activation responses of platelets to mild and subsequent strong stimulations in patients with T2DM and healthy individuals. Methods Blood samples, which were taken from 40 patients with T2DM and 35 healthy individuals, were collected into the citrate containing tubes. The samples were subjected to the soft centrifugation to prepare the platelet rich plasma (PRP). Platelets in PRP samples were treated at a low (1 μM) concentration and then at a high (10 μM) concentration of ADP. Before and after stimulation with different doses of ADP, levels of CD62P expression and formation of platelet micro particles (PMPs) were measured using a flow cytometry method. Results The platelets from patients with T2DM had higher levels of CD62P expression before any stimulation (P = 0.003) than control samples. Platelets, which underwent the mild stimulation, indicated lower responses to CD62P expression, but higher PMPs formation after stimulation with high dose of ADP. Patients with T2DM had higher platelet micro particles in all states with the ADP stimulation. (P = 0.004, SD: ±74.52). Conclusions The flow cytometry data indicated that platelets were pre-active and associated with metabolic conditions in patients with type 2 diabetes mellitus. The induction of desensitization state helped platelets to reduce the platelet activation and sensitivity to ADP in a diabetic environment. Furthermore, the production of platelets micro-particles was high in the patients; and desensitized platelets were more susceptible to shedding of micro-particles. |
| first_indexed | 2024-12-13T14:40:16Z |
| format | Article |
| id | doaj.art-9b597c9d2bb143d7b0dbb8e58713306d |
| institution | Directory Open Access Journal |
| issn | 1472-6823 |
| language | English |
| last_indexed | 2024-12-13T14:40:16Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Endocrine Disorders |
| spelling | doaj.art-9b597c9d2bb143d7b0dbb8e58713306d2022-12-21T23:41:38ZengBMCBMC Endocrine Disorders1472-68232019-06-011911810.1186/s12902-019-0391-2The effect of mild agonist stimulation on the platelet reactivity in patients with type 2 diabetes mellitusRazie Mahmoodian0Morteza Salimian1Mohsen Hamidpour2Ali Akbar Khadem-Maboudi3Ahmad Gharehbaghian4Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical SciencesParamedical Faculty, Kashan University of Medical SciencesDepartment of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical SciencesDepartment of Bio statistical, School of Paramedical Sciences, Shahid Beheshti University of Medical SciencesDepartment of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical SciencesAbstract Background Patients with type 2 diabetes mellitus (T2DM) have accelerated atherosclerosis as a pro thrombotic state that is associated with the platelet activation priming. Platelets, which undergo the continuous mild stimulation, may lose their sensitivity to react to a strong stimulation. The present study aimed to investigate activation responses of platelets to mild and subsequent strong stimulations in patients with T2DM and healthy individuals. Methods Blood samples, which were taken from 40 patients with T2DM and 35 healthy individuals, were collected into the citrate containing tubes. The samples were subjected to the soft centrifugation to prepare the platelet rich plasma (PRP). Platelets in PRP samples were treated at a low (1 μM) concentration and then at a high (10 μM) concentration of ADP. Before and after stimulation with different doses of ADP, levels of CD62P expression and formation of platelet micro particles (PMPs) were measured using a flow cytometry method. Results The platelets from patients with T2DM had higher levels of CD62P expression before any stimulation (P = 0.003) than control samples. Platelets, which underwent the mild stimulation, indicated lower responses to CD62P expression, but higher PMPs formation after stimulation with high dose of ADP. Patients with T2DM had higher platelet micro particles in all states with the ADP stimulation. (P = 0.004, SD: ±74.52). Conclusions The flow cytometry data indicated that platelets were pre-active and associated with metabolic conditions in patients with type 2 diabetes mellitus. The induction of desensitization state helped platelets to reduce the platelet activation and sensitivity to ADP in a diabetic environment. Furthermore, the production of platelets micro-particles was high in the patients; and desensitized platelets were more susceptible to shedding of micro-particles.http://link.springer.com/article/10.1186/s12902-019-0391-2Type 2 diabetesDesensitizationAdenosine diphosphate (ADP)Platelets |
| spellingShingle | Razie Mahmoodian Morteza Salimian Mohsen Hamidpour Ali Akbar Khadem-Maboudi Ahmad Gharehbaghian The effect of mild agonist stimulation on the platelet reactivity in patients with type 2 diabetes mellitus BMC Endocrine Disorders Type 2 diabetes Desensitization Adenosine diphosphate (ADP) Platelets |
| title | The effect of mild agonist stimulation on the platelet reactivity in patients with type 2 diabetes mellitus |
| title_full | The effect of mild agonist stimulation on the platelet reactivity in patients with type 2 diabetes mellitus |
| title_fullStr | The effect of mild agonist stimulation on the platelet reactivity in patients with type 2 diabetes mellitus |
| title_full_unstemmed | The effect of mild agonist stimulation on the platelet reactivity in patients with type 2 diabetes mellitus |
| title_short | The effect of mild agonist stimulation on the platelet reactivity in patients with type 2 diabetes mellitus |
| title_sort | effect of mild agonist stimulation on the platelet reactivity in patients with type 2 diabetes mellitus |
| topic | Type 2 diabetes Desensitization Adenosine diphosphate (ADP) Platelets |
| url | http://link.springer.com/article/10.1186/s12902-019-0391-2 |
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