Methylation Status of miR-182 Promoter in Lung Cancer Cell Lines
Background and objective It has been proven that the abnormal expression of miR-182 was related to the occurrence and development of tumors. The aim of this study is to explore the relationship between the methylation of miR-182 promoter and its expression in lung cancer cell lines. Methods Real-tim...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2015-05-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2015.05.02 |
| _version_ | 1819294104990777344 |
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| author | Yongwen LI Yonglin SUN Fan REN Ying LI Minghui LIU Hongyu LIU Jun CHEN |
| author_facet | Yongwen LI Yonglin SUN Fan REN Ying LI Minghui LIU Hongyu LIU Jun CHEN |
| author_sort | Yongwen LI |
| collection | DOAJ |
| description | Background and objective It has been proven that the abnormal expression of miR-182 was related to the occurrence and development of tumors. The aim of this study is to explore the relationship between the methylation of miR-182 promoter and its expression in lung cancer cell lines. Methods Real-time quantitative PCR and methylation-specific PCR were used to detect the expression level of miR-182 and its promoter methylation status in five lung cancer cell lines (A549, L9981, NL9980, 95C and 95D). DNA sequencing was used to confirm the methylation results. Results The level of miR-182 expression significantly differs among these lung cancer cell lines. The highly metastatic human lung cancer cell lines, namely, A549 and L9981, demonstrate a relatively lower expression level of miR-182 compared with the lowly metastatic human lung cancer cell line 95C. Methylation-specific PCR and DNA sequencing assay results indicate that these lung cancer cell lines present different levels of miR-182 promoter methylation, and the highest methylation level is observed in A549 cells. Furthermore, the expression of miR-182 in these cell lines significantly increases when treated with 10 μM 5’-Aza-dC. Conclusion DNA methylation occurs in the miR-182 promoter region in lung cancer cell lines. This methylation can regulate the expression level of miR-182. Further study must be conducted to explore the function of miR-182 promoter methylation in lung cancer occurrence and development. |
| first_indexed | 2024-12-24T04:21:01Z |
| format | Article |
| id | doaj.art-9b61bdd8cff74b75ba3f08674304474d |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-12-24T04:21:01Z |
| publishDate | 2015-05-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-9b61bdd8cff74b75ba3f08674304474d2022-12-21T17:15:49ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872015-05-0118526026510.3779/j.issn.1009-3419.2015.05.02Methylation Status of miR-182 Promoter in Lung Cancer Cell LinesYongwen LI0Yonglin SUN1Fan REN2Ying LI3Minghui LIU4Hongyu LIU5Jun CHEN6Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital,
Tianjin 300052, ChinaDepartment of Lung Cancer Surgery, Tianjin Medical University General Hospital,
Tianjin 300052, ChinaDepartment of Lung Cancer Surgery, Tianjin Medical University General Hospital,
Tianjin 300052, ChinaTianjin Lung Cancer Institute, Tianjin Medical University General Hospital,
Tianjin 300052, ChinaDepartment of Lung Cancer Surgery, Tianjin Medical University General Hospital,
Tianjin 300052, ChinaTianjin Lung Cancer Institute, Tianjin Medical University General Hospital,
Tianjin 300052, ChinaTianjin Lung Cancer Institute, Tianjin Medical University General Hospital,
Tianjin 300052, ChinaBackground and objective It has been proven that the abnormal expression of miR-182 was related to the occurrence and development of tumors. The aim of this study is to explore the relationship between the methylation of miR-182 promoter and its expression in lung cancer cell lines. Methods Real-time quantitative PCR and methylation-specific PCR were used to detect the expression level of miR-182 and its promoter methylation status in five lung cancer cell lines (A549, L9981, NL9980, 95C and 95D). DNA sequencing was used to confirm the methylation results. Results The level of miR-182 expression significantly differs among these lung cancer cell lines. The highly metastatic human lung cancer cell lines, namely, A549 and L9981, demonstrate a relatively lower expression level of miR-182 compared with the lowly metastatic human lung cancer cell line 95C. Methylation-specific PCR and DNA sequencing assay results indicate that these lung cancer cell lines present different levels of miR-182 promoter methylation, and the highest methylation level is observed in A549 cells. Furthermore, the expression of miR-182 in these cell lines significantly increases when treated with 10 μM 5’-Aza-dC. Conclusion DNA methylation occurs in the miR-182 promoter region in lung cancer cell lines. This methylation can regulate the expression level of miR-182. Further study must be conducted to explore the function of miR-182 promoter methylation in lung cancer occurrence and development.http://dx.doi.org/10.3779/j.issn.1009-3419.2015.05.02DNA methylationmiR-182Lung neoplasms |
| spellingShingle | Yongwen LI Yonglin SUN Fan REN Ying LI Minghui LIU Hongyu LIU Jun CHEN Methylation Status of miR-182 Promoter in Lung Cancer Cell Lines Chinese Journal of Lung Cancer DNA methylation miR-182 Lung neoplasms |
| title | Methylation Status of miR-182 Promoter in Lung Cancer Cell Lines |
| title_full | Methylation Status of miR-182 Promoter in Lung Cancer Cell Lines |
| title_fullStr | Methylation Status of miR-182 Promoter in Lung Cancer Cell Lines |
| title_full_unstemmed | Methylation Status of miR-182 Promoter in Lung Cancer Cell Lines |
| title_short | Methylation Status of miR-182 Promoter in Lung Cancer Cell Lines |
| title_sort | methylation status of mir 182 promoter in lung cancer cell lines |
| topic | DNA methylation miR-182 Lung neoplasms |
| url | http://dx.doi.org/10.3779/j.issn.1009-3419.2015.05.02 |
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