Phytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation
Phytosulfokine (PSK) is a tyrosine-sulfated peptide that is widely distributed in plants, participating in cell proliferation, differentiation, and innate immunity. The potential role of PSK in nodulation in legumes has not been reported. In this work, five PSK precursor genes were identified in Lot...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2015-08-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-02-15-0032-R |
| _version_ | 1819081483120279552 |
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| author | Chao Wang Haixiang Yu Zhongming Zhang Liangliang Yu Xiaoshu Xu Zonglie Hong Li Luo |
| author_facet | Chao Wang Haixiang Yu Zhongming Zhang Liangliang Yu Xiaoshu Xu Zonglie Hong Li Luo |
| author_sort | Chao Wang |
| collection | DOAJ |
| description | Phytosulfokine (PSK) is a tyrosine-sulfated peptide that is widely distributed in plants, participating in cell proliferation, differentiation, and innate immunity. The potential role of PSK in nodulation in legumes has not been reported. In this work, five PSK precursor genes were identified in Lotus japonicas, designated as LjPSK1 to LjPSK5. Three of them (LjPSK1, LjPSK4, and LjPSK5) were found to be expressed in nitrogen-fixing root nodules. LjPSK1 and LjPSK4 were not induced at the early stage of nodulation. Interestingly, while the expression of LjPSK4 was also found in spontaneous nodules without rhizobial colonization, LjPSK1 was not induced in these pseudo nodules. Promoter-β-glucuronidase analysis revealed that LjPSK1 was highly expressed in enlarged symbiotic cells of nodules. Exogenous addition of 1 μM synthetic PSK peptide resulted in increased nodule numbers per plant. Consistently, the number of mature nodules but not the events of rhizobial infection and nodule initiation was increased by overexpressing LjPSK1 in transgenic hairy roots, in which the expression of jasmonate-responsive genes was found to be repressed. These results suggest that PSK is a new peptide signal that regulates nodulation in legumes, probably through cross-talking with other phytohormones. |
| first_indexed | 2024-12-21T20:01:29Z |
| format | Article |
| id | doaj.art-9b61dd1f46084f9dbe17c939dd3ff75d |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-21T20:01:29Z |
| publishDate | 2015-08-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-9b61dd1f46084f9dbe17c939dd3ff75d2022-12-21T18:51:58ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062015-08-0128884785510.1094/MPMI-02-15-0032-RPhytosulfokine Is Involved in Positive Regulation of Lotus japonicus NodulationChao Wang0Haixiang Yu1Zhongming Zhang2Liangliang Yu3Xiaoshu Xu4Zonglie Hong5Li Luo6Shanghai Key Lab of Bio-energy Crops, School of Life Sciences, Shanghai University, Shanghai, 200444, China;State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;Shanghai Key Lab of Bio-energy Crops, School of Life Sciences, Shanghai University, Shanghai, 200444, China;State Key Lab of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China;Department of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844, U.S.A.Shanghai Key Lab of Bio-energy Crops, School of Life Sciences, Shanghai University, Shanghai, 200444, China;Phytosulfokine (PSK) is a tyrosine-sulfated peptide that is widely distributed in plants, participating in cell proliferation, differentiation, and innate immunity. The potential role of PSK in nodulation in legumes has not been reported. In this work, five PSK precursor genes were identified in Lotus japonicas, designated as LjPSK1 to LjPSK5. Three of them (LjPSK1, LjPSK4, and LjPSK5) were found to be expressed in nitrogen-fixing root nodules. LjPSK1 and LjPSK4 were not induced at the early stage of nodulation. Interestingly, while the expression of LjPSK4 was also found in spontaneous nodules without rhizobial colonization, LjPSK1 was not induced in these pseudo nodules. Promoter-β-glucuronidase analysis revealed that LjPSK1 was highly expressed in enlarged symbiotic cells of nodules. Exogenous addition of 1 μM synthetic PSK peptide resulted in increased nodule numbers per plant. Consistently, the number of mature nodules but not the events of rhizobial infection and nodule initiation was increased by overexpressing LjPSK1 in transgenic hairy roots, in which the expression of jasmonate-responsive genes was found to be repressed. These results suggest that PSK is a new peptide signal that regulates nodulation in legumes, probably through cross-talking with other phytohormones.https://apsjournals.apsnet.org/doi/10.1094/MPMI-02-15-0032-R |
| spellingShingle | Chao Wang Haixiang Yu Zhongming Zhang Liangliang Yu Xiaoshu Xu Zonglie Hong Li Luo Phytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation Molecular Plant-Microbe Interactions |
| title | Phytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation |
| title_full | Phytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation |
| title_fullStr | Phytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation |
| title_full_unstemmed | Phytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation |
| title_short | Phytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation |
| title_sort | phytosulfokine is involved in positive regulation of lotus japonicus nodulation |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-02-15-0032-R |
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