Subclinical Mastitis in Selected Bovine Dairy Herds in North Upper Egypt: Assessment of Prevalence, Causative Bacterial Pathogens, Antimicrobial Resistance and Virulence-Associated Genes
Mastitis is a significant disease affecting dairy cattle farms in Egypt. The current study aimed to investigate the prevalence and major bacterial pathogens causing subclinical mastitis (SCM) in three bovine dairy herds, with a history of SCM, at three Governorates in North Upper Egypt. The antimicr...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/6/1175 |
| _version_ | 1827691146861608960 |
|---|---|
| author | Ahmed H. Abed Ahmed M. S. Menshawy Mohamed M. A. Zeinhom Delower Hossain Eman Khalifa Gamal Wareth Mohamed F. Awad |
| author_facet | Ahmed H. Abed Ahmed M. S. Menshawy Mohamed M. A. Zeinhom Delower Hossain Eman Khalifa Gamal Wareth Mohamed F. Awad |
| author_sort | Ahmed H. Abed |
| collection | DOAJ |
| description | Mastitis is a significant disease affecting dairy cattle farms in Egypt. The current study aimed to investigate the prevalence and major bacterial pathogens causing subclinical mastitis (SCM) in three bovine dairy herds, with a history of SCM, at three Governorates in North Upper Egypt. The antimicrobial resistance profiles and specific virulence-associated genes causing bovine SCM were investigated. One thousand sixty-quarter milk samples (QMS) were collected aseptically from 270 apparently healthy cows in three farms and examined. The total prevalence of SCM was 46% and 44.8% based on California Mastitis Test (CMT) and Somatic Cell Count (SCC), respectively. Bacteriological examination of CMT positive quarters revealed that the prevalence of bacterial isolation in subclinically mastitic quarters was 90.4% (26 and 64.3% had single and mixed isolates, respectively). The most frequent bacterial isolates were <i>E. coli</i> (49.8%), <i>Staphylococcus aureus</i> (44.9%), streptococci (44.1%) and non-<i>aureus</i> staphylococci (NAS) (37.1%). Antimicrobial susceptibility testing of isolates revealed a high degree of resistance to the most commonly used antimicrobial compound in human and veterinary medicine. Implementation of PCR revealed the presence of <i>mec</i>A and <i>bla</i>Z genes in 60% and 46.7% of <i>S. aureus</i> isolates and in 26.7% and 53.3% of NAS, respectively. Meanwhile 73.3% of streptococci isolates harbored <i>aph</i>(3’)-IIIa gene conferring resistance to aminoglycosides and <i>cfb</i> gene. All <i>E. coli</i> isolates harbored <i>tet</i>A gene conferring resistance to tetracycline and <i>sul</i>1 gene conferring resistance to sulfonamides. The <i>fim</i>H and <i>tsh</i> genes were found in 80% and 60%, respectively. A significant association between the phenotypes and genotypes of AMR in different bacteria was recorded. The presence of a high prevalence of SCM in dairy animals impacts milk production and milk quality. The coexistence of pathogenic bacteria in milk is alarming, threatens human health and has a public health significance. Herd health improvement interventions are required to protect human health and society. |
| first_indexed | 2024-03-10T10:53:50Z |
| format | Article |
| id | doaj.art-9b644ad9d36a48c1851a5ccda22bcdc6 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T10:53:50Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-9b644ad9d36a48c1851a5ccda22bcdc62023-11-21T22:02:12ZengMDPI AGMicroorganisms2076-26072021-05-0196117510.3390/microorganisms9061175Subclinical Mastitis in Selected Bovine Dairy Herds in North Upper Egypt: Assessment of Prevalence, Causative Bacterial Pathogens, Antimicrobial Resistance and Virulence-Associated GenesAhmed H. Abed0Ahmed M. S. Menshawy1Mohamed M. A. Zeinhom2Delower Hossain3Eman Khalifa4Gamal Wareth5Mohamed F. Awad6Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Veterinary Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, EgyptFood Hygiene and Control Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Medicine and Public Health, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, BangladeshDepartment of Microbiology, Faculty of Veterinary Medicine, Matrouh University, Matrouh 51511, EgyptFriedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, GermanyDepartment of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaMastitis is a significant disease affecting dairy cattle farms in Egypt. The current study aimed to investigate the prevalence and major bacterial pathogens causing subclinical mastitis (SCM) in three bovine dairy herds, with a history of SCM, at three Governorates in North Upper Egypt. The antimicrobial resistance profiles and specific virulence-associated genes causing bovine SCM were investigated. One thousand sixty-quarter milk samples (QMS) were collected aseptically from 270 apparently healthy cows in three farms and examined. The total prevalence of SCM was 46% and 44.8% based on California Mastitis Test (CMT) and Somatic Cell Count (SCC), respectively. Bacteriological examination of CMT positive quarters revealed that the prevalence of bacterial isolation in subclinically mastitic quarters was 90.4% (26 and 64.3% had single and mixed isolates, respectively). The most frequent bacterial isolates were <i>E. coli</i> (49.8%), <i>Staphylococcus aureus</i> (44.9%), streptococci (44.1%) and non-<i>aureus</i> staphylococci (NAS) (37.1%). Antimicrobial susceptibility testing of isolates revealed a high degree of resistance to the most commonly used antimicrobial compound in human and veterinary medicine. Implementation of PCR revealed the presence of <i>mec</i>A and <i>bla</i>Z genes in 60% and 46.7% of <i>S. aureus</i> isolates and in 26.7% and 53.3% of NAS, respectively. Meanwhile 73.3% of streptococci isolates harbored <i>aph</i>(3’)-IIIa gene conferring resistance to aminoglycosides and <i>cfb</i> gene. All <i>E. coli</i> isolates harbored <i>tet</i>A gene conferring resistance to tetracycline and <i>sul</i>1 gene conferring resistance to sulfonamides. The <i>fim</i>H and <i>tsh</i> genes were found in 80% and 60%, respectively. A significant association between the phenotypes and genotypes of AMR in different bacteria was recorded. The presence of a high prevalence of SCM in dairy animals impacts milk production and milk quality. The coexistence of pathogenic bacteria in milk is alarming, threatens human health and has a public health significance. Herd health improvement interventions are required to protect human health and society.https://www.mdpi.com/2076-2607/9/6/1175subclinical mastitisantimicrobial resistance<i>E. coli</i><i>Staphylococcus aureus</i>NASStreptococci |
| spellingShingle | Ahmed H. Abed Ahmed M. S. Menshawy Mohamed M. A. Zeinhom Delower Hossain Eman Khalifa Gamal Wareth Mohamed F. Awad Subclinical Mastitis in Selected Bovine Dairy Herds in North Upper Egypt: Assessment of Prevalence, Causative Bacterial Pathogens, Antimicrobial Resistance and Virulence-Associated Genes Microorganisms subclinical mastitis antimicrobial resistance <i>E. coli</i> <i>Staphylococcus aureus</i> NAS Streptococci |
| title | Subclinical Mastitis in Selected Bovine Dairy Herds in North Upper Egypt: Assessment of Prevalence, Causative Bacterial Pathogens, Antimicrobial Resistance and Virulence-Associated Genes |
| title_full | Subclinical Mastitis in Selected Bovine Dairy Herds in North Upper Egypt: Assessment of Prevalence, Causative Bacterial Pathogens, Antimicrobial Resistance and Virulence-Associated Genes |
| title_fullStr | Subclinical Mastitis in Selected Bovine Dairy Herds in North Upper Egypt: Assessment of Prevalence, Causative Bacterial Pathogens, Antimicrobial Resistance and Virulence-Associated Genes |
| title_full_unstemmed | Subclinical Mastitis in Selected Bovine Dairy Herds in North Upper Egypt: Assessment of Prevalence, Causative Bacterial Pathogens, Antimicrobial Resistance and Virulence-Associated Genes |
| title_short | Subclinical Mastitis in Selected Bovine Dairy Herds in North Upper Egypt: Assessment of Prevalence, Causative Bacterial Pathogens, Antimicrobial Resistance and Virulence-Associated Genes |
| title_sort | subclinical mastitis in selected bovine dairy herds in north upper egypt assessment of prevalence causative bacterial pathogens antimicrobial resistance and virulence associated genes |
| topic | subclinical mastitis antimicrobial resistance <i>E. coli</i> <i>Staphylococcus aureus</i> NAS Streptococci |
| url | https://www.mdpi.com/2076-2607/9/6/1175 |
| work_keys_str_mv | AT ahmedhabed subclinicalmastitisinselectedbovinedairyherdsinnorthupperegyptassessmentofprevalencecausativebacterialpathogensantimicrobialresistanceandvirulenceassociatedgenes AT ahmedmsmenshawy subclinicalmastitisinselectedbovinedairyherdsinnorthupperegyptassessmentofprevalencecausativebacterialpathogensantimicrobialresistanceandvirulenceassociatedgenes AT mohamedmazeinhom subclinicalmastitisinselectedbovinedairyherdsinnorthupperegyptassessmentofprevalencecausativebacterialpathogensantimicrobialresistanceandvirulenceassociatedgenes AT delowerhossain subclinicalmastitisinselectedbovinedairyherdsinnorthupperegyptassessmentofprevalencecausativebacterialpathogensantimicrobialresistanceandvirulenceassociatedgenes AT emankhalifa subclinicalmastitisinselectedbovinedairyherdsinnorthupperegyptassessmentofprevalencecausativebacterialpathogensantimicrobialresistanceandvirulenceassociatedgenes AT gamalwareth subclinicalmastitisinselectedbovinedairyherdsinnorthupperegyptassessmentofprevalencecausativebacterialpathogensantimicrobialresistanceandvirulenceassociatedgenes AT mohamedfawad subclinicalmastitisinselectedbovinedairyherdsinnorthupperegyptassessmentofprevalencecausativebacterialpathogensantimicrobialresistanceandvirulenceassociatedgenes |