Genome-Wide Association Analyses Point to Candidate Genes for Electric Shock Avoidance in Drosophila melanogaster.
Electric shock is a common stimulus for nociception-research and the most widely used reinforcement in aversive associative learning experiments. Yet, nothing is known about the mechanisms it recruits at the periphery. To help fill this gap, we undertook a genome-wide association analysis using 38 i...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4436303?pdf=render |
| _version_ | 1819168862430560256 |
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| author | Mirjam Appel Claus-Jürgen Scholz Tobias Müller Marcus Dittrich Christian König Marie Bockstaller Tuba Oguz Afshin Khalili Emmanuel Antwi-Adjei Tamas Schauer Carla Margulies Hiromu Tanimoto Ayse Yarali |
| author_facet | Mirjam Appel Claus-Jürgen Scholz Tobias Müller Marcus Dittrich Christian König Marie Bockstaller Tuba Oguz Afshin Khalili Emmanuel Antwi-Adjei Tamas Schauer Carla Margulies Hiromu Tanimoto Ayse Yarali |
| author_sort | Mirjam Appel |
| collection | DOAJ |
| description | Electric shock is a common stimulus for nociception-research and the most widely used reinforcement in aversive associative learning experiments. Yet, nothing is known about the mechanisms it recruits at the periphery. To help fill this gap, we undertook a genome-wide association analysis using 38 inbred Drosophila melanogaster strains, which avoided shock to varying extents. We identified 514 genes whose expression levels and/ or sequences co-varied with shock avoidance scores. We independently scrutinized 14 of these genes using mutants, validating the effect of 7 of them on shock avoidance. This emphasizes the value of our candidate gene list as a guide for follow-up research. In addition, by integrating our association results with external protein-protein interaction data we obtained a shock avoidance-associated network of 38 genes. Both this network and the original candidate list contained a substantial number of genes that affect mechanosensory bristles, which are hair-like organs distributed across the fly's body. These results may point to a potential role for mechanosensory bristles in shock sensation. Thus, we not only provide a first list of candidate genes for shock avoidance, but also point to an interesting new hypothesis on nociceptive mechanisms. |
| first_indexed | 2024-12-22T19:10:21Z |
| format | Article |
| id | doaj.art-9b64e2539ad9408db662952afaf4bf14 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T19:10:21Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9b64e2539ad9408db662952afaf4bf142022-12-21T18:15:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012698610.1371/journal.pone.0126986Genome-Wide Association Analyses Point to Candidate Genes for Electric Shock Avoidance in Drosophila melanogaster.Mirjam AppelClaus-Jürgen ScholzTobias MüllerMarcus DittrichChristian KönigMarie BockstallerTuba OguzAfshin KhaliliEmmanuel Antwi-AdjeiTamas SchauerCarla MarguliesHiromu TanimotoAyse YaraliElectric shock is a common stimulus for nociception-research and the most widely used reinforcement in aversive associative learning experiments. Yet, nothing is known about the mechanisms it recruits at the periphery. To help fill this gap, we undertook a genome-wide association analysis using 38 inbred Drosophila melanogaster strains, which avoided shock to varying extents. We identified 514 genes whose expression levels and/ or sequences co-varied with shock avoidance scores. We independently scrutinized 14 of these genes using mutants, validating the effect of 7 of them on shock avoidance. This emphasizes the value of our candidate gene list as a guide for follow-up research. In addition, by integrating our association results with external protein-protein interaction data we obtained a shock avoidance-associated network of 38 genes. Both this network and the original candidate list contained a substantial number of genes that affect mechanosensory bristles, which are hair-like organs distributed across the fly's body. These results may point to a potential role for mechanosensory bristles in shock sensation. Thus, we not only provide a first list of candidate genes for shock avoidance, but also point to an interesting new hypothesis on nociceptive mechanisms.http://europepmc.org/articles/PMC4436303?pdf=render |
| spellingShingle | Mirjam Appel Claus-Jürgen Scholz Tobias Müller Marcus Dittrich Christian König Marie Bockstaller Tuba Oguz Afshin Khalili Emmanuel Antwi-Adjei Tamas Schauer Carla Margulies Hiromu Tanimoto Ayse Yarali Genome-Wide Association Analyses Point to Candidate Genes for Electric Shock Avoidance in Drosophila melanogaster. PLoS ONE |
| title | Genome-Wide Association Analyses Point to Candidate Genes for Electric Shock Avoidance in Drosophila melanogaster. |
| title_full | Genome-Wide Association Analyses Point to Candidate Genes for Electric Shock Avoidance in Drosophila melanogaster. |
| title_fullStr | Genome-Wide Association Analyses Point to Candidate Genes for Electric Shock Avoidance in Drosophila melanogaster. |
| title_full_unstemmed | Genome-Wide Association Analyses Point to Candidate Genes for Electric Shock Avoidance in Drosophila melanogaster. |
| title_short | Genome-Wide Association Analyses Point to Candidate Genes for Electric Shock Avoidance in Drosophila melanogaster. |
| title_sort | genome wide association analyses point to candidate genes for electric shock avoidance in drosophila melanogaster |
| url | http://europepmc.org/articles/PMC4436303?pdf=render |
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