Possible roles of mmu-miR-141 in the endometrium of mice in early pregnancy following embryo implantation.
OBJECTIVE: Embryo implantation is directly affected by genes related to uterine receptivity. Studies have demonstrated the important roles of miRNAs in the regulation of gene expression. Our early miRNA chip analyses revealed that the mmu-miR-141 expression in endometrial tissue is lower after embry...
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3692437?pdf=render |
| _version_ | 1819100470632775680 |
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| author | Xueqing Liu Rufei Gao Xuemei Chen Hailing Zhang Anshun Zheng Dehui Yang Yubin Ding Yingxiong Wang Junlin He |
| author_facet | Xueqing Liu Rufei Gao Xuemei Chen Hailing Zhang Anshun Zheng Dehui Yang Yubin Ding Yingxiong Wang Junlin He |
| author_sort | Xueqing Liu |
| collection | DOAJ |
| description | OBJECTIVE: Embryo implantation is directly affected by genes related to uterine receptivity. Studies have demonstrated the important roles of miRNAs in the regulation of gene expression. Our early miRNA chip analyses revealed that the mmu-miR-141 expression in endometrial tissue is lower after embryo implantation than before it. However, the possible roles of miR-141 in embryo implantation have not yet been elucidated. Here, mmu-miR-141 was designed to detect the expression and role of miR-141 in the endometria of mice in early pregnancy following embryo implantation. METHODS: Real-time PCR and in-situ hybridization were used to study mmu-miR-141 expression in mouse uterus. Cell proliferation was detected by tetrazolium dye (MTT) assay and flow cytometry. Real-time PCR and Western blot analysis were used to confirm the mRNA and protein levels of phosphatase and tensin homolog (PTEN) to determine whether it was the target gene of mmu-miR-141. Enhanced green fluorescent protein (EGFP) fluorescence reporter vector analysis was also performed. A functional study was performed by injecting mice uteri with mmu-miR-141 inhibitor or mimic vectors. RESULTS: mmu-miR-141 expression was lower on day 6 (D6) than day 4 (D4) and could be increased by progesterone. Reduced mmu-miR-141 could decrease the proliferation activity of stromal cells and promote apoptosis. Upregulation of mmu-miR-141 inhibited PTEN protein expression but downregulation of mmu-miR-141 increased it, while the mRNA level remained unchanged. EGFP fluorescence reporter vector analysis showed that miR-141 targets the 3'-untranslated region of the PTEN mRNA. In addition, when the physiological mmu-miR-141 level was altered on D2 by injecting with inhibitor or mimic, the embryo implantation sites were significantly decreased on D7. CONCLUSIONS: This study demonstrated that mmu-miR-141 might influence cell proliferation and apoptosis in the endometrium by negatively regulating PTEN expression, and could also influence the number of embryo implantation sites. mmu-miR-141 plays an essential role in embryo implantation. |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T01:03:17Z |
| publishDate | 2013-01-01 |
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| spelling | doaj.art-9b66afa8fc2f4b32b9507a2efbf293f32022-12-21T18:44:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6738210.1371/journal.pone.0067382Possible roles of mmu-miR-141 in the endometrium of mice in early pregnancy following embryo implantation.Xueqing LiuRufei GaoXuemei ChenHailing ZhangAnshun ZhengDehui YangYubin DingYingxiong WangJunlin HeOBJECTIVE: Embryo implantation is directly affected by genes related to uterine receptivity. Studies have demonstrated the important roles of miRNAs in the regulation of gene expression. Our early miRNA chip analyses revealed that the mmu-miR-141 expression in endometrial tissue is lower after embryo implantation than before it. However, the possible roles of miR-141 in embryo implantation have not yet been elucidated. Here, mmu-miR-141 was designed to detect the expression and role of miR-141 in the endometria of mice in early pregnancy following embryo implantation. METHODS: Real-time PCR and in-situ hybridization were used to study mmu-miR-141 expression in mouse uterus. Cell proliferation was detected by tetrazolium dye (MTT) assay and flow cytometry. Real-time PCR and Western blot analysis were used to confirm the mRNA and protein levels of phosphatase and tensin homolog (PTEN) to determine whether it was the target gene of mmu-miR-141. Enhanced green fluorescent protein (EGFP) fluorescence reporter vector analysis was also performed. A functional study was performed by injecting mice uteri with mmu-miR-141 inhibitor or mimic vectors. RESULTS: mmu-miR-141 expression was lower on day 6 (D6) than day 4 (D4) and could be increased by progesterone. Reduced mmu-miR-141 could decrease the proliferation activity of stromal cells and promote apoptosis. Upregulation of mmu-miR-141 inhibited PTEN protein expression but downregulation of mmu-miR-141 increased it, while the mRNA level remained unchanged. EGFP fluorescence reporter vector analysis showed that miR-141 targets the 3'-untranslated region of the PTEN mRNA. In addition, when the physiological mmu-miR-141 level was altered on D2 by injecting with inhibitor or mimic, the embryo implantation sites were significantly decreased on D7. CONCLUSIONS: This study demonstrated that mmu-miR-141 might influence cell proliferation and apoptosis in the endometrium by negatively regulating PTEN expression, and could also influence the number of embryo implantation sites. mmu-miR-141 plays an essential role in embryo implantation.http://europepmc.org/articles/PMC3692437?pdf=render |
| spellingShingle | Xueqing Liu Rufei Gao Xuemei Chen Hailing Zhang Anshun Zheng Dehui Yang Yubin Ding Yingxiong Wang Junlin He Possible roles of mmu-miR-141 in the endometrium of mice in early pregnancy following embryo implantation. PLoS ONE |
| title | Possible roles of mmu-miR-141 in the endometrium of mice in early pregnancy following embryo implantation. |
| title_full | Possible roles of mmu-miR-141 in the endometrium of mice in early pregnancy following embryo implantation. |
| title_fullStr | Possible roles of mmu-miR-141 in the endometrium of mice in early pregnancy following embryo implantation. |
| title_full_unstemmed | Possible roles of mmu-miR-141 in the endometrium of mice in early pregnancy following embryo implantation. |
| title_short | Possible roles of mmu-miR-141 in the endometrium of mice in early pregnancy following embryo implantation. |
| title_sort | possible roles of mmu mir 141 in the endometrium of mice in early pregnancy following embryo implantation |
| url | http://europepmc.org/articles/PMC3692437?pdf=render |
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