Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation
Abstract Gold standard quantitative nucleic acid tests for diagnosis of viral diseases are currently limited to implementation in laboratories outside of the clinic. An instrument for conducting nucleic acid testing at the point-of-care (POC) that is easily operable by the clinician would reduce the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2018-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-28124-3 |
| _version_ | 1818431470949105664 |
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| author | Dong Jin Shin Alexander Y. Trick Yu-Hsiang Hsieh David L. Thomas Tza-Huei Wang |
| author_facet | Dong Jin Shin Alexander Y. Trick Yu-Hsiang Hsieh David L. Thomas Tza-Huei Wang |
| author_sort | Dong Jin Shin |
| collection | DOAJ |
| description | Abstract Gold standard quantitative nucleic acid tests for diagnosis of viral diseases are currently limited to implementation in laboratories outside of the clinic. An instrument for conducting nucleic acid testing at the point-of-care (POC) that is easily operable by the clinician would reduce the required number of visits to the clinic and improve patient retention for proper treatment. Here we present a droplet magnetofluidic (DM) platform, which leverages functionalized magnetic particles to miniaturize and automate laboratory assays for use in the clinic at the POC. Our novel thermoformed disposable cartridge coupled to a portable multiaxial magnetofluidic instrument enables real-time PCR assays for quantitative and sensitive detection of nucleic acids from crude biosamples. Instead of laborious benchtop sample purification techniques followed by elution and spiking into PCR buffer, the user simply injects the biosample of interest into a cartridge with magnetic particles and loads the cartridge into the instrument. We demonstrate the utility of our platform with hepatitis C virus (HCV) RNA viral load quantitation from blood serum in approximately 1 hour. Clinical serum samples (n = 18) were directly processed on cartridges with no false positives and a limit of detection of 45 IU per 10 µl sample injection. |
| first_indexed | 2024-12-14T15:49:49Z |
| format | Article |
| id | doaj.art-9b6731c948e74900bd28c83d4ac17a05 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T15:49:49Z |
| publishDate | 2018-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9b6731c948e74900bd28c83d4ac17a052022-12-21T22:55:24ZengNature PortfolioScientific Reports2045-23222018-06-018111210.1038/s41598-018-28124-3Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load QuantitationDong Jin Shin0Alexander Y. Trick1Yu-Hsiang Hsieh2David L. Thomas3Tza-Huei Wang4Department of Mechanical Engineering, Whiting School of Engineering, The Johns Hopkins UniversityDepartment of Biomedical Engineering, Whiting School of Engineering, The Johns Hopkins UniversityDepartment of Emergency Medicine, School of Medicine, The Johns Hopkins UniversityDepartment of Medicine, School of Medicine, The Johns Hopkins UniversityDepartment of Mechanical Engineering, Whiting School of Engineering, The Johns Hopkins UniversityAbstract Gold standard quantitative nucleic acid tests for diagnosis of viral diseases are currently limited to implementation in laboratories outside of the clinic. An instrument for conducting nucleic acid testing at the point-of-care (POC) that is easily operable by the clinician would reduce the required number of visits to the clinic and improve patient retention for proper treatment. Here we present a droplet magnetofluidic (DM) platform, which leverages functionalized magnetic particles to miniaturize and automate laboratory assays for use in the clinic at the POC. Our novel thermoformed disposable cartridge coupled to a portable multiaxial magnetofluidic instrument enables real-time PCR assays for quantitative and sensitive detection of nucleic acids from crude biosamples. Instead of laborious benchtop sample purification techniques followed by elution and spiking into PCR buffer, the user simply injects the biosample of interest into a cartridge with magnetic particles and loads the cartridge into the instrument. We demonstrate the utility of our platform with hepatitis C virus (HCV) RNA viral load quantitation from blood serum in approximately 1 hour. Clinical serum samples (n = 18) were directly processed on cartridges with no false positives and a limit of detection of 45 IU per 10 µl sample injection.https://doi.org/10.1038/s41598-018-28124-3 |
| spellingShingle | Dong Jin Shin Alexander Y. Trick Yu-Hsiang Hsieh David L. Thomas Tza-Huei Wang Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation Scientific Reports |
| title | Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation |
| title_full | Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation |
| title_fullStr | Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation |
| title_full_unstemmed | Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation |
| title_short | Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation |
| title_sort | sample to answer droplet magnetofluidic platform for point of care hepatitis c viral load quantitation |
| url | https://doi.org/10.1038/s41598-018-28124-3 |
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