Thioredoxin Domain Containing 5 Suppression Elicits Serum Amyloid A-Containing High-Density Lipoproteins
Thioredoxin domain containing 5 (TXNDC5) is a protein disulfide isomerase involved in several diseases related to oxidative stress, energy metabolism and cellular inflammation. In a previous manuscript, a negative association between fatty liver development and hepatic <i>Txndc5</i> expr...
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MDPI AG
2022-03-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/3/709 |
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| author | Javier Sánchez-Marco Roberto Martínez-Beamonte Alicia De Diego Tania Herrero-Continente Cristina Barranquero Carmen Arnal Joaquín Surra María A. Navarro Jesús Osada |
| author_facet | Javier Sánchez-Marco Roberto Martínez-Beamonte Alicia De Diego Tania Herrero-Continente Cristina Barranquero Carmen Arnal Joaquín Surra María A. Navarro Jesús Osada |
| author_sort | Javier Sánchez-Marco |
| collection | DOAJ |
| description | Thioredoxin domain containing 5 (TXNDC5) is a protein disulfide isomerase involved in several diseases related to oxidative stress, energy metabolism and cellular inflammation. In a previous manuscript, a negative association between fatty liver development and hepatic <i>Txndc5</i> expression was observed. To study the role of TXNDC5 in the liver, we generated <i>Txndc5</i>-deficient mice. The absence of the protein caused an increased metabolic need to gain weight along with a bigger and fatter liver. RNAseq was performed to elucidate the putative mechanisms, showing a substantial liver overexpression of serum amyloid genes (<i>Saa1</i>, <i>Saa2</i>) with no changes in hepatic protein, but discrete plasma augmentation by the gene inactivation. Higher levels of malonyldialdehyde, apolipoprotein A1 and platelet activating factor-aryl esterase activity were also found in serum from <i>Txndc5</i>-deficient mice. However, no difference in the distribution of high-density lipoproteins (HDL)-mayor components and SAA was found between groups, and even the reactive oxygen species decreased in HDL coming from <i>Txndc5</i>-deficient mice. These results confirm the relation of this gene with hepatic steatosis and with a fasting metabolic derive remedying an acute phase response. Likewise, they pose a new role in modulating the nature of HDL particles, and SAA-containing HDL particles are not particularly oxidized. |
| first_indexed | 2024-03-09T13:49:19Z |
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| id | doaj.art-9b6b829917614855befc9c931295db84 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T13:49:19Z |
| publishDate | 2022-03-01 |
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| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-9b6b829917614855befc9c931295db842023-11-30T20:53:29ZengMDPI AGBiomedicines2227-90592022-03-0110370910.3390/biomedicines10030709Thioredoxin Domain Containing 5 Suppression Elicits Serum Amyloid A-Containing High-Density LipoproteinsJavier Sánchez-Marco0Roberto Martínez-Beamonte1Alicia De Diego2Tania Herrero-Continente3Cristina Barranquero4Carmen Arnal5Joaquín Surra6María A. Navarro7Jesús Osada8Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de Zaragoza, E-50013 Zaragoza, SpainDepartamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de Zaragoza, E-50013 Zaragoza, SpainInstituto de Investigación Sanitaria de Aragón (IISA), Universidad de Zaragoza, E-50013 Zaragoza, SpainDepartamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de Zaragoza, E-50013 Zaragoza, SpainInstituto Agroalimentario de Aragón, CITA-Universidad de Zaragoza, E-50013 Zaragoza, SpainInstituto Agroalimentario de Aragón, CITA-Universidad de Zaragoza, E-50013 Zaragoza, SpainInstituto Agroalimentario de Aragón, CITA-Universidad de Zaragoza, E-50013 Zaragoza, SpainDepartamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de Zaragoza, E-50013 Zaragoza, SpainDepartamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de Zaragoza, E-50013 Zaragoza, SpainThioredoxin domain containing 5 (TXNDC5) is a protein disulfide isomerase involved in several diseases related to oxidative stress, energy metabolism and cellular inflammation. In a previous manuscript, a negative association between fatty liver development and hepatic <i>Txndc5</i> expression was observed. To study the role of TXNDC5 in the liver, we generated <i>Txndc5</i>-deficient mice. The absence of the protein caused an increased metabolic need to gain weight along with a bigger and fatter liver. RNAseq was performed to elucidate the putative mechanisms, showing a substantial liver overexpression of serum amyloid genes (<i>Saa1</i>, <i>Saa2</i>) with no changes in hepatic protein, but discrete plasma augmentation by the gene inactivation. Higher levels of malonyldialdehyde, apolipoprotein A1 and platelet activating factor-aryl esterase activity were also found in serum from <i>Txndc5</i>-deficient mice. However, no difference in the distribution of high-density lipoproteins (HDL)-mayor components and SAA was found between groups, and even the reactive oxygen species decreased in HDL coming from <i>Txndc5</i>-deficient mice. These results confirm the relation of this gene with hepatic steatosis and with a fasting metabolic derive remedying an acute phase response. Likewise, they pose a new role in modulating the nature of HDL particles, and SAA-containing HDL particles are not particularly oxidized.https://www.mdpi.com/2227-9059/10/3/709thioredoxin domain containing 5TXNDC5serum amyloidHDLSAA<i>Saa1</i> |
| spellingShingle | Javier Sánchez-Marco Roberto Martínez-Beamonte Alicia De Diego Tania Herrero-Continente Cristina Barranquero Carmen Arnal Joaquín Surra María A. Navarro Jesús Osada Thioredoxin Domain Containing 5 Suppression Elicits Serum Amyloid A-Containing High-Density Lipoproteins Biomedicines thioredoxin domain containing 5 TXNDC5 serum amyloid HDL SAA <i>Saa1</i> |
| title | Thioredoxin Domain Containing 5 Suppression Elicits Serum Amyloid A-Containing High-Density Lipoproteins |
| title_full | Thioredoxin Domain Containing 5 Suppression Elicits Serum Amyloid A-Containing High-Density Lipoproteins |
| title_fullStr | Thioredoxin Domain Containing 5 Suppression Elicits Serum Amyloid A-Containing High-Density Lipoproteins |
| title_full_unstemmed | Thioredoxin Domain Containing 5 Suppression Elicits Serum Amyloid A-Containing High-Density Lipoproteins |
| title_short | Thioredoxin Domain Containing 5 Suppression Elicits Serum Amyloid A-Containing High-Density Lipoproteins |
| title_sort | thioredoxin domain containing 5 suppression elicits serum amyloid a containing high density lipoproteins |
| topic | thioredoxin domain containing 5 TXNDC5 serum amyloid HDL SAA <i>Saa1</i> |
| url | https://www.mdpi.com/2227-9059/10/3/709 |
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