The Possible Protective Effect of Simultaneous Administration of Alcoholic Ammi majus Seeds' Extract Against Gentamicininduced Nephrotoxicity in Rats
In gentamicin-induced nephrotoxicity, reactive oxygen species (ROS) is believed to play a pivotal role in cellular damage and necrosis via several complex mechanisms. Ammi majus L. (Apiaceae) is a rich source of many compounds which possess, among others, anti-oxidant activities. The aim of this st...
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| Format: | Article |
| Language: | English |
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College of Pharmacy / Mustansiriyah University
2012-06-01
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| Series: | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
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| Online Access: | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/240 |
| _version_ | 1797223874702606336 |
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| author | Shihab H. Muttlag Ali A. Kasim Ali R. Jassim |
| author_facet | Shihab H. Muttlag Ali A. Kasim Ali R. Jassim |
| author_sort | Shihab H. Muttlag |
| collection | DOAJ |
| description |
In gentamicin-induced nephrotoxicity, reactive oxygen species (ROS) is believed to play a pivotal role in cellular damage and necrosis via several complex mechanisms. Ammi majus L. (Apiaceae) is a rich source of many compounds which possess, among others, anti-oxidant activities.
The aim of this study is to assess the possible protective effect of the alcoholic extract of Ammi majus seeds against gentamicin-induced nephrotoxicity.
Eighteen rats were divided randomly into three groups: Group 1 (control) received a daily intraperitoneal (ip.) injection of 0.5 ml isotonic saline for six successive days. Group 2 received gentamicin dose of 100 mg kg−1day-1 (ip.), for six successive days. Group 3 received simultaneously the gentamicin dose
(ip.) and aqueous solution of Ammi majusseeds' alcoholic extract (orally), at a dose of 128 mg kg-1 day-1 for six successive days.
Serum urea, creatinin, tissue MDA and GSH concentrations were measured to assess the possible protective effects of Ammi majus.
Serum creatinine and urea levels in Ammi majus treated group were lower than the group treated with gentamicin alone (P < 0.05). Simultaneous treatment
with Ammi majus normalized the kidney tissue MDA content (P < 0.001) when compared to gentamicin-treated group. However, simultaneous treatment with
Ammi majus provided a significant increase in kidney GSH levels (P < 0.05). In conclusion Ammi majus seeds' alcoholic extract has a protective effect against gentamicin-induced nephrotoxicity when given simultaneously. This protective effect may be mediated by the antioxidant activity of its active constituents.
Further study is needed to assess the effect of treatment with Ammi majus extract before the gentamicin-induced nephrotoxicity
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| issn | 1815-0993 2959-183X |
| language | English |
| last_indexed | 2024-04-24T13:44:10Z |
| publishDate | 2012-06-01 |
| publisher | College of Pharmacy / Mustansiriyah University |
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| series | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
| spelling | doaj.art-9b6ba337428c4c99bfb774e49e1249e72024-04-04T06:58:51ZengCollege of Pharmacy / Mustansiriyah UniversityAl-Mustansiriyah Journal of Pharmaceutical Sciences1815-09932959-183X2012-06-0111110.32947/ajps.v11i1.240The Possible Protective Effect of Simultaneous Administration of Alcoholic Ammi majus Seeds' Extract Against Gentamicininduced Nephrotoxicity in RatsShihab H. Muttlag0Ali A. Kasim1Ali R. Jassim2Department of Pharmacology& ToxicologyClinical Laboratory SciencesPharmacognosy&Medicinal Plants, Collage of pharmacy, University of Baghdad, Baghdad, Iraq In gentamicin-induced nephrotoxicity, reactive oxygen species (ROS) is believed to play a pivotal role in cellular damage and necrosis via several complex mechanisms. Ammi majus L. (Apiaceae) is a rich source of many compounds which possess, among others, anti-oxidant activities. The aim of this study is to assess the possible protective effect of the alcoholic extract of Ammi majus seeds against gentamicin-induced nephrotoxicity. Eighteen rats were divided randomly into three groups: Group 1 (control) received a daily intraperitoneal (ip.) injection of 0.5 ml isotonic saline for six successive days. Group 2 received gentamicin dose of 100 mg kg−1day-1 (ip.), for six successive days. Group 3 received simultaneously the gentamicin dose (ip.) and aqueous solution of Ammi majusseeds' alcoholic extract (orally), at a dose of 128 mg kg-1 day-1 for six successive days. Serum urea, creatinin, tissue MDA and GSH concentrations were measured to assess the possible protective effects of Ammi majus. Serum creatinine and urea levels in Ammi majus treated group were lower than the group treated with gentamicin alone (P < 0.05). Simultaneous treatment with Ammi majus normalized the kidney tissue MDA content (P < 0.001) when compared to gentamicin-treated group. However, simultaneous treatment with Ammi majus provided a significant increase in kidney GSH levels (P < 0.05). In conclusion Ammi majus seeds' alcoholic extract has a protective effect against gentamicin-induced nephrotoxicity when given simultaneously. This protective effect may be mediated by the antioxidant activity of its active constituents. Further study is needed to assess the effect of treatment with Ammi majus extract before the gentamicin-induced nephrotoxicity https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/240Ammi majus, Gentamicin, Nephrotoxicity, GSH, MDA. |
| spellingShingle | Shihab H. Muttlag Ali A. Kasim Ali R. Jassim The Possible Protective Effect of Simultaneous Administration of Alcoholic Ammi majus Seeds' Extract Against Gentamicininduced Nephrotoxicity in Rats Al-Mustansiriyah Journal of Pharmaceutical Sciences Ammi majus, Gentamicin, Nephrotoxicity, GSH, MDA. |
| title | The Possible Protective Effect of Simultaneous Administration of Alcoholic Ammi majus Seeds' Extract Against Gentamicininduced Nephrotoxicity in Rats |
| title_full | The Possible Protective Effect of Simultaneous Administration of Alcoholic Ammi majus Seeds' Extract Against Gentamicininduced Nephrotoxicity in Rats |
| title_fullStr | The Possible Protective Effect of Simultaneous Administration of Alcoholic Ammi majus Seeds' Extract Against Gentamicininduced Nephrotoxicity in Rats |
| title_full_unstemmed | The Possible Protective Effect of Simultaneous Administration of Alcoholic Ammi majus Seeds' Extract Against Gentamicininduced Nephrotoxicity in Rats |
| title_short | The Possible Protective Effect of Simultaneous Administration of Alcoholic Ammi majus Seeds' Extract Against Gentamicininduced Nephrotoxicity in Rats |
| title_sort | possible protective effect of simultaneous administration of alcoholic ammi majus seeds extract against gentamicininduced nephrotoxicity in rats |
| topic | Ammi majus, Gentamicin, Nephrotoxicity, GSH, MDA. |
| url | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/240 |
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