Control of Fungal Diseases and Fruit Yield Improvement of Strawberry Using <i>Bacillus velezensis</i> CE 100
Due to the increasing health and environmental risks associated with the use of fungicides in agriculture, alternatives—such as using plant growth-promoting bacteria (PGPB) to suppress phytopathogens—that simultaneously improve plant yield, are important. This study evaluated the biocontrol efficien...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/2/365 |
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| author | Sarah Hong Tae Yoon Kim Sang-Jae Won Jae-Hyun Moon Henry B. Ajuna Kil Yong Kim Young Sang Ahn |
| author_facet | Sarah Hong Tae Yoon Kim Sang-Jae Won Jae-Hyun Moon Henry B. Ajuna Kil Yong Kim Young Sang Ahn |
| author_sort | Sarah Hong |
| collection | DOAJ |
| description | Due to the increasing health and environmental risks associated with the use of fungicides in agriculture, alternatives—such as using plant growth-promoting bacteria (PGPB) to suppress phytopathogens—that simultaneously improve plant yield, are important. This study evaluated the biocontrol efficiency of <i>Bacillus velezensis</i> CE100 against <i>Macrophomina phaseolina</i> and <i>Fusarium oxysporum f. sp. fragariae,</i> the respective causal agents for charcoal rot and fusarium wilt diseases in strawberry, and its potential to enhance strawberry growth and fruit production. <i>B. velezensis</i> CE 100 produced fungal cell wall-degrading enzymes, chitinases, and β-1,3-glucanases; and inhibited the mycelial growth of <i>M. phaseolina</i> and <i>F. oxysporum</i> f. sp. <i>fragariae</i> by 64.7% and 55.2%, respectively. The mycelia of both phytopathogenic fungi showed severe swelling and rupturing of the hyphae compared to the smooth, normal growth in the control group. Moreover, <i>B. velezensis</i> CE100 produced up to 2.8 units/mL of indole-3-acetic acid (IAA) during incubation and enhanced root biomass in strawberries. Consequently, <i>B. velezensis</i> CE 100 not only increased the fruit yield of strawberries by controlling the fungal diseases but also through enhancing plant growth. The findings of this study indicate that <i>B. velezensis</i> CE100 could be a safe, ecofriendly biocontrol alternative to chemical fungicides in strawberry production. |
| first_indexed | 2024-03-09T21:23:54Z |
| format | Article |
| id | doaj.art-9b6ef3b7d86c44b08017f1d4528c0b0d |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:23:54Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-9b6ef3b7d86c44b08017f1d4528c0b0d2023-11-23T21:14:57ZengMDPI AGMicroorganisms2076-26072022-02-0110236510.3390/microorganisms10020365Control of Fungal Diseases and Fruit Yield Improvement of Strawberry Using <i>Bacillus velezensis</i> CE 100Sarah Hong0Tae Yoon Kim1Sang-Jae Won2Jae-Hyun Moon3Henry B. Ajuna4Kil Yong Kim5Young Sang Ahn6Department of Chemical Engineering, University of California, Davis, CA 95616, USADepartment of Plant Sciences, University of California, Davis, CA 95616, USADepartment of Forest Resources, Chonnam National University, Gwangju 61186, KoreaDepartment of Forest Resources, Chonnam National University, Gwangju 61186, KoreaDepartment of Forest Resources, Chonnam National University, Gwangju 61186, KoreaDivision of Agricultural and Biological Chemistry, Institute of Environmentally Friendly Agriculture, Chonnam National University, Gwangju 61186, KoreaDepartment of Forest Resources, Chonnam National University, Gwangju 61186, KoreaDue to the increasing health and environmental risks associated with the use of fungicides in agriculture, alternatives—such as using plant growth-promoting bacteria (PGPB) to suppress phytopathogens—that simultaneously improve plant yield, are important. This study evaluated the biocontrol efficiency of <i>Bacillus velezensis</i> CE100 against <i>Macrophomina phaseolina</i> and <i>Fusarium oxysporum f. sp. fragariae,</i> the respective causal agents for charcoal rot and fusarium wilt diseases in strawberry, and its potential to enhance strawberry growth and fruit production. <i>B. velezensis</i> CE 100 produced fungal cell wall-degrading enzymes, chitinases, and β-1,3-glucanases; and inhibited the mycelial growth of <i>M. phaseolina</i> and <i>F. oxysporum</i> f. sp. <i>fragariae</i> by 64.7% and 55.2%, respectively. The mycelia of both phytopathogenic fungi showed severe swelling and rupturing of the hyphae compared to the smooth, normal growth in the control group. Moreover, <i>B. velezensis</i> CE100 produced up to 2.8 units/mL of indole-3-acetic acid (IAA) during incubation and enhanced root biomass in strawberries. Consequently, <i>B. velezensis</i> CE 100 not only increased the fruit yield of strawberries by controlling the fungal diseases but also through enhancing plant growth. The findings of this study indicate that <i>B. velezensis</i> CE100 could be a safe, ecofriendly biocontrol alternative to chemical fungicides in strawberry production.https://www.mdpi.com/2076-2607/10/2/365biocontrol agentlytic enzymesphytopathogenic fungiauxinstrawberry production |
| spellingShingle | Sarah Hong Tae Yoon Kim Sang-Jae Won Jae-Hyun Moon Henry B. Ajuna Kil Yong Kim Young Sang Ahn Control of Fungal Diseases and Fruit Yield Improvement of Strawberry Using <i>Bacillus velezensis</i> CE 100 Microorganisms biocontrol agent lytic enzymes phytopathogenic fungi auxin strawberry production |
| title | Control of Fungal Diseases and Fruit Yield Improvement of Strawberry Using <i>Bacillus velezensis</i> CE 100 |
| title_full | Control of Fungal Diseases and Fruit Yield Improvement of Strawberry Using <i>Bacillus velezensis</i> CE 100 |
| title_fullStr | Control of Fungal Diseases and Fruit Yield Improvement of Strawberry Using <i>Bacillus velezensis</i> CE 100 |
| title_full_unstemmed | Control of Fungal Diseases and Fruit Yield Improvement of Strawberry Using <i>Bacillus velezensis</i> CE 100 |
| title_short | Control of Fungal Diseases and Fruit Yield Improvement of Strawberry Using <i>Bacillus velezensis</i> CE 100 |
| title_sort | control of fungal diseases and fruit yield improvement of strawberry using i bacillus velezensis i ce 100 |
| topic | biocontrol agent lytic enzymes phytopathogenic fungi auxin strawberry production |
| url | https://www.mdpi.com/2076-2607/10/2/365 |
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