The Bacterial DNA Profiling of Chorionic Villi and Amniotic Fluids Reveals Overlaps with Maternal Oral, Vaginal, and Gut Microbiomes
The in utero microbiome hypothesis has been long debated. This hypothesis will change our comprehension of the pioneer human microbiome if proved correct. In 60 uncomplicated pregnancies, we profiled the microbiome of chorionic villi (CV) and amniotic fluids (AF) in relation to maternal saliva, rect...
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2873 |
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| author | Giuseppina Campisciano Nunzia Zanotta Mariachiara Quadrifoglio Annalisa Careri Alessandra Torresani Carolina Cason Francesco De Seta Giuseppe Ricci Manola Comar Tamara Stampalija |
| author_facet | Giuseppina Campisciano Nunzia Zanotta Mariachiara Quadrifoglio Annalisa Careri Alessandra Torresani Carolina Cason Francesco De Seta Giuseppe Ricci Manola Comar Tamara Stampalija |
| author_sort | Giuseppina Campisciano |
| collection | DOAJ |
| description | The in utero microbiome hypothesis has been long debated. This hypothesis will change our comprehension of the pioneer human microbiome if proved correct. In 60 uncomplicated pregnancies, we profiled the microbiome of chorionic villi (CV) and amniotic fluids (AF) in relation to maternal saliva, rectum, and vagina and the soluble cytokines cascade in the vagina, CV and AF. In our series, 12/37 (32%) AF and 10/23 (44%) CV tested positive for bacterial DNA. CV and AF harbored bacterial DNA of Streptococcus and Lactobacillus, overlapping that of the matched oral and vaginal niches, which showed a dysbiotic microbiome. In these pregnant women, the immune profiling revealed an immune hyporesponsiveness in the vagina and a high intraamniotic concentration of inflammatory cytokines. To understand the eventual role of bacterial colonization of the CV and AF and the associated immune response in the pregnancy outcome, further appropriate studies are needed. In this context, further studies should highlight if the hematogenous route could justify the spread of bacterial DNA from the oral microbiome to the placenta and if vaginal dysbiosis could favor the likelihood of identifying CV and AF positive for bacterial DNA. |
| first_indexed | 2024-03-11T09:40:05Z |
| format | Article |
| id | doaj.art-9b6faa6a721b40a6b55a4df77f9edcf1 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:40:05Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9b6faa6a721b40a6b55a4df77f9edcf12023-11-16T17:03:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01243287310.3390/ijms24032873The Bacterial DNA Profiling of Chorionic Villi and Amniotic Fluids Reveals Overlaps with Maternal Oral, Vaginal, and Gut MicrobiomesGiuseppina Campisciano0Nunzia Zanotta1Mariachiara Quadrifoglio2Annalisa Careri3Alessandra Torresani4Carolina Cason5Francesco De Seta6Giuseppe Ricci7Manola Comar8Tamara Stampalija9Department of Advanced Translational Microbiology, Institute for Maternal and Child Health—IRCCS Burlo Garofolo, Via dell’Istria, 65, 34137 Trieste, ItalyDepartment of Advanced Translational Microbiology, Institute for Maternal and Child Health—IRCCS Burlo Garofolo, Via dell’Istria, 65, 34137 Trieste, ItalyUnit of Fetal Medicine and Prenatal Diagnosis, Institute for Maternal and Child Health—IRCCS Burlo Garofolo, Via dell’Istria, 65, 34137 Trieste, ItalyDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Strada di Fiume 447, 34149 Trieste, ItalyDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Strada di Fiume 447, 34149 Trieste, ItalyDepartment of Advanced Translational Microbiology, Institute for Maternal and Child Health—IRCCS Burlo Garofolo, Via dell’Istria, 65, 34137 Trieste, ItalyDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Strada di Fiume 447, 34149 Trieste, ItalyDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Strada di Fiume 447, 34149 Trieste, ItalyDepartment of Advanced Translational Microbiology, Institute for Maternal and Child Health—IRCCS Burlo Garofolo, Via dell’Istria, 65, 34137 Trieste, ItalyUnit of Fetal Medicine and Prenatal Diagnosis, Institute for Maternal and Child Health—IRCCS Burlo Garofolo, Via dell’Istria, 65, 34137 Trieste, ItalyThe in utero microbiome hypothesis has been long debated. This hypothesis will change our comprehension of the pioneer human microbiome if proved correct. In 60 uncomplicated pregnancies, we profiled the microbiome of chorionic villi (CV) and amniotic fluids (AF) in relation to maternal saliva, rectum, and vagina and the soluble cytokines cascade in the vagina, CV and AF. In our series, 12/37 (32%) AF and 10/23 (44%) CV tested positive for bacterial DNA. CV and AF harbored bacterial DNA of Streptococcus and Lactobacillus, overlapping that of the matched oral and vaginal niches, which showed a dysbiotic microbiome. In these pregnant women, the immune profiling revealed an immune hyporesponsiveness in the vagina and a high intraamniotic concentration of inflammatory cytokines. To understand the eventual role of bacterial colonization of the CV and AF and the associated immune response in the pregnancy outcome, further appropriate studies are needed. In this context, further studies should highlight if the hematogenous route could justify the spread of bacterial DNA from the oral microbiome to the placenta and if vaginal dysbiosis could favor the likelihood of identifying CV and AF positive for bacterial DNA.https://www.mdpi.com/1422-0067/24/3/2873maternal-fetal microbiota axisin utero microbiomesterile womb hypothesispregnancychorionic villiamniotic fluid |
| spellingShingle | Giuseppina Campisciano Nunzia Zanotta Mariachiara Quadrifoglio Annalisa Careri Alessandra Torresani Carolina Cason Francesco De Seta Giuseppe Ricci Manola Comar Tamara Stampalija The Bacterial DNA Profiling of Chorionic Villi and Amniotic Fluids Reveals Overlaps with Maternal Oral, Vaginal, and Gut Microbiomes International Journal of Molecular Sciences maternal-fetal microbiota axis in utero microbiome sterile womb hypothesis pregnancy chorionic villi amniotic fluid |
| title | The Bacterial DNA Profiling of Chorionic Villi and Amniotic Fluids Reveals Overlaps with Maternal Oral, Vaginal, and Gut Microbiomes |
| title_full | The Bacterial DNA Profiling of Chorionic Villi and Amniotic Fluids Reveals Overlaps with Maternal Oral, Vaginal, and Gut Microbiomes |
| title_fullStr | The Bacterial DNA Profiling of Chorionic Villi and Amniotic Fluids Reveals Overlaps with Maternal Oral, Vaginal, and Gut Microbiomes |
| title_full_unstemmed | The Bacterial DNA Profiling of Chorionic Villi and Amniotic Fluids Reveals Overlaps with Maternal Oral, Vaginal, and Gut Microbiomes |
| title_short | The Bacterial DNA Profiling of Chorionic Villi and Amniotic Fluids Reveals Overlaps with Maternal Oral, Vaginal, and Gut Microbiomes |
| title_sort | bacterial dna profiling of chorionic villi and amniotic fluids reveals overlaps with maternal oral vaginal and gut microbiomes |
| topic | maternal-fetal microbiota axis in utero microbiome sterile womb hypothesis pregnancy chorionic villi amniotic fluid |
| url | https://www.mdpi.com/1422-0067/24/3/2873 |
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