In Vitro Antifungal Activity and Mechanism of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> Composites against <i>Candida</i> Species
Fungal infections pose a serious threat to human health. Polyoxometalates (POMs) are metal–oxygen clusters with potential application in the control of microbial infections. Herein, the Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> composites have been synthesized a...
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MDPI AG
2020-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/24/6012 |
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| author | Xinming Zhang Tianzhan Zhang Shuanli Guo Yang Zhang Rongtian Sheng Ruimeng Sun Lixia Chen Ruijuan Lv Yanfei Qi |
| author_facet | Xinming Zhang Tianzhan Zhang Shuanli Guo Yang Zhang Rongtian Sheng Ruimeng Sun Lixia Chen Ruijuan Lv Yanfei Qi |
| author_sort | Xinming Zhang |
| collection | DOAJ |
| description | Fungal infections pose a serious threat to human health. Polyoxometalates (POMs) are metal–oxygen clusters with potential application in the control of microbial infections. Herein, the Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> composites have been synthesized and verified by Fourier transform infrared (FT-IR) spectrum, transmission electron microscopy (TEM), scanning electron microscope (SEM), elemental analysis, and X-ray diffraction (XRD). The antifungal activities of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> were screened in 19 <i>Candida</i> species strains through the determination of minimum inhibitory concentration (MIC) by the microdilution checkerboard technique. The minimum inhibitory concentration (MIC<sub>50</sub>) values of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> are 2~32 μg/mL to the <i>Candida</i> species. The MIC<sub>80</sub> value of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> to resistant clinical isolates <i>C. albicans</i> HL963 is 8 μg/mL, which is lower than the positive control, fluconazole (FLC). The mechanism against <i>C. albicans</i> HL963 results show that Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> can decrease the ergosterol content. The expressions of ERG1, ERG7, and ERG11, which impact on the synthesis of ergosterol, are all prominently upregulated by Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub>. It indicates that Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> is a candidate in the development of new antifungal agents. |
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| institution | Directory Open Access Journal |
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| language | English |
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| spelling | doaj.art-9b6fb40665444fc3868f44adb59372942023-11-21T01:34:25ZengMDPI AGMolecules1420-30492020-12-012524601210.3390/molecules25246012In Vitro Antifungal Activity and Mechanism of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> Composites against <i>Candida</i> SpeciesXinming Zhang0Tianzhan Zhang1Shuanli Guo2Yang Zhang3Rongtian Sheng4Ruimeng Sun5Lixia Chen6Ruijuan Lv7Yanfei Qi8School of Public Health, Jilin University, Changchun 130021, ChinaCollege of Material Science and Engineering, Jilin Jianzhu University, Changchun 130021, ChinaSchool of Public Health, Jilin University, Changchun 130021, ChinaSchool of Public Health, Jilin University, Changchun 130021, ChinaSchool of Public Health, Jilin University, Changchun 130021, ChinaSchool of Public Health, Jilin University, Changchun 130021, ChinaSchool of Public Health, Jilin University, Changchun 130021, ChinaSchool of Public Health, Jilin University, Changchun 130021, ChinaSchool of Public Health, Jilin University, Changchun 130021, ChinaFungal infections pose a serious threat to human health. Polyoxometalates (POMs) are metal–oxygen clusters with potential application in the control of microbial infections. Herein, the Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> composites have been synthesized and verified by Fourier transform infrared (FT-IR) spectrum, transmission electron microscopy (TEM), scanning electron microscope (SEM), elemental analysis, and X-ray diffraction (XRD). The antifungal activities of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> were screened in 19 <i>Candida</i> species strains through the determination of minimum inhibitory concentration (MIC) by the microdilution checkerboard technique. The minimum inhibitory concentration (MIC<sub>50</sub>) values of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> are 2~32 μg/mL to the <i>Candida</i> species. The MIC<sub>80</sub> value of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> to resistant clinical isolates <i>C. albicans</i> HL963 is 8 μg/mL, which is lower than the positive control, fluconazole (FLC). The mechanism against <i>C. albicans</i> HL963 results show that Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> can decrease the ergosterol content. The expressions of ERG1, ERG7, and ERG11, which impact on the synthesis of ergosterol, are all prominently upregulated by Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub>. It indicates that Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> is a candidate in the development of new antifungal agents.https://www.mdpi.com/1420-3049/25/24/6012polyoxometalatespompositesantifungal activityergosterolRT-PCR |
| spellingShingle | Xinming Zhang Tianzhan Zhang Shuanli Guo Yang Zhang Rongtian Sheng Ruimeng Sun Lixia Chen Ruijuan Lv Yanfei Qi In Vitro Antifungal Activity and Mechanism of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> Composites against <i>Candida</i> Species Molecules polyoxometalates pomposites antifungal activity ergosterol RT-PCR |
| title | In Vitro Antifungal Activity and Mechanism of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> Composites against <i>Candida</i> Species |
| title_full | In Vitro Antifungal Activity and Mechanism of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> Composites against <i>Candida</i> Species |
| title_fullStr | In Vitro Antifungal Activity and Mechanism of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> Composites against <i>Candida</i> Species |
| title_full_unstemmed | In Vitro Antifungal Activity and Mechanism of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> Composites against <i>Candida</i> Species |
| title_short | In Vitro Antifungal Activity and Mechanism of Ag<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> Composites against <i>Candida</i> Species |
| title_sort | in vitro antifungal activity and mechanism of ag sub 3 sub pw sub 12 sub o sub 40 sub composites against i candida i species |
| topic | polyoxometalates pomposites antifungal activity ergosterol RT-PCR |
| url | https://www.mdpi.com/1420-3049/25/24/6012 |
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