Genome-wide Translational Changes Induced by the Prion [PSI+]
Prions are infectious proteins that can adopt a structural conformation that is then propagated among other molecules of the same protein. [PSI+] is an aggregated conformation of the translational release factor eRF3. [PSI+] modifies cellular fitness, inducing various phenotypes depending on genetic...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-07-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124714005208 |
| _version_ | 1818849776994615296 |
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| author | Agnès Baudin-Baillieu Rachel Legendre Claire Kuchly Isabelle Hatin Stéphane Demais Claire Mestdagh Daniel Gautheret Olivier Namy |
| author_facet | Agnès Baudin-Baillieu Rachel Legendre Claire Kuchly Isabelle Hatin Stéphane Demais Claire Mestdagh Daniel Gautheret Olivier Namy |
| author_sort | Agnès Baudin-Baillieu |
| collection | DOAJ |
| description | Prions are infectious proteins that can adopt a structural conformation that is then propagated among other molecules of the same protein. [PSI+] is an aggregated conformation of the translational release factor eRF3. [PSI+] modifies cellular fitness, inducing various phenotypes depending on genetic background. However, the genes displaying [PSI+]-controlled expression remain unknown. We used ribosome profiling in isogenic [PSI+] and [psi−] strains to identify the changes induced by [PSI+]. We found 100 genes with stop codon readthrough events and showed that many stress-response genes were repressed in the presence of [PSI+]. Surprisingly, [PSI+] was also found to affect reading frame selection independently of its effect on translation termination efficiency. These results indicate that [PSI+] has a broader impact than initially anticipated, providing explanations for the phenotypic differences between [psi−] and [PSI+] strains. |
| first_indexed | 2024-12-19T06:38:37Z |
| format | Article |
| id | doaj.art-9b7bdff25c404eb881fa674d8f012b75 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-19T06:38:37Z |
| publishDate | 2014-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-9b7bdff25c404eb881fa674d8f012b752022-12-21T20:32:10ZengElsevierCell Reports2211-12472014-07-018243944810.1016/j.celrep.2014.06.036Genome-wide Translational Changes Induced by the Prion [PSI+]Agnès Baudin-Baillieu0Rachel Legendre1Claire Kuchly2Isabelle Hatin3Stéphane Demais4Claire Mestdagh5Daniel Gautheret6Olivier Namy7IGM, University of Paris-Sud, 91405 Orsay, FranceIGM, University of Paris-Sud, 91405 Orsay, FranceIGM, University of Paris-Sud, 91405 Orsay, FranceIGM, University of Paris-Sud, 91405 Orsay, FranceIGM, University of Paris-Sud, 91405 Orsay, FranceIGM, University of Paris-Sud, 91405 Orsay, FranceIGM, University of Paris-Sud, 91405 Orsay, FranceIGM, University of Paris-Sud, 91405 Orsay, FrancePrions are infectious proteins that can adopt a structural conformation that is then propagated among other molecules of the same protein. [PSI+] is an aggregated conformation of the translational release factor eRF3. [PSI+] modifies cellular fitness, inducing various phenotypes depending on genetic background. However, the genes displaying [PSI+]-controlled expression remain unknown. We used ribosome profiling in isogenic [PSI+] and [psi−] strains to identify the changes induced by [PSI+]. We found 100 genes with stop codon readthrough events and showed that many stress-response genes were repressed in the presence of [PSI+]. Surprisingly, [PSI+] was also found to affect reading frame selection independently of its effect on translation termination efficiency. These results indicate that [PSI+] has a broader impact than initially anticipated, providing explanations for the phenotypic differences between [psi−] and [PSI+] strains.http://www.sciencedirect.com/science/article/pii/S2211124714005208 |
| spellingShingle | Agnès Baudin-Baillieu Rachel Legendre Claire Kuchly Isabelle Hatin Stéphane Demais Claire Mestdagh Daniel Gautheret Olivier Namy Genome-wide Translational Changes Induced by the Prion [PSI+] Cell Reports |
| title | Genome-wide Translational Changes Induced by the Prion [PSI+] |
| title_full | Genome-wide Translational Changes Induced by the Prion [PSI+] |
| title_fullStr | Genome-wide Translational Changes Induced by the Prion [PSI+] |
| title_full_unstemmed | Genome-wide Translational Changes Induced by the Prion [PSI+] |
| title_short | Genome-wide Translational Changes Induced by the Prion [PSI+] |
| title_sort | genome wide translational changes induced by the prion psi |
| url | http://www.sciencedirect.com/science/article/pii/S2211124714005208 |
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