Dietary effects of supplementing Clostridium butyricum culture or sodium butyrate in low fishmeal diet on growth, serum indicators, intestinal histology and microbiota of largemouth bass (Micropterus salmoides)
The present study aimed to evaluate the dietary effects of Clostridium butyricum culture (CC) or sodium butyrate (SB) on the growth, feed utilization, serum indicators, intestinal histology and microbiota of largemouth bass (Micropterus salmoides). CC and coated SB (50%) were supplemented to a low f...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423003666 |
| _version_ | 1797388829333651456 |
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| author | Xia Lin Yunfeng Chen Supranee Ruenkoed Xiaoqin Li Xiangjun Leng |
| author_facet | Xia Lin Yunfeng Chen Supranee Ruenkoed Xiaoqin Li Xiangjun Leng |
| author_sort | Xia Lin |
| collection | DOAJ |
| description | The present study aimed to evaluate the dietary effects of Clostridium butyricum culture (CC) or sodium butyrate (SB) on the growth, feed utilization, serum indicators, intestinal histology and microbiota of largemouth bass (Micropterus salmoides). CC and coated SB (50%) were supplemented to a low fish meal diet (245 g/kg) (control diet) at the inclusion levels of 1 g/kg and 2 g/kg, to form five iso-nitrogenous and iso-lipidic diets (control, CC-1, CC-2, SB-1, and SB-2). Then, the five diets were fed to largemouth bass with initial body weight of 70.2 ± 0.1 g. After 8 weeks of feeding, dietary supplementation of 1 g/kg CC increased the weight gain (WG) by 7.4% (P < 0.05), and decreased the feed conversion ratio by 0.09 (P < 0.05), while dietary SB just numerically improved the WG (P > 0.05), when compared to the control. The muscle composition was not significantly affected by the inclusion of CC or SB (P > 0.05). Dietary inclusion of CC or SB significantly reduced serum diamine oxidase activity and increased lysozyme activity (P < 0.05), while the decreased D-lactic acid level was observed only in the two SB groups (P < 0.05). In intestinal histology, the 1.0 g/kg CC group presented significantly higher intestinal villus height, width and muscle layer thickness than the control group (P < 0.05). The intestinal microbiota analysis showed that dietary CC or SB (1 g/kg) increased the abundance of Fusobacteriota and Cetobacterium, decreased the abundance of Proteobacteria and Aeromonas. In conclusion, the supplementation of CC in low fish meal diet promoted the growth performance, feed utilization, and both CC and SB supplementation positively modulated the intestinal microorganism community of largemouth bass. |
| first_indexed | 2024-03-08T22:46:29Z |
| format | Article |
| id | doaj.art-9b7be28a9bb24b4a9bf1b18e6b21a87a |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-08T22:46:29Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-9b7be28a9bb24b4a9bf1b18e6b21a87a2023-12-17T06:39:52ZengElsevierAquaculture Reports2352-51342023-12-0133101827Dietary effects of supplementing Clostridium butyricum culture or sodium butyrate in low fishmeal diet on growth, serum indicators, intestinal histology and microbiota of largemouth bass (Micropterus salmoides)Xia Lin0Yunfeng Chen1Supranee Ruenkoed2Xiaoqin Li3Xiangjun Leng4National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, ChinaNational Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, ChinaResearch Institute, Pucheng Chia Tai Biochemistry Co., Ltd., Pucheng, Fujian 353400, ChinaNational Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China; Correspondence to: Hucheng Ring road 999, Lingang New City, Shanghai 201306, China.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China; Correspondence to: Hucheng Ring road 999, Lingang New City, Shanghai 201306, China.The present study aimed to evaluate the dietary effects of Clostridium butyricum culture (CC) or sodium butyrate (SB) on the growth, feed utilization, serum indicators, intestinal histology and microbiota of largemouth bass (Micropterus salmoides). CC and coated SB (50%) were supplemented to a low fish meal diet (245 g/kg) (control diet) at the inclusion levels of 1 g/kg and 2 g/kg, to form five iso-nitrogenous and iso-lipidic diets (control, CC-1, CC-2, SB-1, and SB-2). Then, the five diets were fed to largemouth bass with initial body weight of 70.2 ± 0.1 g. After 8 weeks of feeding, dietary supplementation of 1 g/kg CC increased the weight gain (WG) by 7.4% (P < 0.05), and decreased the feed conversion ratio by 0.09 (P < 0.05), while dietary SB just numerically improved the WG (P > 0.05), when compared to the control. The muscle composition was not significantly affected by the inclusion of CC or SB (P > 0.05). Dietary inclusion of CC or SB significantly reduced serum diamine oxidase activity and increased lysozyme activity (P < 0.05), while the decreased D-lactic acid level was observed only in the two SB groups (P < 0.05). In intestinal histology, the 1.0 g/kg CC group presented significantly higher intestinal villus height, width and muscle layer thickness than the control group (P < 0.05). The intestinal microbiota analysis showed that dietary CC or SB (1 g/kg) increased the abundance of Fusobacteriota and Cetobacterium, decreased the abundance of Proteobacteria and Aeromonas. In conclusion, the supplementation of CC in low fish meal diet promoted the growth performance, feed utilization, and both CC and SB supplementation positively modulated the intestinal microorganism community of largemouth bass.http://www.sciencedirect.com/science/article/pii/S2352513423003666Clostridium butyricum cultureSodium butyrateLargemouth bassGrowthSerum indicatorsIntestinal histology |
| spellingShingle | Xia Lin Yunfeng Chen Supranee Ruenkoed Xiaoqin Li Xiangjun Leng Dietary effects of supplementing Clostridium butyricum culture or sodium butyrate in low fishmeal diet on growth, serum indicators, intestinal histology and microbiota of largemouth bass (Micropterus salmoides) Aquaculture Reports Clostridium butyricum culture Sodium butyrate Largemouth bass Growth Serum indicators Intestinal histology |
| title | Dietary effects of supplementing Clostridium butyricum culture or sodium butyrate in low fishmeal diet on growth, serum indicators, intestinal histology and microbiota of largemouth bass (Micropterus salmoides) |
| title_full | Dietary effects of supplementing Clostridium butyricum culture or sodium butyrate in low fishmeal diet on growth, serum indicators, intestinal histology and microbiota of largemouth bass (Micropterus salmoides) |
| title_fullStr | Dietary effects of supplementing Clostridium butyricum culture or sodium butyrate in low fishmeal diet on growth, serum indicators, intestinal histology and microbiota of largemouth bass (Micropterus salmoides) |
| title_full_unstemmed | Dietary effects of supplementing Clostridium butyricum culture or sodium butyrate in low fishmeal diet on growth, serum indicators, intestinal histology and microbiota of largemouth bass (Micropterus salmoides) |
| title_short | Dietary effects of supplementing Clostridium butyricum culture or sodium butyrate in low fishmeal diet on growth, serum indicators, intestinal histology and microbiota of largemouth bass (Micropterus salmoides) |
| title_sort | dietary effects of supplementing clostridium butyricum culture or sodium butyrate in low fishmeal diet on growth serum indicators intestinal histology and microbiota of largemouth bass micropterus salmoides |
| topic | Clostridium butyricum culture Sodium butyrate Largemouth bass Growth Serum indicators Intestinal histology |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423003666 |
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