The Association of Rpb4 with RNA Polymerase II Depends on CTD Ser5P Phosphatase Rtr1 and Influences mRNA Decay in <i>Saccharomyces cerevisiae</i>
Rtr1 is an RNA polymerase II (RNA pol II) CTD-phosphatase that influences gene expression during the transition from transcription initiation to elongation and during transcription termination. Rtr1 interacts with the RNA pol II and this interaction depends on the phosphorylation state of the CTD of...
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MDPI AG
2022-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/4/2002 |
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| author | Ana I. Garrido-Godino Abel Cuevas-Bermúdez Francisco Gutiérrez-Santiago Maria del Carmen Mota-Trujillo Francisco Navarro |
| author_facet | Ana I. Garrido-Godino Abel Cuevas-Bermúdez Francisco Gutiérrez-Santiago Maria del Carmen Mota-Trujillo Francisco Navarro |
| author_sort | Ana I. Garrido-Godino |
| collection | DOAJ |
| description | Rtr1 is an RNA polymerase II (RNA pol II) CTD-phosphatase that influences gene expression during the transition from transcription initiation to elongation and during transcription termination. Rtr1 interacts with the RNA pol II and this interaction depends on the phosphorylation state of the CTD of Rpb1, which may influence dissociation of the heterodimer Rpb4/7 during transcription. In addition, Rtr1 was proposed as an RNA pol II import factor in RNA pol II biogenesis and participates in mRNA decay by autoregulating the turnover of its own mRNA. Our work shows that Rtr1 acts in RNA pol II assembly by mediating the Rpb4/7 association with the rest of the enzyme. <i>RTR1</i> deletion alters RNA pol II assembly and increases the amount of RNA pol II associated with the chromatin that lacks Rpb4, decreasing Rpb4-mRNA imprinting and, consequently, increasing mRNA stability. Thus, Rtr1 interplays RNA pol II biogenesis and mRNA decay regulation. Our data also indicate that Rtr1 mediates mRNA decay regulation more broadly than previously proposed by cooperating with Rpb4. Interestingly, our data include new layers in the mechanisms of gene regulation and in the crosstalk between mRNA synthesis and decay by demonstrating how the association of Rpb4/7 to the RNA pol II influences mRNA decay. |
| first_indexed | 2024-03-09T21:46:12Z |
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| id | doaj.art-9b7dd34c7f6e42a6a6e1dfadfe5de411 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:46:12Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9b7dd34c7f6e42a6a6e1dfadfe5de4112023-11-23T20:18:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01234200210.3390/ijms23042002The Association of Rpb4 with RNA Polymerase II Depends on CTD Ser5P Phosphatase Rtr1 and Influences mRNA Decay in <i>Saccharomyces cerevisiae</i>Ana I. Garrido-Godino0Abel Cuevas-Bermúdez1Francisco Gutiérrez-Santiago2Maria del Carmen Mota-Trujillo3Francisco Navarro4Departamento de Biología Experimental-Genética, Universidad de Jaén, Paraje de las Lagunillas, s/n, E-23071 Jaén, SpainDepartamento de Biología Experimental-Genética, Universidad de Jaén, Paraje de las Lagunillas, s/n, E-23071 Jaén, SpainDepartamento de Biología Experimental-Genética, Universidad de Jaén, Paraje de las Lagunillas, s/n, E-23071 Jaén, SpainDepartamento de Biología Experimental-Genética, Universidad de Jaén, Paraje de las Lagunillas, s/n, E-23071 Jaén, SpainDepartamento de Biología Experimental-Genética, Universidad de Jaén, Paraje de las Lagunillas, s/n, E-23071 Jaén, SpainRtr1 is an RNA polymerase II (RNA pol II) CTD-phosphatase that influences gene expression during the transition from transcription initiation to elongation and during transcription termination. Rtr1 interacts with the RNA pol II and this interaction depends on the phosphorylation state of the CTD of Rpb1, which may influence dissociation of the heterodimer Rpb4/7 during transcription. In addition, Rtr1 was proposed as an RNA pol II import factor in RNA pol II biogenesis and participates in mRNA decay by autoregulating the turnover of its own mRNA. Our work shows that Rtr1 acts in RNA pol II assembly by mediating the Rpb4/7 association with the rest of the enzyme. <i>RTR1</i> deletion alters RNA pol II assembly and increases the amount of RNA pol II associated with the chromatin that lacks Rpb4, decreasing Rpb4-mRNA imprinting and, consequently, increasing mRNA stability. Thus, Rtr1 interplays RNA pol II biogenesis and mRNA decay regulation. Our data also indicate that Rtr1 mediates mRNA decay regulation more broadly than previously proposed by cooperating with Rpb4. Interestingly, our data include new layers in the mechanisms of gene regulation and in the crosstalk between mRNA synthesis and decay by demonstrating how the association of Rpb4/7 to the RNA pol II influences mRNA decay.https://www.mdpi.com/1422-0067/23/4/2002transcriptionRNA polymerasesbiogenesismRNA stabilityRtr1 CTD phosphatase<i>Saccharomyces cerevisiae</i> |
| spellingShingle | Ana I. Garrido-Godino Abel Cuevas-Bermúdez Francisco Gutiérrez-Santiago Maria del Carmen Mota-Trujillo Francisco Navarro The Association of Rpb4 with RNA Polymerase II Depends on CTD Ser5P Phosphatase Rtr1 and Influences mRNA Decay in <i>Saccharomyces cerevisiae</i> International Journal of Molecular Sciences transcription RNA polymerases biogenesis mRNA stability Rtr1 CTD phosphatase <i>Saccharomyces cerevisiae</i> |
| title | The Association of Rpb4 with RNA Polymerase II Depends on CTD Ser5P Phosphatase Rtr1 and Influences mRNA Decay in <i>Saccharomyces cerevisiae</i> |
| title_full | The Association of Rpb4 with RNA Polymerase II Depends on CTD Ser5P Phosphatase Rtr1 and Influences mRNA Decay in <i>Saccharomyces cerevisiae</i> |
| title_fullStr | The Association of Rpb4 with RNA Polymerase II Depends on CTD Ser5P Phosphatase Rtr1 and Influences mRNA Decay in <i>Saccharomyces cerevisiae</i> |
| title_full_unstemmed | The Association of Rpb4 with RNA Polymerase II Depends on CTD Ser5P Phosphatase Rtr1 and Influences mRNA Decay in <i>Saccharomyces cerevisiae</i> |
| title_short | The Association of Rpb4 with RNA Polymerase II Depends on CTD Ser5P Phosphatase Rtr1 and Influences mRNA Decay in <i>Saccharomyces cerevisiae</i> |
| title_sort | association of rpb4 with rna polymerase ii depends on ctd ser5p phosphatase rtr1 and influences mrna decay in i saccharomyces cerevisiae i |
| topic | transcription RNA polymerases biogenesis mRNA stability Rtr1 CTD phosphatase <i>Saccharomyces cerevisiae</i> |
| url | https://www.mdpi.com/1422-0067/23/4/2002 |
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