Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep
Silage is rich in nutrients, which can make up for the lack of seasonal roughage, and has a certain promotion effect on the intensive feeding of ruminants. In addition, silage can maintain the rumen function of ruminants to a certain extent and reduce the risk of rumen acidosis and abomasum transloc...
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| Format: | Article |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.978940/full |
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| author | Chuang Li Chuang Li Ning Chen Ning Chen Xingxing Zhang Xingxing Zhang Khuram Shahzad Ruxin Qi Zhenbin Zhang Zhiqi Lu Yue Lu Xiang Yu Muhammad Hammad Zafar Mengzhi Wang Mengzhi Wang Mengzhi Wang Wujun Liu |
| author_facet | Chuang Li Chuang Li Ning Chen Ning Chen Xingxing Zhang Xingxing Zhang Khuram Shahzad Ruxin Qi Zhenbin Zhang Zhiqi Lu Yue Lu Xiang Yu Muhammad Hammad Zafar Mengzhi Wang Mengzhi Wang Mengzhi Wang Wujun Liu |
| author_sort | Chuang Li |
| collection | DOAJ |
| description | Silage is rich in nutrients, which can make up for the lack of seasonal roughage, and has a certain promotion effect on the intensive feeding of ruminants. In addition, silage can maintain the rumen function of ruminants to a certain extent and reduce the risk of rumen acidosis and abomasum translocation. The purpose of this study was to investigate the effects of the mixed silage of Chinese cabbage waste and rice straw (mixed silage) on antioxidant performance, rumen microbial population, and fermentation metabolism of Hu sheep. The 16 healthy Hu sheep (eight rams and eight ewes, 39.11 ± 1.16 kg, 5.5 months) were randomly divided into two groups (the control group and the mixed silage group) with eight animals (four rams and four ewes) in each group. The control group was fed with farm roughage (peanut seedlings, corn husk, and high grain shell) as forage, and the mixed silage group was fed with the mixed silage as forage. The results showed that the mixed silage had no effect on the growth performance of Hu sheep (p > 0.05). Ruminal butyric acid, total volatile fatty acids (TVFA), and ammonia nitrogen (NH3-N) concentration in the mixed silage group were increased, whereas the pH was decreased (p < 0.05). The blood and rumen total antioxidants capacity (T-AOC) concentration in the mixed silage group was higher, and the malondialdehyde (MDA) content in rumen, serum, liver, and kidney was lower than that in the control group (p < 0.05). PCoA and ANOSIM results of Illumina sequencing indicated that the mixed silage affected the bacterial composition of the rumen microbes. The mixed silage increased the proportion of Prevotellaceae UCG-004 which was in a positive correlation with Vitamin C (Vc). In addition, PICRUSt functional prediction analysis showed that ascorbate and aldarate metabolism were up-regulated in the mixed silage group (p < 0.05). In conclusion, higher contents of VC and acid detergent fiber (ADF) in the mixed silage were beneficial to the growth and reproduction of Prevotellaceae UCG-004, resulting in increased production of the butyric acid significantly upregulated the metabolism of ascorbate and aldarate metabolism, thereby improving the antioxidant properties of Hu sheep. |
| first_indexed | 2024-04-13T02:10:39Z |
| format | Article |
| id | doaj.art-9b7e84e5d5a34cb3b87d86e8ef830c67 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T02:10:39Z |
| publishDate | 2022-08-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-9b7e84e5d5a34cb3b87d86e8ef830c672022-12-22T03:07:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-08-011310.3389/fmicb.2022.978940978940Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheepChuang Li0Chuang Li1Ning Chen2Ning Chen3Xingxing Zhang4Xingxing Zhang5Khuram Shahzad6Ruxin Qi7Zhenbin Zhang8Zhiqi Lu9Yue Lu10Xiang Yu11Muhammad Hammad Zafar12Mengzhi Wang13Mengzhi Wang14Mengzhi Wang15Wujun Liu16College of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, ChinaState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, ChinaInstitute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, ChinaState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, ChinaInstitute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, ChinaDepartment of Biosciences, COMSATS University Islamabad, Islamabad, PakistanCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, ChinaInstitute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi, ChinaSilage is rich in nutrients, which can make up for the lack of seasonal roughage, and has a certain promotion effect on the intensive feeding of ruminants. In addition, silage can maintain the rumen function of ruminants to a certain extent and reduce the risk of rumen acidosis and abomasum translocation. The purpose of this study was to investigate the effects of the mixed silage of Chinese cabbage waste and rice straw (mixed silage) on antioxidant performance, rumen microbial population, and fermentation metabolism of Hu sheep. The 16 healthy Hu sheep (eight rams and eight ewes, 39.11 ± 1.16 kg, 5.5 months) were randomly divided into two groups (the control group and the mixed silage group) with eight animals (four rams and four ewes) in each group. The control group was fed with farm roughage (peanut seedlings, corn husk, and high grain shell) as forage, and the mixed silage group was fed with the mixed silage as forage. The results showed that the mixed silage had no effect on the growth performance of Hu sheep (p > 0.05). Ruminal butyric acid, total volatile fatty acids (TVFA), and ammonia nitrogen (NH3-N) concentration in the mixed silage group were increased, whereas the pH was decreased (p < 0.05). The blood and rumen total antioxidants capacity (T-AOC) concentration in the mixed silage group was higher, and the malondialdehyde (MDA) content in rumen, serum, liver, and kidney was lower than that in the control group (p < 0.05). PCoA and ANOSIM results of Illumina sequencing indicated that the mixed silage affected the bacterial composition of the rumen microbes. The mixed silage increased the proportion of Prevotellaceae UCG-004 which was in a positive correlation with Vitamin C (Vc). In addition, PICRUSt functional prediction analysis showed that ascorbate and aldarate metabolism were up-regulated in the mixed silage group (p < 0.05). In conclusion, higher contents of VC and acid detergent fiber (ADF) in the mixed silage were beneficial to the growth and reproduction of Prevotellaceae UCG-004, resulting in increased production of the butyric acid significantly upregulated the metabolism of ascorbate and aldarate metabolism, thereby improving the antioxidant properties of Hu sheep.https://www.frontiersin.org/articles/10.3389/fmicb.2022.978940/fullmixed silageHu sheepantioxidant propertieshigh-throughput sequencingbacterial community |
| spellingShingle | Chuang Li Chuang Li Ning Chen Ning Chen Xingxing Zhang Xingxing Zhang Khuram Shahzad Ruxin Qi Zhenbin Zhang Zhiqi Lu Yue Lu Xiang Yu Muhammad Hammad Zafar Mengzhi Wang Mengzhi Wang Mengzhi Wang Wujun Liu Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep Frontiers in Microbiology mixed silage Hu sheep antioxidant properties high-throughput sequencing bacterial community |
| title | Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep |
| title_full | Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep |
| title_fullStr | Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep |
| title_full_unstemmed | Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep |
| title_short | Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep |
| title_sort | mixed silage with chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen prevotellaceae ucg 004 in hu sheep |
| topic | mixed silage Hu sheep antioxidant properties high-throughput sequencing bacterial community |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.978940/full |
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