The draft genome sequence of “Nitrospira lenta” strain BS10, a nitrite oxidizing bacterium isolated from activated sludge
Abstract The genus Nitrospira is considered to be the most widespread and abundant group of nitrite-oxidizing bacteria in many natural and man-made ecosystems. However, the ecophysiological versatility within this phylogenetic group remains highly understudied, mainly due to the lack of pure culture...
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| Format: | Article |
| Language: | English |
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BMC
2018-11-01
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| Series: | Standards in Genomic Sciences |
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| Online Access: | http://link.springer.com/article/10.1186/s40793-018-0338-7 |
| _version_ | 1818137720988368896 |
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| author | Dimitra Sakoula Boris Nowka Eva Spieck Holger Daims Sebastian Lücker |
| author_facet | Dimitra Sakoula Boris Nowka Eva Spieck Holger Daims Sebastian Lücker |
| author_sort | Dimitra Sakoula |
| collection | DOAJ |
| description | Abstract The genus Nitrospira is considered to be the most widespread and abundant group of nitrite-oxidizing bacteria in many natural and man-made ecosystems. However, the ecophysiological versatility within this phylogenetic group remains highly understudied, mainly due to the lack of pure cultures and genomic data. To further expand our understanding of this biotechnologically important genus, we analyzed the high quality draft genome of “Nitrospira lenta” strain BS10, a sublineage II Nitrospira that was isolated from a municipal wastewater treatment plant in Hamburg, Germany. The genome of “N. lenta” has a size of 3,756,190 bp and contains 3968 genomic objects, of which 3907 are predicted protein-coding sequences. Thorough genome annotation allowed the reconstruction of the “N. lenta” core metabolism for energy conservation and carbon fixation. Comparative analyses indicated that most metabolic features are shared with N. moscoviensis and “N. defluvii”, despite their ecological niche differentiation and phylogenetic distance. In conclusion, the genome of “N. lenta” provides important insights into the genomic diversity of the genus Nitrospira and provides a foundation for future comparative genomic studies that will generate a better understanding of the nitrification process. |
| first_indexed | 2024-12-11T10:00:48Z |
| format | Article |
| id | doaj.art-9b7f1aef5a4b47ed971f90e246d8477e |
| institution | Directory Open Access Journal |
| issn | 1944-3277 |
| language | English |
| last_indexed | 2024-12-11T10:00:48Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Standards in Genomic Sciences |
| spelling | doaj.art-9b7f1aef5a4b47ed971f90e246d8477e2022-12-22T01:12:08ZengBMCStandards in Genomic Sciences1944-32772018-11-011311810.1186/s40793-018-0338-7The draft genome sequence of “Nitrospira lenta” strain BS10, a nitrite oxidizing bacterium isolated from activated sludgeDimitra Sakoula0Boris Nowka1Eva Spieck2Holger Daims3Sebastian Lücker4Department of Microbiology, IWWR, Radboud UniversityDepartment of Microbiology & Biotechnology, University of HamburgDepartment of Microbiology & Biotechnology, University of HamburgDivision of Microbial Ecology, Department of Microbiology and Ecosystem Science, University of ViennaDepartment of Microbiology, IWWR, Radboud UniversityAbstract The genus Nitrospira is considered to be the most widespread and abundant group of nitrite-oxidizing bacteria in many natural and man-made ecosystems. However, the ecophysiological versatility within this phylogenetic group remains highly understudied, mainly due to the lack of pure cultures and genomic data. To further expand our understanding of this biotechnologically important genus, we analyzed the high quality draft genome of “Nitrospira lenta” strain BS10, a sublineage II Nitrospira that was isolated from a municipal wastewater treatment plant in Hamburg, Germany. The genome of “N. lenta” has a size of 3,756,190 bp and contains 3968 genomic objects, of which 3907 are predicted protein-coding sequences. Thorough genome annotation allowed the reconstruction of the “N. lenta” core metabolism for energy conservation and carbon fixation. Comparative analyses indicated that most metabolic features are shared with N. moscoviensis and “N. defluvii”, despite their ecological niche differentiation and phylogenetic distance. In conclusion, the genome of “N. lenta” provides important insights into the genomic diversity of the genus Nitrospira and provides a foundation for future comparative genomic studies that will generate a better understanding of the nitrification process.http://link.springer.com/article/10.1186/s40793-018-0338-7“Nitrospira lenta”Nitrite oxidationNitrospiraWastewater treatment |
| spellingShingle | Dimitra Sakoula Boris Nowka Eva Spieck Holger Daims Sebastian Lücker The draft genome sequence of “Nitrospira lenta” strain BS10, a nitrite oxidizing bacterium isolated from activated sludge Standards in Genomic Sciences “Nitrospira lenta” Nitrite oxidation Nitrospira Wastewater treatment |
| title | The draft genome sequence of “Nitrospira lenta” strain BS10, a nitrite oxidizing bacterium isolated from activated sludge |
| title_full | The draft genome sequence of “Nitrospira lenta” strain BS10, a nitrite oxidizing bacterium isolated from activated sludge |
| title_fullStr | The draft genome sequence of “Nitrospira lenta” strain BS10, a nitrite oxidizing bacterium isolated from activated sludge |
| title_full_unstemmed | The draft genome sequence of “Nitrospira lenta” strain BS10, a nitrite oxidizing bacterium isolated from activated sludge |
| title_short | The draft genome sequence of “Nitrospira lenta” strain BS10, a nitrite oxidizing bacterium isolated from activated sludge |
| title_sort | draft genome sequence of nitrospira lenta strain bs10 a nitrite oxidizing bacterium isolated from activated sludge |
| topic | “Nitrospira lenta” Nitrite oxidation Nitrospira Wastewater treatment |
| url | http://link.springer.com/article/10.1186/s40793-018-0338-7 |
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