Isolation and Characterization of 20 Polymorphic Microsatellite Markers for <i>Juglans mandshurica</i> (Juglandaceae)

Premise of the study: Fifty microsatellite loci were developed for the endangered species <i>Juglans mandshurica</i> to investigate its genetic diversity and population structure. Methods and Results: In all, 50 microsatellite markers were isolated from <i>J. mandshurica</i>...

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Bibliographic Details
Main Authors: Chang-Mei Chen, Shi-Jie Han, Shu-Sheng Yuan, Chun-Jing Wang, Jing-Hua Yu
Format: Article
Language:English
Published: Wiley 2013-06-01
Series:Applications in Plant Sciences
Subjects:
Online Access:http://www.bioone.org/doi/full/10.3732/apps.1200009
Description
Summary:Premise of the study: Fifty microsatellite loci were developed for the endangered species <i>Juglans mandshurica</i> to investigate its genetic diversity and population structure. Methods and Results: In all, 50 microsatellite markers were isolated from <i>J. mandshurica</i>, using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol. Twenty of these polymorphic markers were assessed in samples collected from 98 individuals among five populations in northeastern China. Across all of the <i>J. mandshurica</i> samples, the number of alleles per locus ranged from one to 17. Conclusions: These new microsatellite loci will be useful for conservation genetics studies of <i>J. mandshurica</i>.
ISSN:2168-0450