Wound healing by topical application of Momordica charantia L. formulations on mice
Background and Aim: Momordica charantia is mainly characterized by its antimicrobial and antioxidant properties. The current study aimed to evaluate the healing activity of gel and cream formulations based on M. charantia on induced wounds in mice. Materials and Methods: Acetonic extract of M. ch...
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| Format: | Article |
| Language: | English |
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Veterinary World
2021-10-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.14/October-2021/17.pdf |
| _version_ | 1818897687224778752 |
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| author | William Antonio Sagástegui-Guarniz Carmen R. Silva-Correa Víctor E. Villarreal-La Torre María V. González-Blas Walter O. Sagástegui-Guarniz Abhel A. Calderón-Peña Cinthya L. Aspajo-Villalaz José L. Cruzado-Razco Julio Hilario-Vargas |
| author_facet | William Antonio Sagástegui-Guarniz Carmen R. Silva-Correa Víctor E. Villarreal-La Torre María V. González-Blas Walter O. Sagástegui-Guarniz Abhel A. Calderón-Peña Cinthya L. Aspajo-Villalaz José L. Cruzado-Razco Julio Hilario-Vargas |
| author_sort | William Antonio Sagástegui-Guarniz |
| collection | DOAJ |
| description | Background and Aim: Momordica charantia is mainly characterized by its antimicrobial and antioxidant properties. The current study aimed to evaluate the healing activity of gel and cream formulations based on M. charantia on induced wounds in mice.
Materials and Methods: Acetonic extract of M. charantia was prepared and incorporated into gel and cream formulations. Mus musculus Balb/c (n=30) with induced injury were distributed into five groups: Group I (control – day 7), Group II (control – day 14), Group III (1% gel – day 7), and Group IV (1% gel – day 14) to which 1% M. charantia gel was dermally applied daily for 7 and 14 days, respectively, Group V (1% cream – day 7) and Group VI (1% cream – day 14) to which of M. charantia 1% cream were dermally applied daily for 7 and 14 days, respectively. Time of wound closure was determined during the experimentation; rats were euthanized with sodium pentobarbital 60 mg/kg/pc v.ip. for obtaining skin samples for histopathological analysis.
Results: Groups IV and VI showed a higher percentage of wound closure on day 14, and in histopathological analysis, effect was greater in Group VI with the presence of fibroblasts and abundant collagen and elastic fibers.
Conclusion: M. charantia gel and cream showed wound healing activity on induced wounded mice; the most effective treatment was M. charantia 1% cream formulation. |
| first_indexed | 2024-12-19T19:20:08Z |
| format | Article |
| id | doaj.art-9b83b211bc624b4aa238b1801c712ad4 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-19T19:20:08Z |
| publishDate | 2021-10-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-9b83b211bc624b4aa238b1801c712ad42022-12-21T20:09:02ZengVeterinary WorldVeterinary World0972-89882231-09162021-10-0114102699270410.14202/vetworld.2021.2699-2704Wound healing by topical application of Momordica charantia L. formulations on miceWilliam Antonio Sagástegui-Guarniz0https://orcid.org/0000-0002-4582-9547Carmen R. Silva-Correa1https://orcid.org/0000-0001-9866-2739Víctor E. Villarreal-La Torre2https://orcid.org/0000-0003-3956-3559María V. González-Blas3https://orcid.org/0000-0002-6168-3568Walter O. Sagástegui-Guarniz4https://orcid.org/0000-0003-4562-4004Abhel A. Calderón-Peña5https://orcid.org/0000-0001-7953-9874Cinthya L. Aspajo-Villalaz6https://orcid.org/0000-0002-7776-5488José L. Cruzado-Razco7https://orcid.org/0000-0001-8731-283XJulio Hilario-Vargas8https://orcid.org/0000-0002-8656-6843Department of Pharmacology, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Peru.Department of Pharmacology, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Peru.Department of Pharmacology, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Peru.Department of Biochemistry, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Peru.Graduate School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Peru.Department of Biological Chemistry and Animal Physiology, School of Biological Science, National University of Trujillo, Trujillo, Peru.Department of Biological Chemistry and Animal Physiology, School of Biological Science, National University of Trujillo, Trujillo, Peru.Department of Pharmacology, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Peru.Department of Physiology, School of Medicine, National University of Trujillo, Trujillo, Peru.Background and Aim: Momordica charantia is mainly characterized by its antimicrobial and antioxidant properties. The current study aimed to evaluate the healing activity of gel and cream formulations based on M. charantia on induced wounds in mice. Materials and Methods: Acetonic extract of M. charantia was prepared and incorporated into gel and cream formulations. Mus musculus Balb/c (n=30) with induced injury were distributed into five groups: Group I (control – day 7), Group II (control – day 14), Group III (1% gel – day 7), and Group IV (1% gel – day 14) to which 1% M. charantia gel was dermally applied daily for 7 and 14 days, respectively, Group V (1% cream – day 7) and Group VI (1% cream – day 14) to which of M. charantia 1% cream were dermally applied daily for 7 and 14 days, respectively. Time of wound closure was determined during the experimentation; rats were euthanized with sodium pentobarbital 60 mg/kg/pc v.ip. for obtaining skin samples for histopathological analysis. Results: Groups IV and VI showed a higher percentage of wound closure on day 14, and in histopathological analysis, effect was greater in Group VI with the presence of fibroblasts and abundant collagen and elastic fibers. Conclusion: M. charantia gel and cream showed wound healing activity on induced wounded mice; the most effective treatment was M. charantia 1% cream formulation.http://www.veterinaryworld.org/Vol.14/October-2021/17.pdfhistologymomordica charantiaskintopical administrationwound healing |
| spellingShingle | William Antonio Sagástegui-Guarniz Carmen R. Silva-Correa Víctor E. Villarreal-La Torre María V. González-Blas Walter O. Sagástegui-Guarniz Abhel A. Calderón-Peña Cinthya L. Aspajo-Villalaz José L. Cruzado-Razco Julio Hilario-Vargas Wound healing by topical application of Momordica charantia L. formulations on mice Veterinary World histology momordica charantia skin topical administration wound healing |
| title | Wound healing by topical application of Momordica charantia L. formulations on mice |
| title_full | Wound healing by topical application of Momordica charantia L. formulations on mice |
| title_fullStr | Wound healing by topical application of Momordica charantia L. formulations on mice |
| title_full_unstemmed | Wound healing by topical application of Momordica charantia L. formulations on mice |
| title_short | Wound healing by topical application of Momordica charantia L. formulations on mice |
| title_sort | wound healing by topical application of momordica charantia l formulations on mice |
| topic | histology momordica charantia skin topical administration wound healing |
| url | http://www.veterinaryworld.org/Vol.14/October-2021/17.pdf |
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