Association between lncRNAs in plasma exosomes and diabetic retinopathy
BackgroundLong noncoding RNA (lncRNA) in plasma exosomes is a potential non-invasive diagnostic biomarker for diabetic retinopathy (DR). However, the changes in plasma exosomal lncRNAs and diagnostic relevance in patients with DR patients remain unclear.MethodsA case–control study with type 2 diabet...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.987488/full |
| _version_ | 1828231005551460352 |
|---|---|
| author | Qingqing Ye Lian Li Zhoujie Shao Miao Xu Li Li Qianqian Yan Bin Huang Tian Zhao |
| author_facet | Qingqing Ye Lian Li Zhoujie Shao Miao Xu Li Li Qianqian Yan Bin Huang Tian Zhao |
| author_sort | Qingqing Ye |
| collection | DOAJ |
| description | BackgroundLong noncoding RNA (lncRNA) in plasma exosomes is a potential non-invasive diagnostic biomarker for diabetic retinopathy (DR). However, the changes in plasma exosomal lncRNAs and diagnostic relevance in patients with DR patients remain unclear.MethodsA case–control study with type 2 diabetes mellitus (T2DM) and patients with comorbid DR were enrolled, and their clinical information and blood samples were collected. Plasma exosomes were extracted, and the relative expression levels of representative differentially expressed exosomal lncRNAs were determined. A logistic regression model was used to analyze the relationships of DR with relative lncRNA expression and DR-related factors, and receiver operating characteristic (ROC) curve analysis was used to evaluate the value of exosomal lncRNAs for DR diagnosis.ResultsSixty-two patients with T2DM and sixty-two patients with DR were matched by age, sex, and disease duration. The fasting blood glucose concentration, glycosylated hemoglobin level (HbA1c), and relative expression of the plasma exosomal lncRNA DLX6-AS1 were significantly higher in the DR group than in the T2DM group, whereas the 2-h C-peptide concentration and relative expression of the lncRNAs PRINS and FAM190A-3 were lower in the DR group. After adjusting for relevant confounders, the fasting blood glucose concentration, HbA1c level, 2-h C-peptide concentration, and relative expression of lncRNA DLX6-AS1, PRINS, and FAM190A-3 were found to be associated with DR. Both DLX6-AS1 [area under the curve (AUC): 0.658 (0.562–0.754)], PRINS [AUC: 0.798 (0.722–0.873)], and FAM190A-3 [AUC: 0.603 (0.503-0.702)] expression had predictive value for DR diagnosis. The combination of DLX6-AS1 and PRINS yielded an AUC of 0.813 (0.740–0.886). In males, the combination of DLX6-AS1 and PRINS yielded an AUC of 0.860 (0.780–0.940).ConclusionThe fasting blood glucose concentration, HbA1c level, and exosomal DLX6-AS1 expression were identified as risk factors for DR, whereas the 2-h C-peptide concentration and exosomal PRINS and FAM190A-3 were identified as protective against DR. The combination of exosomal DLX6-AS1 and PRINS had good diagnostic value for DR in the general population and males. More attention should be paid to the role of exosomal PRINS expression as a predictive and diagnostic DR biomarker in females. |
| first_indexed | 2024-04-12T18:56:56Z |
| format | Article |
| id | doaj.art-9b84ada2f9f443d29af6cad1308bef73 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-12T18:56:56Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-9b84ada2f9f443d29af6cad1308bef732022-12-22T03:20:17ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-09-011310.3389/fendo.2022.987488987488Association between lncRNAs in plasma exosomes and diabetic retinopathyQingqing Ye0Lian Li1Zhoujie Shao2Miao Xu3Li Li4Qianqian Yan5Bin Huang6Tian Zhao7Clinical Laboratory, Beilun District People’s Hospital, Ningbo, ChinaDepartment of Prevention and Control, Ningbo Kangning Hospital, Ningbo, ChinaClinical Laboratory, Beilun District People’s Hospital, Ningbo, ChinaDepartment of Endocrinology, Ningbo First hospital, Ningbo, ChinaDepartment of Endocrinology, Ningbo First hospital, Ningbo, ChinaDepartment of Global Health, Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences, Ningbo, ChinaDepartment of Emergency Medicine, Beilun District People’s Hospital, Ningbo, ChinaDepartment of Global Health, Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences, Ningbo, ChinaBackgroundLong noncoding RNA (lncRNA) in plasma exosomes is a potential non-invasive diagnostic biomarker for diabetic retinopathy (DR). However, the changes in plasma exosomal lncRNAs and diagnostic relevance in patients with DR patients remain unclear.MethodsA case–control study with type 2 diabetes mellitus (T2DM) and patients with comorbid DR were enrolled, and their clinical information and blood samples were collected. Plasma exosomes were extracted, and the relative expression levels of representative differentially expressed exosomal lncRNAs were determined. A logistic regression model was used to analyze the relationships of DR with relative lncRNA expression and DR-related factors, and receiver operating characteristic (ROC) curve analysis was used to evaluate the value of exosomal lncRNAs for DR diagnosis.ResultsSixty-two patients with T2DM and sixty-two patients with DR were matched by age, sex, and disease duration. The fasting blood glucose concentration, glycosylated hemoglobin level (HbA1c), and relative expression of the plasma exosomal lncRNA DLX6-AS1 were significantly higher in the DR group than in the T2DM group, whereas the 2-h C-peptide concentration and relative expression of the lncRNAs PRINS and FAM190A-3 were lower in the DR group. After adjusting for relevant confounders, the fasting blood glucose concentration, HbA1c level, 2-h C-peptide concentration, and relative expression of lncRNA DLX6-AS1, PRINS, and FAM190A-3 were found to be associated with DR. Both DLX6-AS1 [area under the curve (AUC): 0.658 (0.562–0.754)], PRINS [AUC: 0.798 (0.722–0.873)], and FAM190A-3 [AUC: 0.603 (0.503-0.702)] expression had predictive value for DR diagnosis. The combination of DLX6-AS1 and PRINS yielded an AUC of 0.813 (0.740–0.886). In males, the combination of DLX6-AS1 and PRINS yielded an AUC of 0.860 (0.780–0.940).ConclusionThe fasting blood glucose concentration, HbA1c level, and exosomal DLX6-AS1 expression were identified as risk factors for DR, whereas the 2-h C-peptide concentration and exosomal PRINS and FAM190A-3 were identified as protective against DR. The combination of exosomal DLX6-AS1 and PRINS had good diagnostic value for DR in the general population and males. More attention should be paid to the role of exosomal PRINS expression as a predictive and diagnostic DR biomarker in females.https://www.frontiersin.org/articles/10.3389/fendo.2022.987488/fulltype 2 diabetes mellitusdiabetic retinopathyexosomelncRNAcase–control study |
| spellingShingle | Qingqing Ye Lian Li Zhoujie Shao Miao Xu Li Li Qianqian Yan Bin Huang Tian Zhao Association between lncRNAs in plasma exosomes and diabetic retinopathy Frontiers in Endocrinology type 2 diabetes mellitus diabetic retinopathy exosome lncRNA case–control study |
| title | Association between lncRNAs in plasma exosomes and diabetic retinopathy |
| title_full | Association between lncRNAs in plasma exosomes and diabetic retinopathy |
| title_fullStr | Association between lncRNAs in plasma exosomes and diabetic retinopathy |
| title_full_unstemmed | Association between lncRNAs in plasma exosomes and diabetic retinopathy |
| title_short | Association between lncRNAs in plasma exosomes and diabetic retinopathy |
| title_sort | association between lncrnas in plasma exosomes and diabetic retinopathy |
| topic | type 2 diabetes mellitus diabetic retinopathy exosome lncRNA case–control study |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.987488/full |
| work_keys_str_mv | AT qingqingye associationbetweenlncrnasinplasmaexosomesanddiabeticretinopathy AT lianli associationbetweenlncrnasinplasmaexosomesanddiabeticretinopathy AT zhoujieshao associationbetweenlncrnasinplasmaexosomesanddiabeticretinopathy AT miaoxu associationbetweenlncrnasinplasmaexosomesanddiabeticretinopathy AT lili associationbetweenlncrnasinplasmaexosomesanddiabeticretinopathy AT qianqianyan associationbetweenlncrnasinplasmaexosomesanddiabeticretinopathy AT binhuang associationbetweenlncrnasinplasmaexosomesanddiabeticretinopathy AT tianzhao associationbetweenlncrnasinplasmaexosomesanddiabeticretinopathy |